Atp assay kit
The ATP assay kit is a laboratory tool used to measure the levels of adenosine triphosphate (ATP) in a sample. ATP is a critical molecule involved in cellular energy production and is commonly used as an indicator of the overall metabolic activity of cells or tissues.
Lab products found in correlation
35 protocols using atp assay kit
Quantification of Tissue ATP Levels
Extracellular ATP and HMGB1 Release after IR
Intracellular Metabolite Profiling of Cancer Cells
Comprehensive Metabolic Analysis of Ovarian Cancer
Glucose Uptake Assay Kit (Biovision, K676-100), Lactate Assay Kit (Biovision, K667-100), ATP Assay Kit (Promega, FF2000), and NADPH Assay Kit (AAT Bioquest; AAT-15272) were used to examine the products of glycolysis in the OC cells referring to the instructions.
An untargeted metabolomic analysis was performed using mass spectrometry, which involves the following steps: sample pretreatment, metabolite extraction, full scan detection in liquid chromatography-tandem mass spectrometry, data pretreatment, statistical analysis, and differential structure identification. Approximately 1 × 107 cells from each sample were used with six replicates. The cells were fixed with 1-mL mixture (2methanol: 2acetonitrile: 1water). Metabolomic analysis and mass spectrometry were performed by Shanghai Applied Protein Technology.
Metabolic Profiling of Cell Cultures
Quantifying Cellular ATP Levels
Lung Tissue Oxidative Stress Analysis
Bacterial ATP Concentration Quantification
aerobic conditions with shaking [2 (link), 16 (link)]. After checking growth levels
spectrophotometrically at 590 nm, the culture media was centrifuged at 13,000 ×g for 5 min
and the supernatants collected. To remove the bacterial cells, supernatants were filtered
with a 0.22-µm membrane (Millipore Japan, Tokyo, Japan). ATP
concentrations in culture supernatants were measured using an ATP assay kit (Promega, WI,
USA) according to the manufacturer’s protocols.
ATP Quantification in Mitochondrial Extracts
Cell Viability Assay of VLX600
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