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Alexa 568 conjugated anti rabbit igg

Manufactured by Abcam
Sourced in United Kingdom

Alexa Fluor 568-conjugated anti-rabbit IgG is a secondary antibody conjugated to the Alexa Fluor 568 fluorescent dye. It is designed to detect and visualize rabbit primary antibodies in immunological applications such as immunofluorescence, Western blotting, and flow cytometry.

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2 protocols using alexa 568 conjugated anti rabbit igg

1

Parvalbumin Immunofluorescence in Mouse Brain

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After physiological experiments, mice were deeply anesthetized with isoflurane or urethane and transcardially perfused with 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS). The brain was removed and post-fixed in 4% PFA at 4 °C for one day. Coronal sections of 50–100 µm thickness were obtained using a microslicer (DTK-1000N, Dosaka EM). For immunofluorescence, the sections were incubated at room temperature in PBS (5 min × 3) and in PBS containing 0.3% Triton X-100 (PBST) for 5 min. The sections were further incubated in the blocking solution (5% normal goat serum, 1% bovine serum albumin, and 0.3% Triton X-100 in PBS) for 60 min at room temperature. We then incubated the sections with a rabbit polyclonal antibody to parvalbumin (1:2000, Abcam) in the blocking solution at 4 °C overnight, washed with PBST (5 min × 3), and incubated with Alexa 568-conjugated anti-rabbit IgG (1:500, Abcam) for 2 h at room temperature. The sections were mounted on glass slides, and the fluorescence images were taken with an inverted fluorescence microscope (BZ-X800, Keyence).
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2

Immunostaining of GD3 and GD2 in Mice

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Mouse anti-GD3 monoclonal antibody (mAb) (R24) was obtained from Dr. L. J. Old (Memorial Sloan-Kettering Cancer Center, New York, USA). Anti-GD2 mAb 220-51 was as described previously.31 (link) Rat anti-mouse CD68 (MCA1957) and rabbit anti-mouse iNOS (PA1-036) were purchased from AbDSerotec (Kidlington, UK). Rabbit anti-Iba1 antibody was from Wako (Cat. No. 019-19741, Osaka, Japan). Alexa568-conjugated anti-rabbit IgG and Alexa488-conjugated anti-rat IgG were purchased from Abcam (Cambridge, UK). Alexa546-conjugated anti-mouse IgG and DAPI were from Thermo Fisher Scientific (Waltham, MA, USA).
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