Lc ms grade acetonitrile

Manufactured by Merck Group

Sourced in Germany, United States, Italy, United Kingdom, France, Poland, China, Switzerland, Spain, Malaysia, India

LC-MS grade acetonitrile is a high-purity solvent commonly used in liquid chromatography-mass spectrometry (LC-MS) applications. It is a colorless, volatile liquid with a low boiling point. The high level of purity ensures minimal interference with analytical measurements.

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Lab products found in correlation

Formic acid, by Merck Group (92 mentions) Methanol, by Merck Group (75 mentions) Milli q system, by Merck Group (21 mentions) Lc ms grade formic acid, by Merck Group (19 mentions) Ammonium acetate, by Merck Group (15 mentions) Lc ms grade methanol, by Merck Group (15 mentions) Formic acid, by Thermo Fisher Scientific (15 mentions) Ammonium formate, by Merck Group (13 mentions) Hplc grade methanol, by Merck Group (12 mentions) Lc ms grade water, by Merck Group (11 mentions) Dimethyl sulfoxide (dmso), by Merck Group (11 mentions) Milli q water purification system, by Merck Group (11 mentions) Hydrochloric acid (hcl), by Merck Group (9 mentions) Quercetin, by Merck Group (9 mentions) Acetic acid, by Merck Group (9 mentions) Sodium hydroxide (naoh), by Merck Group (8 mentions) Ethyl acetate, by Merck Group (8 mentions) Ms grade formic acid, by Merck Group (8 mentions) Milli q purification system, by Merck Group (8 mentions) Ammonium bicarbonate, by Merck Group (8 mentions)

Close spelling variants of this product

LC-MS grades of acetonitrile LC-grade acetonitrile MS-grade acetonitrile LC-MS acetonitrile LC-MS grade Acetonitrile

279 protocols using lc ms grade acetonitrile

Quantification of Neonatal Plasma Drugs

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Ampicillin, caffeine citrate, fluconazole, midazolam, α-hydroxymidazolam, vancomycin hydrochloride, and the internal standards (IS) caffeine-¹³C3 and midazolam-D4 maleate, LC–MS grade acetonitrile, water, and formic acid were purchased from Sigma Aldrich (St. Louis, MO, USA). All other chemicals and agents were of the highest analytical grade commercially available.
Blank human plasma was purchased from Innovative Research (Novi, Michigan, USA). Blood samples were obtained from three preterm and three full-term neonates treated in the NICU of the University of Michigan Hospital. The study was approved by the Institutional Review Board at the University of Michigan. Plasma was separated from the whole blood after centrifugation and stored at −80°C until use.

Xiao J., Shi J., Li R., Her L., Wang X., Li J., Sorensen M.J., Bhatt-Mehta V, & Zhu H.J. (2021). Developing a SWATH capillary LC-MS/MS method for simultaneous therapeutic drug monitoring and untargeted metabolomics analysis of neonatal plasma. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1179, 122865.

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MALDI-TOF Mass Spectrometry Protocol

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≥ 99.8% ethanol, ≥ 98% (TLC-grade) α-cyano-4-hydroxycinnamic acid (HCCA) matrix, LC–MS-grade acetonitrile, and 99% ReagentPlus^®-grade TFA were purchased from Sigma (Gillingham, UK). CHROMASOLV™ LC–MS-grade water was purchased from Fluka (Loughborough, UK).

Reeve M.A, & Pollard K.M. (2019). Discrimination between regional biotypes of Impatiens glandulifera using a simple MALDI-TOF MS-based method for use with seeds. Plant Methods, 15, 25.

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Phospholipid-Based Antioxidant Extraction

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Phospholipon90G (P90G; phosphatidylcholine content ≥ 94%) was purchased from Lipoid GmbH (Ludwigshafen, Germany). Propylene glycol (PG) were purchased from Galeno Srl (Comeana, Prato, Italy). LC-MS grade acetonitrile, formic acid, methanol, 85% w/w phosphoric acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tris(pyridin-2-yl)-1,3,5-triazine (TPTZ), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), dimethyl sulphoxide (DMSO), and all the other reagents, if not otherwise specified, were purchased from Sigma-Aldrich/Merck (Milan, Italy). Standards of 5-O-caffeoylquinic (chlorogenic acid), 1,3-dicaffeoylquinic acid (cynarin), 3,5-dicaffeoylquinic acid (isochlorogenic acid A), 3,4-dicaffeoylquinic acid (isochlorogenic acid B), 4,5-dicaffeoylquinic acid (isochlorogenic acid C), ferulic acid, apigenin, hispidulin, and luteolin were purchased from Extrasynthese (Genay, France) and TransMIT (Giessen, Germany). Ultrapure water (18 MΩ·cm) was obtained with a Milli-Q Advantage A10 System apparatus (Millipore, Milan, Italy).

Caddeo C., Tuberoso C.I., Floris S., Masala V., Sanna C, & Pintus F. (2023). A Nanotechnological Approach to Exploit and Enhance the Bioactivity of an Extract from Onopordum illyricum L. Leaves. Plants, 12(7), 1453.

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Cryopreserved Hepatocyte Protocol

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Cryopreserved human hepatocytes, human liver microsomes, thawing and incubation media and NADPH regenerating system solutions were purchased from BioreclamationIVT (Baltimore, MD). STS-135 was obtained from Cayman Chemicals (Ann Arbor, MI). LC-MS-grade acetonitrile and water were supplied by Sigma-Aldrich (St. Louis, MO).

Gandhi A.S., Wohlfarth A., Zhu M., Pang S., Castaneto M., Scheidweiler K.B, & Huestis M.A. (2014). High-resolution mass spectrometric metabolite profiling of a novel synthetic designer drug, N-(adamantan-1-yl)-1-(5-fluoropentyl)-1H-indole-3-carboxamide (STS-135) using cryopreserved human hepatocytes and assessment of metabolic stability with human liver microsomes. Drug testing and analysis, 7(3), 187-198.

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HPLC-MS Analysis of Chloramphenicol

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LC/MS-grade acetonitrile and LC/MS-grade methanol were from Sigma-Aldrich. Formic for LC/MS was from Sigma-Aldrich. HPLC grade water was obtained from HLP 5 (HYDROLAB Poland) apparatus and was filtered through the 0.2 μm filter before the use. Chloramphenicol (≥98%, TLC) was from Sigma. Harpagide analytical standard (≥98%, HPLC) was from Sigma.

Skrzypczak-Pietraszek E., Reiss K., Żmudzki P, & Pietraszek J. (2018). Enhanced accumulation of harpagide and 8-O-acetyl-harpagide in Melittis melissophyllum L. agitated shoot cultures analyzed by UPLC-MS/MS. PLoS ONE, 13(8), e0202556.

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Cryopreserved Hepatocyte Incubation Protocol

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Cryopreserved human hepatocytes, HLM, thawing and incubation media were
purchased from BioreclamationIVT (Baltimore, MD, USA). NADPH regenerating system
solutions A and B were purchased from Corning, Inc. (part #s 451220 and
451200, respectively). AH-7921 (97.94±1.04% purity) was kindly
provided by the Drug Enforcement Administration Special Testing and Research
Laboratory (Dulles, VA, USA). Liquid chromatography-mass spectrometry
(LC-MS)grade acetonitrile and Trypan Blue were purchased from Sigma-Aldrich (St.
Louis, MO, USA), while potassium phosphate, glacial acetic acid and formic acid
(LC-MS grade) were obtained from Fisher Scientific (Waltham, MA, USA). APCI
positive calibration solution was from SCIEX (part # 4460131, Redwood
City, CA, USA). Water was purified in house with an ELGA Purelab Ultra Analytic
purifier (Siemens Water Technologies, Lowell, MA, USA).

Wohlfarth A., Scheidweiler K.B., Pang S., Zhu M., Castaneto M., Kronstrand R, & Huestis M.A. (2015). Metabolic characterization of AH-7921, a synthetic opioid designer drug: in vitro metabolic stability assessment and metabolite identification, evaluation of in silico prediction, and in vivo confirmation. Drug testing and analysis, 8(8), 779-791.

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Comprehensive Biochemical Analysis Protocol

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All reagents and chemicals were analytical grades. Trizma base, hydrochloric acid, 5,5′-dithiobis-(2-nitrobenzoic acid), physostigmine, acetylthiocholine iodide, and acetylcholinesterase were purchased from Merck (Darmstadt, Germany). The COX-2 Inhibitor Screening Kit was purchased from Abcam (Cambridge, UK). LC-MS grade acetonitrile, LC-MS grade m ethanol, ethanol, dimethyl sulfoxide, catechin, sodium nitrite, aluminum chloride, sodium hydroxide, gallic acid, 2,2-difenil-1-picrilhidrazil (DPPH), iron (III) chloride hexahydrate, and formic acid used were purchased from Sigma-Aldrich (St. Louis, MO, USA). Folin-Ciocalteu reagent, sodium acetate solution, sodium carbonate, and 2,4,6-tripyridyl-s-triazine (TPTZ) were purchased from Thermo Fisher (Waltham, MA, USA). Finally, 2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) was purchased from Roche (Basel, Switzerland), and the water used was Milli-Q type obtained from Millipore purification equipment (Billerica, MA, USA).

Romero-Márquez J.M., Navarro-Hortal M.D., Forbes-Hernández T.Y., Varela-López A., Puentes J.G., Pino-García R.D., Sánchez-González C., Elio I., Battino M., García R., Sánchez S, & Quiles J.L. (2023). Exploring the Antioxidant, Neuroprotective, and Anti-Inflammatory Potential of Olive Leaf Extracts from Spain, Portugal, Greece, and Italy. Antioxidants, 12(8), 1538.

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Quantitative Analysis of Taxane Drugs

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nab-P and mouse-specific nab-P (m-nab-P) were supplied by Celgene Corporation (Summit, NJ, USA), and pac-T was purchased from the University of Michigan Hospital (Hospira Inc, Lake Forest, IL, USA). Paclitaxel and docetaxel powder were procured from Thermo Fisher Scientific (Waltham, MA, USA). Liquid chromatography–mass spectrometry (LC–MS) grade acetonitrile was purchased from Sigma-Aldrich (St. Louis, MO, USA). Formic acid (98%; LC–MS grade) was obtained from Fluka (Morris Plains, NJ, USA). A Milli-Q water system from Millipore (Bedford, MA, USA) was used to obtain ultrapure deionized water.

Li F., Yuan H., Zhang H., He M., Liao J., Chen N., Li Y., Zhou S., Palmisano M., Yu A., Pai M, & Sun D. (2019). Neonatal Fc Receptor (FcRn) Enhances Tissue Distribution and Prevents Excretion of nab-Paclitaxel. Molecular pharmaceutics, 16(6), 2385-2393.

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Synthesis and Characterization of Oncrasin Compounds

Cited 2 times

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Oncrasin-266, Oncrasin-72, NSC-741980, and internal standard (IS, 1-(3-chlorobenzyl)-1H-indole) were synthesized as described previously [5 (link), 6 (link)]. These compounds were 96%, 97.5%, 99%, and 99% pure, respectively. Purity and molecular weight was confirmed by HPLC/MS/UV₂₃₀. Formic acid, LC-MS grade acetonitrile and water were purchased from Sigma-Aldrich (St. Louis, MO).

White L., Wu S., Ma J., Fang B, & Liang D. (2016). Simultaneous Determination and Validation of Oncrasin-266 and its Metabolites by HPLC-MS/MS: Application to a Pharmacokinetic Study. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1033-1034, 106-111.

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LC-MS-based Metabolite Analysis

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LC/MS-grade water and LC/MS-grade methanol (CAS 67-56-1) were from Honeywell Burdick & Jackson (Morristown, NJ). LC/MS-grade acetonitrile (CAS 75-05-8) and ammonium acetate (CAS 631-61-8) were from Sigma-Aldrich (St. Louis, MO). LC/MS internal standards included MOPS (CAS 1132-61-2), HEPES (CAS 7365-45-9), 3,6-dihydroxy-4-methylpyridazine (CAS 5754-18-7), 4-(3,3-dimethyl-ureido)benzoic acid (CAS 91880-51-2), d₅-benzoic acid (CAS 1079-02-3) and 9-anthracenecarboxylic acid (CAS 723-62-6) from Sigma-Aldrich.

Swenson T.L., Karaoz U., Swenson J.M., Bowen B.P, & Northen T.R. (2018). Linking soil biology and chemistry in biological soil crust using isolate exometabolomics. Nature Communications, 9, 19.

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