For the GC-MS analysis, 1000 µg/mL of CN-Dcm was dissolved in 1 mL of HPLC grade methanol. The secondary metabolites from CN-Dcm were determined using an Agilent gas chromatograph model 6890 equipped with an Agilent 19091S-433 capillary column, (5%-phenyl)-methylpolysiloxane phase (HP-5MS) 0.25 mm × 30 m × 0.25µm) (Santa Clara, CA, USA). Helium gas was used as the carrier gas at 1.0 mL per minute with split mode injection. The oven temperature was set as follows: 70 °C was held for 2 min, then it was increased to 280 °C at a rate of 20 °C per min for 20 min. The total run time was 32.50 min.
Gas chromatograph model 6890
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 6890 Gas Chromatograph is a versatile analytical instrument designed for the separation and identification of volatile and semi-volatile compounds. It features a temperature-controlled oven, a variety of injector options, and multiple detector choices to accommodate diverse analytical needs. The 6890 Gas Chromatograph provides reliable performance and precise data for a wide range of applications.
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Lab products found in correlation
8 protocols using gas chromatograph model 6890
1
GC-MS Analysis of Secondary Metabolites
2
GC-MS Analysis of Volatile Compounds
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GC-MS analyses of volatile compounds from the three crude ethanolic leaf extracts of KL, MO and NE were performed using a Hewlett Packard Gas Chromatograph model 6890 (Agilent Technologies, Palo Alto, CA, USA) equipped with a mass selective detector. The chemical components in all extracts were separated in a silica capillary Hewlett Packard HP-5 (5% phenyl methyl siloxane) column (30 m × 0.25 mm i.d., 0.25 µm film thickness). Pure helium was used as the carrier gas with a constant flow rate at 13.7 mL/min. Initial temperature was set at 250 °C with split ratio mode at ratio 10:1 and 1 µL injector volume. The oven temperature was started at 70 °C for 3 min with increasing rate of 5 °C/min to 280 °C and holding time of about 20 min. Transfer temperature was set at 280 °C and mass detection ranges were set from 50 to 700 amu in full scan.
Identification of volatile compounds was performed by computer matching of their recorded mass spectra fragmentation patterns in the Wiley 7n MS spectral library or with previously published data. Identified compounds from the three plants were expressed as percentages based on the peak areas produced in the chromatogram.
Identification of volatile compounds was performed by computer matching of their recorded mass spectra fragmentation patterns in the Wiley 7n MS spectral library or with previously published data. Identified compounds from the three plants were expressed as percentages based on the peak areas produced in the chromatogram.
3
GC-MS Analysis of Plant Extract
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The 1 mg/mL of selected extract was dissolved with high-performance liquid chromatography (HPLC)grade methanol (Merck, Malaysia). The compound extract was identi ed using an Agilent gas chromatograph model 6890 equipped with an Agilent 19091S-433 capillary column, HP-5MS (0.25 mm x 30 m x 0.25 µm) (California, United States). The carrier gas, helium was used at 1.0 mL per minute. The oven was set to the following temperature: 70 °C was retained for 2 min, then it was elevated to 280 °C at the rate of 20 °C per min for 20 min. The GC-MS analysis was run for 32.5 min. The compounds of selected extract were identi ed by comparing the similarity index of at least 80% with the compounds from National Institute of Standards and Technology (NIST) library.
4
GC-MS Analysis of Plant Extract
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The 1 mg/mL of selected extract was dissolved with high-performance liquid chromatography (HPLC)grade methanol (Merck, Malaysia). The compound extract was identi ed using an Agilent gas chromatograph model 6890 equipped with an Agilent 19091S-433 capillary column, HP-5MS (0.25 mm x 30 m x 0.25 µm) (California, United States). The carrier gas, helium was used at 1.0 mL per minute. The oven was set to the following temperature: 70 °C was retained for 2 min, then it was elevated to 280 °C at the rate of 20 °C per min for 20 min. The GC-MS analysis was run for 32.5 min. The compounds of selected extract were identi ed by comparing the similarity index of at least 80% with the compounds from National Institute of Standards and Technology (NIST) library.
5
Compound identification in bioactive fraction
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The fraction F3 that is consistently exhibiting activity in the assays was analysed on GC-MS to determine the various compounds present in it with their appropriate masses. It was dissolved in n-hexane using an Agilent gas chromatograph Model 6890, coupled to a Mass spectrometer equipped with a DB DB-1MS capillary column (30m long × 320 μm nominal diameter), programmed and ran to obtain the mass spectra data. The compounds from fraction F3 were identified by comparing their retention time and mass with those of reference compounds in the NIST library. Acceptance was based on a quality factor greater than 80.
6
GC-MS Analysis of Lipid Samples
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Lipid samples were silylated for GC analysis [68 (link)], and a 1-µL aliquot was injected into an Agilent Technologies Model 6890 Gas Chromatograph equipped with an Agilent 19091S-433 column (30.0 m × 250 µm × 0.25 µm). MS-analysis was conducted by coupling the column eluent to a Model 5973 Mass Selective Detector capable of electrical ionization (EI). The chromatography temperature program initially started at 50 °C for 1 min, then raised at a rate of 25 °C per min to 200 °C, held at 200 °C for 2 min, and again raised at a rate of 10 °C per min to 280 °C and maintained at that temperature for 2 min, then raised at 20 °C per min to 320 °C, where it was maintained for 20 min.
7
Amino Acid Analysis of Nectar
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Analysis of amino acids was performed using the Phenomenex EZ:FaastTM kit for free amino acids (Torrance, CA, United States). Each sample (60 μL nectar per extraction) consisted of nectar pooled from four individual flowers. Six replications were analyzed for each sex. Sample preparation from solid phase extraction to derivatization were completed according to the manufacturer with one adjustment: after addition of the norvaline internal standard to each sample, 125 μL of 10% propanol/20 mM HCl was added to acidify the sample. Following derivatization, samples were concentrated under a stream of nitrogen gas before amino acids were analyzed using an Agilent Technologies model 6890 gas chromatograph coupled to a model 5973 mass selective detector capable of electrical ionization (EI). The GC-MS instrument settings followed the manufacturer’s recommendations.
8
In-House Ion Mobility Spectrometer
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An ion mobility spectrometer was built in-house (Figure S3 ) from a general structure and materials already
described in detail and with a few modifications.31 (link) The ion source was a corona discharge and data acquisition
was made using an 18-bit PCI-6281 DAQ interface card (National Instruments
Corp., Austin, TX, USA) and a LabView program developed in-house and
labeled Linear 2018 version 2.0. Data were acquired at 166.7 kHz for
28 ms spectra with a temporal resolution of 6 μs and a spectral
averaging of 20 ms. The pulse width used for opening and closing the
ion shutter was set to ∼120 μs. Note that this instrument
has two ion shutters with the second one continuously kept open (i.e.,
inactive) during the measurements. The drift tube was combined with
a Model 6890 gas chromatograph (Agilent Corp., Avondale, PA, USA)
equipped with a 15 m RTX-5 capillary column with a nitrogen carrier
gas. The drift gas (air) was purified using activated carbon and molecular
sieves with a flow of 380 mL.min–1 and at source
vent, the drift gas temperature was raised to 60 °C by heating
the flange to a temperature of 120 °C.
described in detail and with a few modifications.31 (link) The ion source was a corona discharge and data acquisition
was made using an 18-bit PCI-6281 DAQ interface card (National Instruments
Corp., Austin, TX, USA) and a LabView program developed in-house and
labeled Linear 2018 version 2.0. Data were acquired at 166.7 kHz for
28 ms spectra with a temporal resolution of 6 μs and a spectral
averaging of 20 ms. The pulse width used for opening and closing the
ion shutter was set to ∼120 μs. Note that this instrument
has two ion shutters with the second one continuously kept open (i.e.,
inactive) during the measurements. The drift tube was combined with
a Model 6890 gas chromatograph (Agilent Corp., Avondale, PA, USA)
equipped with a 15 m RTX-5 capillary column with a nitrogen carrier
gas. The drift gas (air) was purified using activated carbon and molecular
sieves with a flow of 380 mL.min–1 and at source
vent, the drift gas temperature was raised to 60 °C by heating
the flange to a temperature of 120 °C.
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