Jem 1220

Manufactured by JEOL

Sourced in Japan, United States

The JEM-1220 is a transmission electron microscope (TEM) manufactured by JEOL. It is designed to provide high-resolution imaging and analysis capabilities for a wide range of materials and applications. The JEM-1220 features an accelerating voltage of up to 120 kV and is capable of achieving a spatial resolution of 0.23 nanometers.

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Lab products found in correlation

Morada 11 megapixel camera, by Olympus (5 mentions) Formvar coated copper grids, by Agar Scientific (3 mentions) S 4800, by Hitachi (3 mentions) Morada 11 megapixels camera, by Olympus (2 mentions) Zetasizer nano zs model zen3500, by Malvern Panalytical (2 mentions) Zetasizer nano s90, by Malvern Panalytical (2 mentions) Jem 7100f, by JEOL (2 mentions) Em uc6, by Leica camera (2 mentions) Zetasizer nano zs90, by Malvern Panalytical (2 mentions) Ultracut uct, by Leica camera (1 mentions) Carbon, by Electron Microscopy Sciences (1 mentions) Fluka epoxy embedding medium kit, by Merck Group (1 mentions) Lacey carbon grid, by Electron Microscopy Sciences (1 mentions) Milli q water system, by Merck Group (1 mentions) Nicolet 8700 ftir, by Thermo Fisher Scientific (1 mentions) Ultracut uct model ultramicrotome, by Leica camera (1 mentions) Reichert supernova, by Leica Microsystems (1 mentions) Quetol 812, by Nisshin EM (1 mentions) Ht7700, by Hitachi (1 mentions) Microsuite 5 software, by Olympus (1 mentions)

85 protocols using jem 1220

Ultrastructural Analysis of Dermal Fibrils

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Samples (0.5 × 0.5 × 0.5 mm) were fixed in 3.0% glutaraldehyde in 0.1 M phosphate buffer
(pH 7.4) for 2 hr at room temperature. The samples were then post-fixed in 1.0% osmium
tetroxide in 0.1 M phosphate buffer for 1 hr at room temperature. Thereafter, samples were
washed with distilled water, dehydrated in graded ethanol series and embedded in Quetol
812 (Nissin EM, Tokyo, Japan). Sections of approximately 60 nm in thickness were cut with
a Reichert Supernova system (Leica, Vienna, Austria) equipped with a diamond knife.
Sections were mounted on a copper grid and consecutively stained with 0.2% tannic acid +
10% ethanol in water for 15 min, 1.0% uranyl acetate for 5 min and 1.0% lead citrate for
10 sec. A TEM (JEM-1220; JEOL, Tokyo, Japan) at an accelerating voltage of 80 kV was used
for the investigation. Five hundred fibrils randomly selected from bottom of dermal layer
in photographs of each skin sample were measured by Image J, and the average value was
obtained.

HIROSE T., OGURA T., TANAKA K., MINAGUCHI J., YAMAUCHI T., FUKADA T., KOYAMA Y.I, & TAKEHANA K. (2015). Comparative study of dermal components and plasma TGF-β1 levels in Slc39a13/Zip13-KO mice. The Journal of Veterinary Medical Science, 77(11), 1385-1389.

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Quantifying Cellular Autophagosomes by TEM

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Preparation of pelleted sample: 2×10⁶ cells on culture dishes were rinsed with 0.1M Sorensen's Phosphate Buffer pH 7.4 three times and then fixed using 2.5% glutaraldehyde in 0.1M Sorensen's Phosphate Buffer pH 7.4 for 30 minutes. The cells were then scraped off and transferred to a 1.5 ml Eppendorf tubes and spun down in a microcentrofuge at maximal speed for 10 minutes. The cells were kept at 4°C continuously in fixation solution for 48 hours. The cells were then placed in post-fixative 1% Osmium Tetroxide and dehydrated in a series of ascending ethanol. The cells was then embedded and cured in Epoxy Resin Lx112.
Ultra microtomy with Leica Ultra-cut UCT: Thin sections of the embedded cells were cut 80nm placed on 200 mesh copper standard grids and stained with uranyl acetate and Reynolds lead citrate. The cells were then scanned on a Jeol Jem 1220 Transmission electron microscope. Digital images were acquired with Gatan Erlangshen ES 10000 W Model 785 digital camera with digital micrograph software program 1.7.1 Digital Micrograph DM.
Quantification of autophagosomes: The whole grid square was systematically scanned under the microscope for the presence of early and late autophagic vacuoles. The number of vacuoles per cell area was calculated by dividing the number of vacuoles in the grid square with the cell area in the same grid square.

Duan L., Perez R.E., Davaadelger B., Dedkova E.N., Blatter L.A, & Maki C.G. (2015). p53-regulated autophagy is controlled by glycolysis and determines cell fate. Oncotarget, 6(27), 23135-23156.

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Visualizing Drug Delivery Micelle Morphology

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The morphology of
each DM was visualized using transmission electron microscopy (TEM,
JEM-1220, JEOL Ltd., Japan). A drop (5 μL) of each micellar
suspension (0.2 mg/mL) was placed on a 300 mesh copper grid coated
with carbon (Electron Microscopy Sciences, Hatfield, PA). All samples
were stained with a drop (5 μL) of 2% phosphotungstic acid (pH
7) and dried in a desiccator at room temperature for 1 day before
observed using TEM as described earlier.^{19 (link)}

Hsu H.J., Sen S., Pearson R.M., Uddin S., Král P, & Hong S. (2014). Poly(ethylene glycol) Corona Chain Length Controls End-Group-Dependent Cell Interactions of Dendron Micelles. Macromolecules, 47(19), 6911-6918.

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Immunoelectron Microscopy of CXCL14

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Immunoelectron microscopy was performed as described in our previous studies [23 (link), 38 (link), 54 (link)]. For the pre-embedding method, portions of the stomach and rectum dissected from similarly perfused mice were used. Small blocks of the fixed samples were cut into 20 μm-thick sections and processed; CXCL14 immunoreactivity was detected by DAB reaction. The sections were then fixed in 1% OsO₄ for 1 hr at room temperature, dehydrated and embedded in Quetol 812 (Nissin EM, Tokyo, Japan). Subsequently, ultrathin sections were obtained and mounted on nickel grids. After staining with uranyl acetate and lead citrate for 1 min each, the sections were examined under an electron microscope (JEM 1220: JEOL, Tokyo, Japan).

Suzuki H., Yamada K., Matsuda Y., Onozuka M, & Yamamoto T. (2017). CXCL14-like Immunoreactivity Exists in Somatostatin-containing Endocrine Cells, and in the Lamina Propria and Submucosal Somatostatinergic Nervous System of Mouse Alimentary Tract. Acta Histochemica et Cytochemica, 50(6), 149-158.

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Ultrastructural Analysis of Glioma Cells

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To investigate the cellular ultrastructure, glioma cells were washed three times with ice-cold phosphate-buffered saline (PBS; Sigma-Aldrich Corporation). The cells were collected after centrifugation (1,500 rpm for 5 min) and prefixed with 2.5% glutaraldehyde, then postfixed in 1% osmium tetroxide, dehydrated in ethanol gradients, impregnated with epoxy embedding resin (Fluka Epoxy Embedding Medium Kit; Sigma-Aldrich) and cut with an ultramicrotome (Leica EM UC6). Thin sections were poststained with uranyl acetate and lead citrate, and evaluated by a JEM-1220 (Jeol) TEM at 80 keV, with a Morada 11 megapixels’ camera (Olympus Soft Imaging Solutions).

Jaworski S., Biniecka P., Bugajska Ż., Daniluk K., Dyjak S., Strojny B., Kutwin M., Wierzbicki M., Grodzik M, & Chwalibog A. (2017). Analysis of the cytotoxicity of hierarchical nanoporous graphenic carbon against human glioblastoma grade IV cells. International Journal of Nanomedicine, 12, 3839-3849.

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Cardiac Tissue Preparation for TEM

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We fixed the hearts after the first ischemic phase with 2% paraformaldehyde and 2% glutaraldehyde at 4°C overnight. The tissue samples were cut to about three mm square followed by post-fixation with 1% osmium tetroxide in 0.1 M phosphate buffer. They were then dehydrated in a series of graduated ethanol solutions (50%, 60%, 70%, 80%, 90%, 99.6% and 100%, three times for each) at room temperature for 20 min each. Dehydrated samples were immersed into the half Epon (1:1 resin:ethanol mixture) overnight, followed by full Epon for an hour twice. We eventually obtained the solid pellet by embedding the samples into epoxy resin (Poly/Bed 812) at 60°C for 48 hr. An ultramicrotome equipped with a diamond knife set at 70 nm for the preparation of single section or serial sections was used to obtain ultra-thin sections. We collected these sections onto formvar-coated grids and stained them with EM stainer (Nisshin EM, Tokyo, Japan). We observed the cross-section samples using a transmission electron microscope (JEM-1220, JEOL, Tokyo, Japan) at the end of the first ischemic phase [34 (link)].

Ikemoto K., Hashimoto K., Harada Y., Kumamoto Y., Hayakawa M., Mochizuki K., Matsuo K., Yashiro K., Yaku H., Takamatsu T, & Tanaka H. (2021). Raman Spectroscopic Assessment of Myocardial Viability in Langendorff-Perfused Ischemic Rat Hearts. Acta Histochemica et Cytochemica, 54(2), 65-72.

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Multimodal Characterization of Magnetic Nanoparticles

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Base and Etop -MNPs were prepared for scanning transmission electron microscopy (S/TEM) and energy dispersive x-ray spectroscopy (EDS) analysis with 10 washes of OmniTrace® Ultra picopure water (EMD Millipore Corperation, Billerica, MA) to remove contaminants. MNPs were drop cast onto Lacey Carbon Grids (Electron Microscopy Sciences, Hatfield, PA) and dried sterilely for 24 h. MNPs were visualized using a JEOL-ARM200CF electron microscope (JEOL USA, Inc., Glen Ellyn, IL) operated at 200 keV. EDS line scan data was collected 0–10 keV with 10 eV per channel and dwell time of 15 μs.
Samples for scanning electron microscopy (SEM) were dehydrated with ethanol followed by hexamethyldisilazane. Glass coverslips were adhered to aluminum mounts, then sputter-coated with 6.0 nm of Pt/Pd in a low pressure argon atmosphere. Surface morphology was examined using a Hitachi S-3000N Variable Pressure SEM (Hitachi America, Ltd., Santa Clara, CA) using secondary and backscatter detectors. MNPs have a high electron density and therefore appear bright on the images. For transmission electron microscopy (TEM), cells were fixed in 2.5% glutaraldehyde, dehydrated by an ethanol series, and embedded in epoxy resin. Ultra-thin sections (70 nm) were collected and stained with uranyl acetate and lead citrate. Specimens were examined using a JEOL JEM-1220 transmission electron microscope at 80 kV.

Engelhard H.H., Willis A.J., Hussain S.I., Papavasiliou G., Banner D.J., Kwasnicki A., Lakka S.S., Hwang S., Shokuhfar T., Morris S.C, & Liu B. (2020). Etoposide-Bound Magnetic Nanoparticles Designed for Remote Targeting of Cancer Cells Disseminated Within Cerebrospinal Fluid Pathways. Frontiers in Neurology, 11, 596632.

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Electron Microscopy of EV Pellets

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EV pellets were fixed with 4% paraformaldehyde (PFA). Following a total of 8 washes using PBS, grids were contrasted with a uranyl-oxalate solution for 5 minutes and transferred to methyl-cellulose-uranyl acetate for 10 minutes on ice as previously described [16 (link)]. Samples were examined on a JEOL JEM-1220 transmission electron microscope (TEM) (JEOL USA, Inc.).

Xuan W., Wang L., Xu M., Weintraub N.L, & Ashraf M. (2019). miRNAs in Extracellular Vesicles from iPS-Derived Cardiac Progenitor Cells Effectively Reduce Fibrosis and Promote Angiogenesis in Infarcted Heart. Stem Cells International, 2019, 3726392.

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Ultrastructural Visualization of Cervical Root Dentin GAGs

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Spatial distribution and ultrastructure of GAGs in cervical root dentin were visualized in transmission electron microscope using previously described method [46 (link)]. Briefly, root dentin sections (n = 3) were fixed in formalin (1:10) for 4 days. Specimens were demineralized in 0.5 M EDTA (1.5 mL pH 8.0) at 4°C for 3 days and rinsed with ultrapure water. Specimens were then fixed in 2.5 vol% aqueous glutaraldehyde (1.5 mL, pH 5.8) overnight at 4°C, rinsed with 25 mM sodium acetate solution (pH 5.8), and stained with 0.05 vol/wt% cupromeronic blue solution for 3 h at 37 °C. Specimens were then rinsed with 25 mM NaOAc containing 0.1 M MgCl₂ and 2.5 vol% glutaraldehyde buffer for 30 min. Specimens were dehydrated in a series of solutions of ascending concentration of ethanol in water (25 to 90%) and then in absolute 100% ethanol and 100% propylene oxide. Specimens were infiltrated overnight in a 1:1 mixture of PO and LX-112 epoxy resin, and 2 h in 100% pure LX-112 resin. Sections of ~70 nm thickness (Leica Ultracut UCT model) were post-stained with 6% uranyl acetate and imaged (JEOL JEM-1220) at 80 kV.

Reis M., Alania Y., Leme-Kraus A., Free R., Joester D., Ma W., Irving T, & Bedran-Russo A.K. (2021). The Stoic Tooth Root: How the Mineral and Extracellular Matrix Counterbalance to Keep Aged Dentin Stable. Acta biomaterialia, 138, 351-360.

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Characterization of Silver Nanoparticles

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The hydrocolloid of nano-Ag (AgNPs) obtained from Nano-Tech (Warsaw, Poland) was produced by an electric non-explosive patented method (patent number US2009020364 A1) from high-purity metals (99.9999%) and high-purity demineralized water [7 ]. The physical and chemical properties of AgNPs were characterized by Chwalibog et al. [8 (link)]. The shape and size of NPs were inspected with a Jeol JEM-1220 transmission electron microscope (TEM) at 80 KeV (JEOL, Tokyo, Japan), with a Morada 11 megapixel camera (Olympus Soft Imaging Solutions GmbH, Münster, Germany) (Figure 1). Samples of Ag for TEM were prepared by placing droplets of hydrocolloids onto formvar-coated copper grids (Agar Scientific Ltd, Stansted, UK). Nanoparticles of Ag were mostly spherical and polydispersed. The stability of the colloidal dispersions of the nanoparticles (zeta potential) was measured by the electrophoretic light-scattering method with a Zetasizer Nano ZS, model ZEN3500 (Malvern Instruments, Worcestershire, UK). The zeta potential of Ag nanoparticles was −36.4 mV, and the average diameter of particles was 70 nm (Figure 1). AgNPs were dissolved in ultra-pure water (Milli-Q water system, Millipore Corp., Billerica, MA, USA).Figure 1

Size distribution and TEM image of silver nanoparticles.

Urbańska K., Pająk B., Orzechowski A., Sokołowska J., Grodzik M., Sawosz E., Szmidt M, & Sysa P. (2015). The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells. Nanoscale Research Letters, 10, 98.

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