Eos kiss x9

Types.Metaponequadridentata Eguchi, 1998. Three paratypes from East Malaysia (Borneo), Sabah, Poring, Kinabalu were examined. Two paratype workers (SKYC) and one paratype queen (MNHAH).
Source images of the new species for focus stacking were taking using a Canon EOS Kiss X9 digital camera, attached to a Nikon AZ100 stereomicroscope (for worker, queen, and male bodies, excluding male genitalia), and a Nikon Eclipse E600 microscope (for male genitalia). Focus-stacked images were produced using Helicon Focus Pro 7.0.2 (Helicon Soft Ltd., http://www.heliconsoft.com/), and improved with the retouching function of the same software. Colour balance and contrast were adjusted using GIMP 2.8 (The GIMP Development Team, http://www.gimp.org). Two paratype workers of M.quadridentata were imaged on a Dun Inc™ Passport II macrophotography imaging system, using a Canon MP-E 65 mm lens. Focus-stacked images of the two paratypes were produced using Zerene Stacker (Zerene Systems LLC, http://zerenesystems.com/cms/stacker). The final images were further adjusted, annotated, and scale bars added using Adobe Photoshop CS6. All specimens of the new species examined are deposited in the Lee Kong Chian Natural History Museum, under the Zoological Reference Collection (ZRC).
High resolution image plates are available at https://doi.org/10.5061/dryad.9776725

Cephalic capsule, maxillae, mandibles, the forcipular segment and leg-bearing segments were made transparent using lactic acid or the Chelex-TE protocol to examine the anatomy and produce images (Satria et al. 2015 (link)). Cephalic capsule and maxillae of some specimens were mounted in Hoyer’s medium (gum arabic, chloral hydrate, and glycerol) or Euparal. Multi-focused images of these body parts were produced using Helicon Focus Pro 6.7.1 (https://www.heliconsoft.com/helicon-focus-history-of-changes-win/) from a series of source images taken using a Canon EOS Kiss X9 digital camera attached to a Nikon AZ100 microscope and improved using Adobe Photoshop Elements 10. Body parts were then measured directly using an ocular micrometer attached to the microscope or by measuring on the basis of the images using ImageJ software (http://imageJ.nih.gov/ij/). The morphological terminology used in the present study is mainly derived from Bonato et al. (2010) (link). Specimens with fully developed paired gonopods—that is, evidently bi-articulated in males and touching each other in females—were determined to be adults, and those with incompletely developed paired gonopods were determined to be subadults; specimens without gonopods were determined to be juveniles.

NHMWNaturhistorisches Museum Wien, Vienna, AustriaOUMNHOxford University Museum of Natural History, Oxford, UKSKYC Seiki Yamane Collection, Kagoshima, Japan
ZRCZoological Reference Collection, Lee Kong Chian Natural History Museum, SingaporeSource images for focus stacking were taken using a Canon EOS Kiss X9 digital camera, attached to a Nikon AZ100 stereomicroscope (for O.litoralis worker, queen, and male bodies of all species, excluding male genitalia), and a Nikon Eclipse E600 microscope (for male genitalia). Focus-stacked images were produced using Helicon Focus Pro 7.0.2 (Helicon Soft Ltd., http://www.heliconsoft.com/), and improved with the retouching function of the same software. Colour balance and contrast were adjusted using GIMP 2.8 (The GIMP Development Team, http://www.gimp.org).
Workers of O.malignus were imaged with a Dun Inc. Passport II macrophotography imaging system, using a Canon MP-E 65 mm lens; focus-stacked images were produced using Zerene Stacker v1.04 (Zerene Systems LLC, https://zerenesystems.com/cms/stacker). All final images were further adjusted, annotated, and scale bars added using Adobe Photoshop CS6.