Enzyme linked immunosorbent assay elisa

The morphological shape and formation mechanism of Ag-nanostructure materials were characterized by using Transmission Electron Microscope (TEM, JEM-2100, Akishima, Tokyo, Japan) and High-resolution Transmission Electron Microscope (HR-TEM, FEI Talos F200C at 200 kV, Tokyo, Japan) and scanning electron microscopy (SEM, JSM-6700F, Akishima, Tokyo, Japan). The XRD analysis was performed with a Bruker D8 Advance X-ray diffractometer equipped with a Cu Ka radiation source (Karlsruhe, Germany). The measurements of optical properties were conducted by using a PerkinElmer UV/VIS Spectrometer Lambda25, manufactured by PerkinElmer, Ayer Rajah Crescent, Singapore Pte Ltd., and UV-2450 UV-vis Shimadzu spectrophotometer, Kyoto, Japan. Meanwhile, the hydrodynamic size and zeta potential were also measured by dynamic light scatting (DLS) using a Nano-ZS ZEN3600 Malvern Instruments, Worcestershire, UK. Also, the measurements of the cell viability were carried out by using Enzyme-Linked Immunosorbent Assay (ELISA, Thermo Fisher Scientific, Waltham, MA, USA) at 570 nm wavelength.

Sleep quality was examined with the Pittsburgh Sleep Quality Index (PSQI), comprising 19 items that identify seven core elements of sleep: sleep quality, how long subjects take to fall asleep, percentage of time in bed, sleep duration, sleep disturbances, medications for sleep, and daytime dysfunction. Global scores of ≥5 indicate poorsleep and <5 good sleep with a sensitivity of 98.7 and specificity of 84.4 for the diagnosis of sleep quality. Total cholesterol (TC), triglycerides (TG), and high-density lipoprotein (HDL) were examined with a one-step enzymatic method (Erba Mannheim, London, UK) with a minimum detection limit of 0.1 mg/L. Low-density lipoprotein (LDL) was evaluated with the Friedewald equation. An Enzyme Linked Immunosorbent Assay (ELISA) was used to determine plasma levels of IL-6, IL-1β, TNF-α, and hsCRP(ThermoFisher Scientific, Waltham, MA, USA), on a microplate reader (Biotek Instruments Inc., Winooski, VT, USA). Diagnostic sensitivities of the kits were <1.0 pg/mL, 0.35 pg/mL, 1.75 pg/mL, and 9.38 pg/mL for IL-6, IL-1β, TNF-α, and hsCRP, respectively. The coefficients of variation for inter- and intra-assay analyses were less than 5% for all these assays.

Serum levels of total IgG and IgM were detected with Enzyme Linked Immunosorbent Assay (ELISA) from ThermoFischerScientific. Serum was diluted by 1:10,000 (IgM) or 1:50,000 (IgG).