Plasma levels of the immune activation markers sCD14, sCD163, TNF receptor (TNFR)-I, and TNFR-II were measured using Quantikine enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems). Levels of Lp-PLA2 were measured by a quantitative ELISA (Plac Test; DiaDexus, South San Francisco, CA). Endothelial activation markers VCAM-1, ICAM-1, fractalkine (CX3CL1) (R&D Systems), and ICAM-2 (Abcam, Cambridge, MA) were measured by ELISA. Oxidized LDL was measured by ELISA (Mercodia, Uppsala, Sweden).
Fractalkine cx3cl1
Manufactured by R&D Systems
Sourced in United States
Fractalkine (CX3CL1) is a chemokine protein produced by various cell types, including endothelial cells, neurons, and epithelial cells. It functions as a chemoattractant and adhesion molecule, playing a role in the recruitment and migration of immune cells, particularly monocytes and lymphocytes.
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Lab products found in correlation
2 protocols using fractalkine cx3cl1
1
Immune and Endothelial Activation Markers
2
Quantification of Inflammatory Markers in 2K1C Rat Model
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Peripheral blood was collected by cardiac puncture at 1 and 8 weeks post-2K1C surgery and T. cruzi infection. After centrifugation at 3000 g for 10 min at 4°C, serum was collected and Fractalkine/CX3CL1 (R&D Systems, USA) and TNF (PeproTech, USA) levels were evaluated by immunoassay (ELISA) using a kit as per the manufacturer's instructions. Briefly, rat serum samples and the protein of interest (or standard) were added to a 96-well plate that was pre-coated with a monoclonal antibody specific for rat Fractalkine/CX3CL1 or TNF. After 1 h of incubation at 20°C, plates were washed and incubated with detection antibody for 30 min. The plates were washed again and incubated with 3,3′,5,5′-tetramethylbenzidine (TMB) substrate for 15 min. After the reaction stopped, the absorbance at 450 nm was measured using a microplate reader (Bio-Rad, USA).
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