Ammonium bicarbonate nh4hco3

Manufactured by Merck Group

Sourced in United States, Germany

Ammonium bicarbonate (NH4HCO3) is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline solid with a mildly alkaline taste. Ammonium bicarbonate is primarily used as a pH buffer, a leavening agent, and a source of carbon dioxide in various chemical and biological applications.

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Lab products found in correlation

Dithiothreitol (dtt), by Merck Group (7 mentions) Formic acid, by Merck Group (7 mentions) Urea, by Merck Group (5 mentions) Iodoacetamide, by Merck Group (5 mentions) Iodoacetamide iaa, by Merck Group (4 mentions) Sequencing grade modified trypsin, by Promega (4 mentions) Milli q water purification system, by Merck Group (4 mentions) Milli q system, by Merck Group (3 mentions) Trypsin, by Promega (3 mentions) Sequencing grade trypsin, by Promega (3 mentions) Acetonitrile, by Merck Group (3 mentions) Formic acid, by Thermo Fisher Scientific (3 mentions) Acetonitrile, by Thermo Fisher Scientific (3 mentions) Bovine pancreas tpck treated trypsin, by Merck Group (2 mentions) Trypsin sequencing grade, by Yuanye Bio-Technology (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Acetonitrile acn, by Merck Group (2 mentions) Dl dithiothreitol, by Merck Group (2 mentions) Acetic acid, by Thermo Fisher Scientific (2 mentions) Protease inhibitor mixture, by Merck Group (2 mentions)

37 protocols using ammonium bicarbonate nh4hco3

Mammalian Cell Lysis and Protein Extraction

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Bovine pancreas TPCK-treated trypsin, bovine serum albumin (BSA), urea, ammonium bicarbonate (NH₄HCO₃), dithiothreitol (DTT), iodoacetamide (IAA), octyl β-D-glucopyranoside, and angiotensin II (human, Asp-Arg-Val-Tyr-Ile-His-Pro-Phe) were purchased from Sigma–Aldrich (St. Louis, MO). Acetonitrile (ACN), acetic acid, formic acid (FA), and hydrofluoric acid (HF) were purchased from Fisher Scientific (Pittsburgh, PA). Methanol and water were purchased from Honeywell Burdick & Jackson (Wicklow, Ireland). Fused silica capillary (30 μm i.d./150 μm o.d.; 158 μm i.d./360 μm o.d.) and linear polyacrylamide (LPA) coated capillary (30 and 50 μm i.d./150 μm o.d.) were purchased from Polymicro Technologies (Phoenix, AZ).
Mammalian Cell-PE LB^™ buffer for cell lysis was purchased from G-Biosciences (St. Louis, MO). Complete, mini protease inhibitor cocktail (provided in EASYpacks) was purchased from Roche (Indianapolis, IN).

Sun L., Zhu G., Zhang Z., Mou S, & Dovichi N.J. (2015). A third-generation electro-kinetically pumped sheath flow nanospray interface with improved stability and sensitivity for automated capillary zone electrophoresis-mass spectrometry analysis of complex proteome digests. Journal of proteome research, 14(5), 2312-2321.

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Mass Spectrometry Protein Analysis

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The chemicals and reagents used in this work are the following: water (LC-MS grade-LiChrosolv^®) (Merck KGaA, Darmstadt, Germany), acetonitrile (LC-MS grade-LiChrosolv^®) (Merck KGaA, Darmstadt, Germany), 2-propanol (LC-MS grade-LiChrosolv^®) (Merck KGaA, Darmstadt, Germany), ammonium bicarbonate (NH₄HCO₃) (Sigma-Aldrich, Buchs, Switzerland), RapiGest^TM SF surfactant (Waters, Milford (MA), USA), DL-dithiothreitol (DTT) (Sigma-Aldrich, Buchs, Switzerland), iodoacetamide (IAA) (Sigma-Aldrich, Buchs, Switzerland), trypsin (porcine pancreas) (Sigma-Aldrich, Buchs, Switzerland), trifluoroacetic acid (TFA) (Honeywell SC, Seeelze, Germany), formic acid (FA) LiChropur^® (Merck KGaA, Darmstadt, Germany), Pierce^TM HeLa protein digest standard (Thermo Scientific^TM, Waltham, MA, USA) and MMI-L low concentration tuning mix (Agilent Technologies, Santa Clara, CA, USA).

Previtali P., Pagani L., Risca G., Capitoli G., Bossi E., Oliveira G., Piga I., Radice A., Trezzi B., Sinico R.A., Magni F, & Chinello C. (2023). Towards the Definition of the Molecular Hallmarks of Idiopathic Membranous Nephropathy in Serum Proteome: A DIA-PASEF Approach. International Journal of Molecular Sciences, 24(14), 11756.

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Chitosan-based Cryogels for Antibiotics

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Natural Kaolin (K, Acros Organics) and the silane coupling agent, γ-methacryloxypropyltrimethoxysilane (MAPTES, by Sigma Aldrich, 98% purity), were used without further purification. In order to obtain the three cryogels series, the following types of chitosan were used: (i) commercial chitosan (CC, ≥75% deacetylation degree, Mn = 2.056 × 10⁵ g/mol, supplied by Sigma Aldrich and used as received); (ii) chitosan from commercial chitin (CCH, 77% deacetylation degree, Mn = 4.7291 × 10⁵ g/mol) prepared according to Miron et al. [53 (link)]; and (iii) chitosan from shrimp shells (CSH, 76% deacetylation degree, Mn = 9.058 × 10³ g/mol), prepared according to Miron et al. [53 (link)]. Pure demineralized water (Millipore) was used to solubilize the chitosan. Biocellulose (BC) was kindly provided by our collaborator from the University “Politehnica” of Bucharest in the form of a swollen membrane as described by Frone et al. [54 (link)]. Penicillin G (PG, purity ≥ 100%, M = 356 g/mol), acetic acid (99%), and ammonium bicarbonate (NH₄HCO₃, 99.5%) were purchased from Sigma-Aldrich.

Dumitru M.V., Sandu T., Miron A., Zaharia A., Radu I.C., Gavrilă A.M., Sârbu A., Iovu H., Chiriac A.L, & Iordache T.V. (2023). Hybrid Cryogels with Superabsorbent Properties as Promising Materials for Penicillin G Retention. Gels, 9(6), 443.

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Proteomic Sample Preparation Protocol

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MgT was purchased from Shanghai Yuanye Biotechnology Co., CAS number is 778571–57-6, molecular formula is C8H14MgO10, purity is more than 95%. Trypsin Golden (Promega, United States), dithiothreitol (DTT, Sigma, Germany), iodoacetamide (IAA, Sigma, Germany), ammonium bicarbonate NH4HCO3 (Sigma, Germany), urea (Sigma, Germany), pure water (China Wahaha Company), mass spectrometry grade methanol (Thermo Fisher Scientific, United States), mass spectrometry grade acetonitrile (Thermo Fisher Scientific, United States), mass spectrometry grade purified water (Thermo Fisher Scientific, United States), Tris-base (Promega, United States), thiosulfate (Sigma, Germany), thiamine (Sigma, Germany), thiophene (Promega, United States), and thiourea (Sigma, Germany) were obtained.

Shen Z., Yang M., Wang H., Liu Y, & Gao Y. (2023). Changes in the urinary proteome of rats after short-term intake of magnesium L-threonate(MgT). Frontiers in Nutrition, 10, 1305738.

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Trypsin-based In-Gel Protein Digestion

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Dried HPLC fractions were dissolved in loading buffer (LB1X: 2% SDS BIORAD, 50mM TRIS-HClpH6.8, 10% Glycerol SIGMA, and bromophenol blue BIORAD), fractionated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and stained with GelCode™ Blue Safe Protein Stain (Thermo Fisher Scientific). After destaining, two bands were cut from lanes 22 and 26, respectively. The bands were in situ hydrolyzed by trypsin as reported in [39 (link)]. Briefly, gel bands were further destained alternating washes with acetonitrile (ACN) (Honeywell, Charlotte, NC, USA), 50 mM ammonium bicarbonate (NH4HCO3) (Sigma, St. Louis, MO, USA), and cysteine residues reduced by 10 mM of dithiothreitol (Sigma, St. Louis, MO, USA), and then alkylated in 55 mM iodoacetamide (Sigma, St. Louis, MO, USA). Following extensive washings to remove the excess reagents, gel bands were then treated with trypsin. Peptide mixtures were extracted in 0.2% HCOOH and ACN and vacuum dried by a Savant SpeedVac System (Thermo Fisher Scientific, Waltham, MA, USA).

Salvia R., Cozzolino F., Scieuzo C., Grimaldi A., Franco A., Vinson S.B., Monti M, & Falabella P. (2022). Identification and Functional Characterization of Toxoneuron nigriceps Ovarian Proteins Involved in the Early Suppression of Host Immune Response. Insects, 13(2), 144.

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Xenopus laevis Embryo Proteomics

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Bovine pancreas TPCK-treated trypsin, urea, ammonium bicarbonate (NH₄HCO₃), dithiothreitol (DTT), and iodoacetamide (IAA) were purchased from Sigma–Aldrich (St. Louis, MO). Acetonitrile (ACN) and formic acid (FA) were purchased from Fisher Scientific (Pittsburgh, PA). Water was deionized by a Nano Pure system from Thermo scientific (Marietta, OH). iTRAQ 8-plex kits were purchased from AB Sciex (Foster City, CA).
Xenopuslaevis was purchased from Nasco (Fort Atkinson, WI). Mammalian Cell-PE LB™ buffer for embryo lysis was purchased from G-Biosciences (St. Louis, MO). Complete, mini protease inhibitor cocktail (provided in EASYpacks) was purchased from Roche (Indianapolis, IN).

Sun L., Bertke M.M., Champion M.M., Zhu G., Huber P.W, & Dovichi N.J. (2014). Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development. Scientific Reports, 4, 4365.

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Synthesis of Nickel Carbonate Hydroxide

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Nickel carbonate hydroxide was synthesized by a hydrothermal method (Scheme 1).^{17 (link)} Typically, 2.5 mmol nickel nitrate hydrate (Ni(NO₃)₂·6H₂O) (99%, Sigma-Aldrich) was dissolved in 200 mL deionized (DI) water, while 5 mmol ammonium bicarbonate NH₄HCO₃ (99%, Sigma-Aldrich) in 50 ml DI water. The two solutions were mixed and stirred for 10 minutes. To achieve a pH ≈ 6.5, citric acid (0.1 g ml⁻¹ in DI water) was added to the solution while stirring. The resulting solution was transferred to a Teflon-lined stainless-steel autoclave and hydrothermally treated at 120 °C overnight. After the autoclave was naturally cooled down to room temperature, the sample was taken out, washed with DI water several times, and dried under vacuum overnight. The obtained product was labeled as NCH. The possible chemical reactions involved in the aforementioned preparation process are depicted below.

Talebi P., Greco R., Yamamoto T., Zeynali M., Asgharizadeh S, & Cao W. (2024). Hierarchical nickel carbonate hydroxide nanostructures for photocatalytic hydrogen evolution from water splitting. Materials Advances, 5(7), 2968-2973.

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Hydrophilic and Hydrophobic TPU Characterization

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The hydrophilic TPU Pathway^® PT83AE100 (HPL, shore hardness = 83 A, nominal water uptake = 100 wt%) and the hydrophobic TPU Pathway^® PT72AE (HPB, shore hardness = 72 A) were kindly provided by Lubrizol LifeSciences, Wickliffe, OH, USA. Ammonium bicarbonate (NH₄HCO₃) and sodium bicarbonate (NaHCO₃), supplied by Sigma Aldrich, Vienna, Austria, were used as pore formers (PFs). Sodium hydroxide, potassium dihydrogen phosphate (all by Sigma Aldrich, Vienna, Austria) and water purified by TKA MicroPure UV (JWT GmbH, Jena, Germany) were used to prepare the aqueous media.

Koutsamanis I., Spoerk M., Arbeiter F., Eder S, & Roblegg E. (2020). Development of Porous Polyurethane Implants Manufactured via Hot-Melt Extrusion. Polymers, 12(12), 2950.

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Glycoproteomic Sample Preparation Protocol

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HPLC-grade acetonitrile (ACN), HPLC-grade water, HPLC-grade methanol, hydrochloric acid (HCl), and sodium chloride (NaCl) were obtained from DUKSAN (Gyungkido, Korea). The BCA protein assay kit was purchased from Pierce (Hercules, CA), and Complete Protease Inhibitor Cocktail Mini Tablets were purchased from Roche (Mannheim, Germany). Dithiothreitol (DTT) and urea were purchased from AMRESCO (Solon, OH). PMSF, Sodium dodecyl sulfate (SDS) and Tris were purchased from USB (Cleveland, OH). Sequencing-grade modified trypsin and LysC were purchased from Promega Corporation (Madison, WI) and Wako (Osaka, Japan), respectively.
All other reagents—concanavalin A (ConA), wheat germ agglutinin (WGA), Ricinus communis agglutinin 120 (RCA120), 2-mercaptoethanol, ammonium bicarbonate (NH₄HCO₃), sucrose, EDTA, formic acid, iodoacetamide (IAA), trifluoroacetic acid (TFA), PNGase F, and stable isotope-labeled water (H₂ ¹⁸O, 99% atom% ¹⁸O)—were purchased from Sigma-Aldrich (St. Louis, MO).

Han D., Moon S., Kim Y., Min H, & Kim Y. (2014). Characterization of the membrane proteome and N-glycoproteome in BV-2 mouse microglia by liquid chromatography-tandem mass spectrometry. BMC Genomics, 15, 95.

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Protein Sample Preparation Protocol

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Deionized (DI) water (18.2 MΩ·cm) was produced by a Milli-Q system (Millipore, Bedford, MA, United States) and used in all experiments. HPLC grade acetonitrile (ACN) and methanol as well as analytical grade acetone and hydrochloric acid (HCl, 37%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Analytical grade formic acid was purchased from J&K Scientific Ltd. (Beijing, China). Iodoacetamide (IAA), trizma base, urea, sodium dodecyl sulfate (SDS), and ammonium bicarbonate (NH₄HCO₃) were purchased from Sigma-Aldrich (St. Louis, MI, United States). Bond-breaker TCEP solution (0.5 mol/L) and protease inhibitor cocktail (100×, EDTA-free) were purchased from Thermo Fisher Scientific (Rockford, United States). Trypsin used for protein digestion was bought from Hualishi Technology Co., Ltd. (Beijing, China). Trypsin–EDTA solution (0.25%, with phenol red) used for cell digestion and phosphate buffered saline (PBS, 1×) were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China).

Lu J., Liu W., Chen X.Z., Wang Y., Ying T., Qiao L., Liu Y.J, & Liu B. (2023). Temporal proteomic profiling reveals functional pathways in vaccinia virus-induced cell migration. Frontiers in Microbiology, 14, 1185960.

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