Embryos and adult medaka were irradiated with gamma rays emitted by 137Cs (Gammacell 3000Elan, MDS Nordion, Ottawa, Canada) at a dose rate of 7.3 Gy/min at room temperature (RT) in a plastic tub containing water.
Gammacell 3000 elan
Manufactured by Nordion
Sourced in Canada
The Gammacell 3000 Elan is a self-shielded gamma irradiator designed for research applications. The device utilizes a cesium-137 radioactive source to generate gamma radiation for the irradiation of samples.
Automatically generated - may contain errors
Lab products found in correlation
Macs cell separation system, by Miltenyi Biotec (1 mentions)
Lymphoprep, by Axis-Shield (1 mentions)
Rpmi 1640 medium, by Sarstedt (1 mentions)
2 ml reaction tube, by Sarstedt (1 mentions)
T25 flask, by BD (1 mentions)
Matrigel, by Corning (1 mentions)
Cellsens software, by Olympus (1 mentions)
Fitc annexin 5 apoptosis detection kit 1, by BD (1 mentions)
Dld 1, by Korean Cell Line Bank (1 mentions)
Hct116, by Korean Cell Line Bank (1 mentions)
Sw480, by Korean Cell Line Bank (1 mentions)
Ht 29, by Korean Cell Line Bank (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Cisplatin, by Sandoz (1 mentions)
Cetuximab, by Merck Group (1 mentions)
Tissue culture flask, by Corning (1 mentions)
Dialyzed, by Thermo Fisher Scientific (1 mentions)
L lysine, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Difco noble agar, by BD (1 mentions)
22 protocols using gammacell 3000 elan
1
Gamma Irradiation of Medaka Embryos
2
Irradiation of Medaka Embryos
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Embryos at stage 28 (30-somite stage, 64 h after fertilization) were irradiated with γ-ray emitted by 137Cs (10 Gy, Gammacell 3000Elan, MDS Nordion, Ottawa, ON, Canada) at a dose rate of 7.5 Gy/min at room temperature in a plastic tube containing water. Medaka embryos at stage 28 correspond approximately to early fetal stage human embryos (approximately 8–15 weeks postovulation) [65 (link)]. Targeted irradiation to the right hemisphere of OT and the central part of OT of embryonic brain with a collimated microbeam (250 μm diameter) of carbon ions was conducted at the irradiation facility of TIARA (Takasaki Ion Accelerators for Advanced Radiation Application), National Institutes of Quantum Beam Science and Technology (QST) as previously reported [54 (link)]. Irradiation of whole brain of medaka embryo was conducted as reported by Nagata et al. [44 (link)].
3
Spleen-Derived Splenocyte Transfer for Tumor Inoculation
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The animals were killed, and spleens from treated and naïve rats were harvested through abdominal incision. Splenocytes were isolated by density gradient centrifugation with Lymphoprep according to the manufacturer's instruction (Axis-Shield PoC). A fraction of splenocytes was T-cell depleted (TCD) using the MACS cell separation system (Miltenyi Biotec). The splenocytes were incubated with saturating concentrations of biotinylated mAbs, anti-CD5 (OX19) and anti-CD6 (OX52). After antibody-staining, cells were selected using appropriated diluted anti-biotin microbeads. Labeled cells were separated using the magnetic cell separator QuadroMACS. Isolated T cells were collected from the bound fraction, while TCD splenocytes were obtained from the unbound fraction of the LS column.
One day before the adoptive transfer of splenocytes, the rats were conditioned with a total body irradiation (TBI) of 600 cGy from a 137Cs source (Gammacell 3000 Elan, MDS Nordion). Isolated splenocytes were resuspended in PBS and adjusted to a concentration of 30·106 cells/ml or 18·106 cells/ml (TCD). The day after, rats were s.c. inoculated with tumor cells.
One day before the adoptive transfer of splenocytes, the rats were conditioned with a total body irradiation (TBI) of 600 cGy from a 137Cs source (Gammacell 3000 Elan, MDS Nordion). Isolated splenocytes were resuspended in PBS and adjusted to a concentration of 30·106 cells/ml or 18·106 cells/ml (TCD). The day after, rats were s.c. inoculated with tumor cells.
4
Irradiation of Cell Suspensions
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The cells were irradiated in suspension using a blood product irradiator (Gammacell 3000 Elan, Nordion, Ontario, Canada) at single doses of 0, 2, 5, and 10 Gy. The irradiator had a 137Cs source and a cylindrical canister. The dose rate was 3.2 Gy per minute at the center of the canister. Radiation dose homogeneity was estimated at 10% using existing clinical depth-dose data for this irradiator.
5
Gamma Irradiation of Human T Cells
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7.5 × 105 human T cells were transferred in RPMI 1640 medium into a 2 mL reaction tube (Sarstedt, Nümbrecht, Germany). Each reaction tube was treated with a single dose in the range from 2 to 50 Gy as indicated in the respective Figures using the Gammacell® 3000 Elan (Nordion International Inc., Ottawa, ON, Canada).
6
Conditional Reprogramming of Epithelial Cells
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Conditional reprogramming of the brushings was performed as previously described [4] (link). Briefly, 125,000 ex vivo epithelial cells obtained from the brushings were grown in fibronectin pretreated T25 flasks (Falcon, Corning) and the F-medium containing ROCK inhibitor [4] (link). These flasks were pre seeded with 125 000 cells from the murine embryonic fibroblast cell line NIH-3T3, which were irradiated with 3000 cGy γ-radiation (Gammacell® 3000 Elan; MDS Nordion) prior to being added to the flasks in order to establish an irradiated feeder cell layer. Epithelial cells were grown to ∼90% confluence before being trypisinized with LONZA subculture reagent packs (LONZA, Switzerland) and cryogenically frozen at 500,000 cells per vial in 1 mL of freezing media (90% fetal calf serum, 10% dimethyl sulfoxide), labelled as passage zero (P0) cells. Cells were cryogenically frozen in Mr. Frosty freezing containers (Nalgene, ThermoFisher) at -80 °C before being moved to liquid nitrogen for longer term storage.
7
Gamma-ray Irradiation of Zebrafish Embryos
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Embryos at stage 28 (3 dpf) were irradiated with 10 Gy gamma-rays in The University of Tokyo emitted from 137Cs (Gammacell 3000Elan, MDS Nordion, Ottawa, Canada) at a dose rate of 7.3 Gy/min at room temperature in a plastic tub with water. The embryos were exposed to gamma rays with only a limited dose, 10 Gy.
8
Radiation-Induced Endothelial Cell Apoptosis
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Subconfluent HUVECs were exposed to 15 Gy of irradiation at a dose rate of 3.25 Gy/min using a 137Cs γ-ray irradiator (Gammacell 3000 Elan; MDS Nordion, Ontario, Canada). For apoptosis assays, the complete medium was replaced with EBM-2 with 2% FBS, MSC-CM, or PBM-treated MSC-CM. After 24 h of incubation, apoptosis rates were determined by flow cytometry using Annexin V and PI staining (FITC-Annexin V Apoptosis Detection Kit I; BD), according to the manufacturer’s instructions. For tube formation assays, the irradiated HUVECs were detached and re-plated in Matrigel (Corning, Corning, NY, USA)-coated 24-well culture plates using three types of media (the same as those used for apoptosis assays). After 16 h of incubation, the cells were fixed with 4% paraformaldehyde. Four microscopic fields per group were randomly selected to measure total tube length and count the number of branch points using CellSens software (Olympus, Waltham, MA, USA).
9
Radiation Tolerance in Drosophila Larvae
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Radiation tolerance varies greatly according to life stages in D. melanogaster [52 (link)]. We selected the third instar larval stage as the irradiation stage since it is more susceptible to radiation than the adult stage. The eggs were collected from 5- to 7-day-old adult female flies for 8 h on a sterile CSY medium. The feeding third instar larvae were subjected to radiation in a γ-ray irradiation machine at 0.1 Gy (dose rate of 0.67 cGy/min, 137Cs, MDI-KIRMAS 137, Seoul, Korea) or 5 Gy (dose rate of 3.25 Gy/min, 137Cs, Gammacell 3000 Elan, Nordion Inc., Ottawa, ON, Canada). After irradiation, the irradiated and non-irradiated larvae were transferred immediately to new sterile CSY media to exclude secondary effects arising from the microbes in media excreted by Conv flies. Non-irradiated and irradiated flies were maintained contemporaneously under the same conditions at 25 °C.
10
Colon and Rectal Cancer Irradiation
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Colon and rectal cancer cells were irradiated with a 137Cs laboratory γ‐irradiator (Gammacell 3000 Elan; MDS Nordion) at a dose rate of 3.25 Gy/min for the time required to apply a prescribed dose at room temperature.
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