Cbm30a system

Manufactured by Thermo Fisher Scientific

Sourced in Japan

The CBM30A system is a highly sensitive and accurate instrument designed for liquid chromatography-mass spectrometry (LC-MS) applications. It functions as a modular communication interface, enabling seamless integration between various Thermo Scientific instruments and data systems. The CBM30A system facilitates the efficient transfer of data and commands, ensuring reliable and reproducible results across your laboratory workflows.

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Lab products found in correlation

6500 q trap, by Thermo Fisher Scientific (14 mentions) Acquity uplc hss t3 c18, by Waters Corporation (12 mentions) 4500 q trap, by Thermo Fisher Scientific (8 mentions) Acquity uplc hss t3 c18 column, by Waters Corporation (5 mentions) Analyst 1, by AB Sciex (3 mentions) Scaa 104, by ANPEL (2 mentions) Cnwbond carbon gcb spe cartridge, by ANPEL (2 mentions) 6500 triple quadrupole, by Thermo Fisher Scientific (1 mentions) Acquity uplc hss t3, by Waters Corporation (1 mentions) 6500 q trap, by Waters Corporation (1 mentions) 6500 q trap, by Shimadzu (1 mentions) 6500 quadrupole trap, by Thermo Fisher Scientific (1 mentions) 4500 q trap, by AB Sciex (1 mentions) Shim pack uflc, by Shimadzu (1 mentions) Api 6500 q trap lc ms ms system, by AB Sciex (1 mentions) 4500 qtrap, by Waters Corporation (1 mentions)

30 protocols using cbm30a system

Phytochemical Analysis of Petal Extracts

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The petal extracts were analyzed using an LC–ESI–MS/MS system (high-performance liquid chromatography (HPLC) Shim-pack UFLC SHIMADZU CBM30A system; MS, Applied Biosystems 6500 Triple Quadrupole). The analysis was performed using YMC C30 columns (3 µm, 2 mm × 100 mm) and an acetonitrile:methanol (3:1, v/v) (0.01% BHT):methyl tert-butyl ether (0.01% BHT) solvent. The solvent gradient was as follows: 85:5 (v/v) at 0 min, 75:25 V/V at 2 min, 40:60 (v/v) at 2 min 30 s, 5:95 (v/v) at 3 min, 5:95 (v/v) at 4 min, 85:15 (v/v) at 4 min, and 85:15 (v/v) at 6 min. The flow rate was 0.8 mL/min. The temperature was maintained at 28 °C, and the injection volume was 5 μL.
The effluent was alternatively connected to an ESI-triple quadrupole-linear ion trap (Q TRAP)-MS (API 6500 Q TRAP LC/MS/MS System) equipped with an APCI Turbo Ion-Spray interface operating in a negative ion mode. The equipment was controlled using Analyst 1.6.3 software (AB Sciex). The APCI source operation parameters were as follows: ion source, turbo spray; source temperature 350 °C; curtain gas (CUR) was set at 25.0 psi; and collision gas (CAD) was medium. The DP and CE settings were further optimized for individual MRM transitions. A specific set of MRM transitions were monitored for each period, according to the plant hormones eluted.

Zhang H., Zhang S., Zhang H., Chen X., Liang F., Qin H., Zhang Y., Cong R., Xin H, & Zhang Z. (2020). Carotenoid metabolite and transcriptome dynamics underlying flower color in marigold (Tagetes erecta L.). Scientific Reports, 10, 16835.

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IAA Quantification by HPLC-MS/MS

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Plant materials used for IAA measurement were the same as materials for RNA-seq. To determine the IAA content in each sample, the HPLC-MS/MS system (HPLC, Shim-pack UFLC SHIMADZU CBM30A system⁶; MS, Applied Biosystems 4500 Triple Quadrupole⁷) analyses were carried out.

Guo J., Cao K., Li Y., Yao J.L., Deng C., Wang Q., Zhu G., Fang W., Chen C., Wang X., Guan L., Ding T, & Wang L. (2018). Comparative Transcriptome and Microscopy Analyses Provide Insights into Flat Shape Formation in Peach (Prunus persica). Frontiers in Plant Science, 8, 2215.

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Anthocyanin Analysis via LC-ESI-MS/MS

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The anthocyanins extracted from the freeze-dried storage roots were analyzed using a Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry (LC-ESI-MS/MS) system (HPLC, UFLC SHIMADZU CBM30A system, MS, Applied Biosystems 4500 QTrap) (Wang et al., 2018 (link)). Twenty one standards developed by ChromaDex company name cyanidin 3-(6″-caffeoylsophoroside)-5-glucoside, cyanidin 3-p-hydroxybenzoylsophoroside-5-glucoside were used for quantification of anthocyanins (Supplementary Table 3).

Yan H., Zhang Y., Ahmad M.Q., Liu Y., Kou M., Ma M., Li C., Arisha M.H., Tang W., Wang X., Gao R., Song W., Li Z, & Li Q. (2022). Comparative Analysis of Anthocyanin Compositions and Starch Physiochemical Properties of Purple-Fleshed Sweetpotato “Xuzishu8” in Desert Regions of China. Frontiers in Plant Science, 13, 841969.

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LC-ESI-MS/MS Analysis of Samples

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The sample extracts were analyzed using an LC-ESI-MS/MS system (UPLC, Shim-pack UFLC SHIMADZU CBM30A system; MS, Applied Biosystems 6500 QTRAP). The UPLC conditions were performed according to the method by Wang et al. [37 (link)]. The analytical conditions were as follows, UPLC: column, Waters ACQUITY UPLC HSS T3 C18 (1.8 µm, 2.1 mm × 100 mm, Milford, MA, USA); solvent system, water (0.04% acetic acid): acetonitrile (0.04% acetic acid); gradient program, 100:0 v/v at 0 min, 5:95 v/v at 11.0 min, 5:95 v/v at 12.0 min, 95:5 v/v at 12.1 min, 95:5 v/v at 15.0 min; flow rate, 0.40 mL/min; temperature, 40 °C; injection volume: 2 μL. The effluent was alternatively connected to an ESI-triple quadrupole-linear ion trap (Q TRAP)-MS.

Wang F., Chen L., Chen H., Chen S, & Liu Y. (2019). Analysis of Flavonoid Metabolites in Citrus Peels (Citrus reticulata “Dahongpao”) Using UPLC-ESI-MS/MS. Molecules, 24(15), 2680.

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LC-ESI-MS/MS Based Metabolite Analysis

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The sample extracts were analyzed using LC-ESI-MS/MS (UPLC, Shim-pack UFLC SHIMADZU CBM30A system; MS, Applied Biosystems 6500 QTRAP, America), based on the method published by Chen et al [23 ]. The sample components were separated on a Waters ACQUITY UPLC HSS T3 C18 column (1.8 μm, 2.1 mm × 100 mm; Waters, America) using a mixture of water (0.04% acetic acid) and acetonitrile (0.04% acetic acid) as mobile phase. The gradient program was set to 100:0 v/v water:acetonitrile at 0 min, 5:95 v/v at 11.0 min, 5:95 v/v at 12.0 min, 95:5 v/v at 12.1 min, and 95:5v/v at 15.0 min. The chromatographic analyses were performed at 40°C and 0.40 mL/min flow rate, and the injection volume was set to 2 μL. The effluent was alternately connected to an ESI-triple quadrupole-linear ion trap (Q TRAP)-MS [24 (link)].

Wang F., Chen L., Chen S., Chen H, & Liu Y. (2021). Characterization of two closely related citrus cultivars using UPLC-ESI-MS/MS-based widely targeted metabolomics. PLoS ONE, 16(7), e0254759.

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LC-ESI-MS Analysis of Extracts

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An LC-ESI-MS system (high-performance liquid chromatography (HPLC) and Shim-pack ultraperformance liquid chromatography (UFLC), Shimadzu CBM30A system; mass spectrometry (MS), Applied Biosystems 6500 triple quadrupole-linear ion trap (QTRAP)) was used to analyze the resulting extracts. The following analytical conditions were employed: HPLC column: Waters ACQUITY UPLC HSS T3 C18 (1.8 μm, 2.1 mm∗100 mm); solvent system: water (0.04% acetic acid) and acetonitrile (0.04% acetic acid); and gradient program: 100 : 0 V/V at 0 min, 5 : 95 V/V at 11.0 min, 5 : 95 V/V at 12.0 min, 95 : 5 V/V at 12.1 min, and 95 : 5 V/V at 15.0 min. A flow rate of 0.40 mL/min was utilized; the temperature was maintained at 40°C, and 2 μL injection volume was used. The effluent was alternately connected to an electrospray ionization-triple quadrupole-linear ion trap-mass spectrometry (ESI-QTRAP-MS/MS).

Meng M., Bai C., Wan B., Zhao L., Li Z., Li D, & Zhang S. (2021). A Network Pharmacology-Based Study on Irritable Bowel Syndrome Prevention and Treatment Utilizing Shenling Baizhu Powder. BioMed Research International, 2021, 4579850.

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LC-ESI-MS/MS Analysis of Samples

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The sample extracts were analyzed using a LC-ESI-MS/MS system (HPLC, Shim-pack UFLC SHIMADZU CBM30A system, Kyoto, Japan; MS, Applied Biosystems 4500 QTRAP, Foster city, CA, USA). The analytical conditions were as follows: HPLC: column, Waters ACQUITY UPLC HSS T3 C18 (1.8 µm, 2.1 mm × 100 mm; Milford, MA, USA); solvent system, solvent A (water, 0.04% acetic acid): solvent B (acetonitrile, 0.04% acetic acid); gradient program, 100:0 (v/v) at 0 min, 5:95 (v/v) at 11.0 min, 5:95 (v/v) at 12.0 min, 95:5 (v/v) at 12.1 min, 95:5 (v/v) at 15.0 min; flow rate, 0.40 mL·min⁻¹; temperature, 40 °C; injection volume: 5 μL. The effluent was alternatively connected to an ESI-triple quadrupole-linear ion trap (Q Trap)-MS.
Quality control (QC) samples were prepared from the mixture of all samples, then divided into four samples and analyzed under the same conditions as the experimental samples. The QC samples were injected every six experimental samples throughout the analytical procedure, to provide a set of data from which repeatability could be assessed.

Yang F., Yang T., Liu K., Yang Q., Wan Y., Wang R, & Li G. (2019). Analysis of Metabolite Accumulation Related to Pod Color Variation of Caragana intermedia. Molecules, 24(4), 717.

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Flavonoid Metabolomics Analysis Pipeline

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The flavonoid metabolomics of samples were analyzed as described previously [3 (link)], using a UPLC-ESI-MS/MS system (UPLC, Shim-pack UFLC SHIMADZU CBM30A system; MS, Applied Biosystems 6500 Q TRAP, Foster City, USA) equipped with a C18 chromatographic column (Waters ACQUITY UPLC HSS T3, 2.1 mm × 100 mm, 1.8 µm). Phase A of the mobile phase (0.04% acetic acid in water) and phase B of the mobile phase (0.04% acetic acid in acetonitrile) were used as solvent systems. The following was the gradient program (A:B): 95:5 (v/v) at 0 min, 5:95 (v/v) at 11.0 min, 5:95 (v/v) at 12.0 min, 95:5 (v/v) at 12.1 min, 95:5 (v/v) at 15.0 min, and 95:5 (v/v) at 15.0 min; flow rate 0.40 mL/min. The injection volume was 2.0 µL, and the column temperature was held at 40 °C. A Q TRAP-MS was used to analyze the effluent.

Tang J., Li X., Zhang Y., Yang Y., Sun R., Li Y., Gao J, & Han Y. (2022). Differential Flavonoids and Carotenoids Profiles in Grains of Six Poaceae Crops. Foods, 11(14), 2068.

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Metabolic Profiling by LC-MS/MS

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Widely targeted metabolic profiling analyses were performed with an LC-ESI-MS/MS system (HPLC, Shim-pack UFLC SHIMADZU CBM30A system; MS, Applied Biosystems 6500 Q TRAP; Shimadzu, Kyoto, Japan). The analytical conditions were as follows: HPLC: column, ACQUITY UPLC HSS T3 C18 (1.8 µm, 2.1 mm × 100 mm, Waters, Shanghai, China); solvent system, water (0.04% acetic acid): acetonitrile (0.04% acetic acid); gradient program, 95:5 v/v at 0 min, 5:95 v/v at 11.0 min, 5:95 v/v at 12.0 min, 95:5 v/v at 12.1 min, 95:5 v/v at 15.0 min; flow rate, 0.40 mL/min; temperature, 40 °C; injection volume, 2 μL. The effluent was alternatively connected to an ESI-triple quadrupole-linear ion trap (Q TRAP)-MS.

Zhao Y., Zhai G., Li X., Tao H., Li L., He Y., Zhang X., Wang F., Hong G, & Zhu Y. (2022). Metabolomics Reveals Nutritional Diversity among Six Coarse Cereals and Antioxidant Activity Analysis of Grain Sorghum and Sweet Sorghum. Antioxidants, 11(10), 1984.

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LC-ESI-MS/MS Metabolite Profiling

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The sample extracts were analyzed using an LC-ESI-MS/MS system (HPLC, Shim-pack UFLC SHIMADZU CBM30A system, Kyoto, Japan; MS, Applied Biosystems 6500 Q TRAP, San Diego, CA, USA). The HPLC effluent was alternatively connected to an electrospray ionization (ESI)-triple quadrupole-linear ion trap–MS/MS system (Applied Biosystems 4500 Q TRAP, San Diego, CA, USA). The analytical conditions were following descriptions of Chen et al. [40 (link)].
Metabolite identification was based on the Metware MWDB database, following their standard metabolic operating procedures. Metabolite quantification was carried out using multiple-reaction monitoring (MRM) [40 (link)].

Wang Y., Liang X., Li Y., Fan Y., Li Y., Cao Y., An W., Shi Z., Zhao J, & Guo S. (2020). Changes in Metabolome and Nutritional Quality of Lycium barbarum Fruits from Three Typical Growing Areas of China as Revealed by Widely Targeted Metabolomics. Metabolites, 10(2), 46.

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