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B0357

Manufactured by Agilent Technologies
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The B0357 is a compact and versatile laboratory instrument designed for a wide range of applications. It features accurate and reliable performance, making it a valuable tool for various research and analytical tasks.

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Lab products found in correlation

Ab79823, by Agilent Technologies (2 mentions) Dako mounting reagent, by Agilent Technologies (2 mentions) Clone 17a2, by Thermo Fisher Scientific (2 mentions) Clone rm4 5, by Thermo Fisher Scientific (2 mentions) γδ tcr, by BD (2 mentions) Clone gl3, by BioLegend (2 mentions) Cd11b, by Thermo Fisher Scientific (2 mentions) Clone m1 70, by Cambridge Bioscience (2 mentions) Cd11c, by Cambridge Bioscience (2 mentions) Efluor 660, by Thermo Fisher Scientific (1 mentions) Clone pk136, by Thermo Fisher Scientific (1 mentions) Clone bm8, by Thermo Fisher Scientific (1 mentions)

2 protocols using b0357

1

Immunofluorescent Staining of Tissue Sections

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Frozen sections were fixed in acetone/methanol (1:1) or 4% paraformaldehyde/PBS pH 7.4 and blocked with 2% BSA, 0.02% fish skin gelatin and 10% goat serum for 1 h in PBS at room temperature. Paraffin sections were subjected to heat-mediated antigen retrieval (citrate buffer, pH6). When fluorochrome-conjugated primary antibodies were used, sections were incubated overnight at 4 °C in antibody solution containing 4',6-diamidino-2-phenylindole, washed in PBS and then mounted using the DAKO mounting reagent (DAKO). In the case of the unconjugated primary antibodies (HMGB1, Abcam, ab79823; anti-Escherichia coli antibody, DAKO, B0357), incubation was overnight at 4 °C followed by 2 h incubation at room temperature in secondary antibody (AlexaFluor 555 goat-anti-rabbit) containing 4,6-diamidino-2-phenylindole. Alexafluor 647-, 660- or 488-conjugated antibodies to CD3 (BioLegend, clone 17.A2), CD4 (eBioscience, clone RM4-5), F4/80 (eBioscience, clone BM8), CD19 (eBioscience, eFluor 660), CD11b (eBioscience, clone M1/70), CD11c (Cambridge Bioscience), γδ TCR (BD Pharmingen, clone GL3), NK1.1 (BioLegend, clone PK136) and CD207 (eBioscience, eFluor 660) were used.
Hoste E., Arwert E.N., Lal R., South A.P., Salas-Alanis J.C., Murrell D.F., Donati G, & Watt F.M. (2015). Innate sensing of microbial products promotes wound-induced skin cancer. Nature Communications, 6, 5932.
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2

Multiparameter Immunofluorescence Staining

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Frozen sections were fixed in acetone:methanol (1:1) or 4% paraformaldehyde/PBS pH 7.4 and blocked with 2% BSA, 0.02% fish skin gelatin and 10% goat serum for 1 hour in PBS at room temperature. Paraffin sections were subjected to heat-mediated antigen retrieval (citrate buffer, pH6). When fluorochrome-conjugated primary antibodies were used, sections were incubated overnight at 4°C in antibody solution containing DAPI, washed in PBS and then mounted using the DAKO mounting reagent (DAKO). In the case of the unconjugated primary antibodies (HMGB1, Abcam, ab79823; anti-Escherichia coli antibody, DAKO, B0357) incubation was overnight at 4°C followed by 2h incubation at room temperature in secondary antibody (AlexaFluor 555 goat-anti-rabbit) containing DAPI. Alexafluor 647, 660 or 488 conjugated antibodies to CD3 (BioLegend, clone 17.A2), CD4 (eBioscience, clone RM4-5), F4/80 (eBioscience, clone BM8), CD19 (eBioscience, eFluor 660), CD11b (eBioscience, clone M1/70), CD11c (Cambridge Bioscience), γδ TCR (BDPharmingen, clone GL3), NK1.1 (BioLegend, clone PK136) and CD207 (eBioscience,eFluor 660) were used.
Hoste E., Arwert E.N., Lal R., South A.P., Salas-Alanis J.C., Murrell D.F., Donati G, & Watt F.M. (2015). Innate sensing of microbial products promotes wound-induced skin cancer. Nature Communications, 6, 5932.
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