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Anti granzyme a pe

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Anti-Granzyme A-PE is a fluorescent-labeled antibody that specifically binds to the Granzyme A protein. Granzyme A is a serine protease that plays a role in cell-mediated cytotoxicity. The PE conjugate allows for the detection and analysis of Granzyme A-expressing cells using flow cytometry.

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Lab products found in correlation

Anti cd3 percp cy5, by BioLegend (2 mentions) Anti cd8 apc cy7, by BioLegend (2 mentions) Anti cd4 pe cy7, by BioLegend (2 mentions) Anti perforin apc, by BioLegend (2 mentions) Cytofix cytoperm, by BD (2 mentions) Golgiplug, by BD (2 mentions) Perm buffer, by BD (2 mentions) Anti cxcr3 apc, by BioLegend (1 mentions) Anti ccr5 pe, by BioLegend (1 mentions) Anti cxcr4 pe, by BioLegend (1 mentions) Anti ccr7 apc, by BioLegend (1 mentions) Anti tnf α apc, by BioLegend (1 mentions) Anti ifn γ pe, by BioLegend (1 mentions) Anti ifn γ pe cy7, by BioLegend (1 mentions) Pe anti vδ2, by BioLegend (1 mentions) Il 17a pe cy7, by BioLegend (1 mentions) Anti tnf α pe, by BioLegend (1 mentions) Anti perforin fitc, by BioLegend (1 mentions) Anti cd3 v500, by BD (1 mentions) Anti cd56 apc, by BD (1 mentions)

3 protocols using anti granzyme a pe

1

Flow Cytometry for Immune Cell Profiling

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The following Abs were used for short-term culture or surface marker and ICS for flow cytometry (all Abs were from Biolegend): anti-CD3-PerCP-cy5.5, anti-CD8-APC-Cy7, anti-CD4-PE-Cy7, anti-CCR5-PE, anti-CXCR3-APC, anti-CXCR4-PE, anti-CCR7-APC, anti-granzyme A-PE, anti-Perforin-APC, anti-IFN-γ-PE, and anti-TNF-α-APC. The reagents listed below were all commercial products: brefeldin A (GolgiPlug, BD Biosciences), Cytofix/Cytoperm (BD Biosciences), and Perm buffer (BD Biosciences).
Zou S., Tan Y., Xiang Y., Liu Y., Zhu Q., Wu S., Guo W., Luo M., Shen L, & Liang K. (2022). The Role of CD4+CD8+ T Cells in HIV Infection With Tuberculosis. Frontiers in Public Health, 10, 895179.
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2

Multiparameter Flow Cytometry Analysis

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The following antibodies (Abs) were used for short-term culture or surface marker and intracellular cytokine staining for flow cytometry (all Abs were from Biolegend): anti-CD3-PerCP-cy5.5 (Clone UCHT1), anti-CD8-APC-Cy7 (Clone SK1), anti-CD4-PE-Cy7 (Clone RPA-T4), anti-Vδ1-APC (Clone REA173), anti-Vδ2-PE (Clone B6), anti-Vγ2-FITC (Clone 7A5), anti-TNF-α-PE (Clone MAb11), anti-IFN-γ-PE-Cy7 (Clone 4S.B3), anti-interleukin-17A (IL-17A)-PE-Cy7 (Clone BL168), anti-Granzyme A-PE (Clone CB9), anti-Perforin-APC (Clone dG9), anti-granulocyte macrophage colony-stimulating factor (GM-CSF)-APC (Clone BVD2-21C11). The isotype control mAbs were purchased from the related company, respectively. The reagents listed below were all commercial products: brefeldin A (GolgiPlug, BD Biosciences), Cytofix/Cytoperm, and Perm buffer (BD Biosciences). The isotype control mAbs were purchased from the related company, respectively.
Zou S., Xiang Y., Guo W., Zhu Q., Wu S., Tan Y., Yan Y., Shen L., Feng Y, & Liang K. (2022). Phenotype and function of peripheral blood γδ T cells in HIV infection with tuberculosis. Frontiers in Cellular and Infection Microbiology, 12, 1071880.
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3

Cytotoxic Immune Cell Profiling

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Frozen PBMC samples from all 10 trial subjects isolated prior to first administration of IAD and on day 14 of trial were thawed and stained with anti-CD3-V500 (BD, Oxford U.K) and anti-CD56-APC (BD, Oxford U.K) for 30 mins on ice before fixing/permeabilising (as above) and then staining with anti-Granzyme A-PE (Biolegend, London, U.K). and anti-Perforin-FITC (Biolegend, London, U.K) for a further 30 mins on ice prior to analysis by flow cytometry.
Rumel Ahmed S., Newman A.S., O'Daly J., Duffy S., Grafton G., Brady C.A., John Curnow S., Barnes N.M, & Gordon J. (2017). Inosine Acedoben Dimepranol promotes an early and sustained increase in the natural killer cell component of circulating lymphocytes: A clinical trial supporting anti-viral indications. International immunopharmacology, 42.
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