For starvation experiments, 3-week-old C57BL/6 J mice were purchased from Central Lab Animal Inc. and maintained on a normal diet for 5 weeks. The mice were randomly allocated to the following three experimental groups: control group (n = 4), fed ad libitum for 24 h; starvation group (n = 4), starved for 24 h; refed group (n = 5), starved for 24 h followed by 6 h of feeding. The mice were then sacrificed, and the soleus muscles were dissected. The muscle tissue was homogenized and sonicated with a Bioruptor (Diagenode) for 10 min and centrifuged. All animal experiments were approved by the Institutional Animal Care and Use Committee of Sungkyunkwan University (approval no. SKKUIACUC2019-03-22-1).
C57bl 6j mice
Manufactured by Central Lab Animal
Sourced in Cameroon, United States
The C57BL/6J mice are a commonly used inbred mouse strain. They are a well-characterized genetic model and are widely used in biomedical research.
Automatically generated - may contain errors
Lab products found in correlation
High fat diet (hfd), by Research Diets (3 mentions)
Bioruptor, by Diagenode (1 mentions)
Ain 93g diet, by Research Diets (1 mentions)
Oxymax lab animal monitoring system, by Columbus Instruments (1 mentions)
Iec 6, by Korean Cell Line Bank (1 mentions)
Gammacell 3000 elan, by Best Theratronics (1 mentions)
B6 129 mertktm1grl j, by Jackson ImmunoResearch (1 mentions)
D12492, by Jackson ImmunoResearch (1 mentions)
Rodent diet d12492, by Research Diets (1 mentions)
Td 06414, by Inotiv (1 mentions)
2018s teklad global 18 protein rodent diet, by Inotiv (1 mentions)
L 8900 amino acid analyzer, by Hitachi (1 mentions)
Avertin, by Merck Group (1 mentions)
Leukocyte acid phosphatase staining kit, by Merck Group (1 mentions)
Red blood cell lysis buffer, by Merck Group (1 mentions)
Accu chek, by Roche (1 mentions)
31 protocols using c57bl 6j mice
1
Starvation Effects on Soleus Muscle
2
Dietary Effects on C57BL/6J Mice
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Forty-six-week-old male C57BL/6J mice were purchased from Central Lab Animal Inc. (Seoul, Korea). The mice were fed a solid diet (Research Diets, New Brunswick, NJ, USA) for a week prior to the experiment to acclimate them to the environment, after which, they were divided into four groups of ten using a randomized block design and were reared for 10 weeks. Table 1 presents the experimental diet. The experimental groups were divided into the normal diet (ND) group, high-fat and -cholesterol diet (HFCD) group, high-fat and -cholesterol diet with 5% RB (HFCD-RB) group, and high-fat and -cholesterol diet with 5% fermented RB (HFCD-FRB) group. The HFCD consisted of 20% fat (41% of calories from fat) and 1.25% cholesterol based on the AIN-93G diet (D12451, Research Diets Inc., Brunswick, NJ, USA). Body weight was measured once a week during the rearing period, and food intake was measured at the same time every day by subtracting the amount left over from the amount served. Water and food were consumed ad libitum. The temperature and humidity were maintained at 18 ± 2 °C and 50–60%, respectively, with a 12 h light–dark cycle (08:00–20:00).
3
FGF21 Mutant Fusion Protein Reduces Obesity
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Example 4
The body weight-reduction effect of DFD18, an FGF21 mutant fusion protein, was evaluated in diet-induced obese mice. For the diet-induced obesity model, C57BL/6J mice were purchased from Central Lab. Animal Inc. and fed on a high-fat diet containing 60 kcal % fat (Research diet) for 8 to 12 weeks. The mice were divided into groups (n=8/group) in order to have a similar mean value of body weight one day before the drug treatment (Day 0), and then 30 nmol/kg of samples were subcutaneously administered once. Subsequently, a single subcutaneous administration at a dose of 30 nmol/kg was performed, followed by the observation of a change in the body weight as compared to phosphate buffered saline (PBS) as a solvent.
For changes in body weight over time in the diet-induced obesity mouse model following single administration of 30 nmol/kg DFD18, it was confirmed that the weight-reducing effect was continuing by the 10th day after the administration, and the maximum weight reduction (about 18%) was at the 11th day after the administration, which was maintained by the 14th day (
4
FGF21 Mutant Fusion Protein for Obesity
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Example 6
The body weight-reduction effect of DFD18, an FGF21 mutant fusion protein, was evaluated in diet-induced obese mice. For the diet-induced obesity model, C57BL/6J mice were purchased from Central Lab. Animal Inc. and fed on a high-fat diet containing 60 kcal % fat (Research diet) for 8 to 12 weeks. The mice were partitioned into groups (n=8/group) in order to have a similar mean value of body weight one day before the drug treatment (Day 0), and then 30 nmol/kg of samples were subcutaneously administered once. The changes in body weights were compared with the group treated with vehicle (PBS).
For changes in body weight over time in the diet-induced obesity mouse model following single administration of 30 nmol/kg DFD18, it was confirmed that the weight-reducing effect was continuing by the 10th day after the administration, and the maximum weight reduction (about 18%) was at the 11th day after the administration, which was maintained by the 14th day (
5
STZ-Induced Diabetes in Mice: BGE and RGE Effects
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All animal work was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Committee on the Ethics of Animal Experiments of Korea University (Permit Number: KUIACUC-2015-127). A timeline of the study is shown in Fig 1A . Male 8-week-old C57BL/6J mice (Central Lab. Animal Inc., Seoul, Korea) were housed with a 12-h light-dark cycle. After an adaptation period of 1 week, mice were divided into 6 treatment groups as follows: 1) non-STZ + vehicle, 2) STZ + vehicle, 3) STZ + BGE50, 4) STZ + BGE100, 5) STZ + BGE200 and 6) STZ + RGE200. All compounds were administered by gavage using an esophageal cannula once daily for 5 weeks with vehicle (normal saline), BGE 50, 100 or 200 mg/kg or RGE 200 mg/kg in saline. Induction of diabetes was initiated after 2 weeks of oral administration: mice were treated intraperitoneally for 5 consecutive days with either 50 mg/kg STZ (dissolved in 50 mM citrate buffer, pH 4.5) or citrate buffer alone. All mice received a normal chow diet with water ad libitum during the experimental period. At the end of the study, the mice were euthanized by an overdose of avertin, and blood was collected by cardiac puncture.
6
FGF21 Mutant Fusion Protein for Obesity
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Example 6
The body weight-reduction effect of DFD18, an FGF21 mutant fusion protein, was evaluated in diet-induced obese mice. For the diet-induced obesity model, C57BL/6J mice were purchased from Central Lab. Animal Inc. and fed on a high-fat diet containing 60 kcal % fat (Research diet) for 8 to 12 weeks. The mice were partitioned into groups (n=8/group) in order to have a similar mean value of body weight one day before the drug treatment (Day 0), and then 30 nmol/kg of samples were subcutaneously administered once. The changes in body weights were compared with the group treated with vehicle (PBS).
For changes in body weight over time in the diet-induced obesity mouse model following single administration of 30 nmol/kg DFD18, it was confirmed that the weight-reducing effect was continuing by the 10th day after the administration, and the maximum weight reduction (about 18%) was at the 11th day after the administration, which was maintained by the 14 day (
7
Protective Effects of EEDK on PONC-Induced Retinal Damage
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All animal studies were conducted in a pathogen-free barrier zone at the KIST Gangneung Institute and were performed in accordance with the procedure outlined in the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research. Procedures used in this study were approved by the Animal Care and Use Committee of KIST (Approval No. 2014-011).
In the present study, male C57BL/6 J mice weighing between 20–25 g (6 weeks of age, Central Lab. Animal Inc., Seoul, Korea) were used to study the protective effects of EEDK on partial optic nerve crush (PONC)-induced retinal damage. The mice were acclimated for 1 week, caged in groups of 8 mice, and had access to animal chow and water ad libitum. The mice were housed at 23 ± 0.5 °C and 10% humidity, with a 12-h light-dark cycle.
In the present study, male C57BL/6 J mice weighing between 20–25 g (6 weeks of age, Central Lab. Animal Inc., Seoul, Korea) were used to study the protective effects of EEDK on partial optic nerve crush (PONC)-induced retinal damage. The mice were acclimated for 1 week, caged in groups of 8 mice, and had access to animal chow and water ad libitum. The mice were housed at 23 ± 0.5 °C and 10% humidity, with a 12-h light-dark cycle.
8
C57BL/6J Mouse Housing Protocol
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C57BL/6J mice were purchased from Central Lab. Animal Inc. (Seoul, Korea). Care, use and treatment of all animals in this study were in strict agreement with the ARVO statement for the Use of Animals in Ophthalmic and Vision Research. C57BL/6J mice were kept in standard 12-hr dark–light cycle and ∼23°C room temperature.
9
Metabolic Profiling of Nrf2 KO Mice
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All animal studies were carried out in accordance with the guidelines of the Animal Research Committee (SKKUIACUC2020- 08-21-1) of Sungkyunkwan University. Seven weeks old male C57BL/6J mice were purchased from Central Lab Animal Inc (Seoul, KoreA). and housed in rooms at ambient temperature. The Nrf2 KO mice were previously described (35 (link)). Whole-body energy metabolism was measured using the Columbus Instruments Oxymax Lab Animal Monitoring System. The mice were placed in metabolic cages and acclimated in the metabolic chambers for one day before measuring O2 consumption.
10
Irradiation of Murine Intestinal Cells
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Rat intestinal epithelial cell line IEC-6 was obtained from the Korean Cell Line Bank (Seoul, South Korea) and maintained in DMEM with 10% FBS and 1% penicillin–streptomycin. Human recombinant proteins were purchased from Prospec (East Brunswick, NJ, USA). A total of 8–9-week-old male C57BL/6 J mice (Central Lab Animal Inc., Seoul, South Korea) were housed at room temperature under 12-h light–dark cycle and allowed access to water and chow ad libitum. All animal experiments were approved by the Institutional Animal Care and Use Committee at the Korea Advanced Institute of Science and Technology (KA2015-35). Mice and cells were irradiated at a dose rate of 2.16 Gy/min using a Cs-137 irradiator (Gammacell 3000 Elan, Best Theratronics, Ottawa, ON, Canada). Irradiation was performed without anesthesia on mice held in a 50-ml conical tube on a rotating turntable. Dosimetric quality assurance was performed using nanoDots (Al2O3:C) optically stimulated luminescence dosimeters (Landauer, Glenwood, IL, USA), which were read using a MicroStar OSL reader (Landauer, Glenwood, IL, USA).
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