The diacetylenic lipids, PCDA, TCDA and HCDA (8,10-heneicosadiynoic acid) were purchased from GFS Chemicals, OH, USA. Film thicknesses were measured using an alpha step instrument. Contact angle data were obtained using a PHX300, Korea. Raman spectra were obtained using laser excitation at 785 nm and a Raman microscope (Kaiser Optical Systems). SEM images were obtained with a JEOL(JSM-6330F) FE-SEM, and optical/fluorescence microscopic images were collected with a Olympus BX 51W/DP70. XRD spectra were recorded with a HR-XRD, D8 discover, Bruker. Friction ridge structures on a fingertip were obtained using a video microscope system (Camscope SV 32) with a prism. A homemade humidity-controlled chamber was used for hydrochromism tests under various humidity conditions.
Jsm 6330f fe sem
Manufactured by JEOL
The JSM-6330F FE-SEM is a field emission scanning electron microscope (FE-SEM) manufactured by JEOL. It is designed to provide high-resolution imaging and analysis of a wide range of materials. The FE-SEM utilizes a field emission electron source to generate a focused electron beam that scans the surface of a sample, generating various signals that are detected and used to create an image.
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2 protocols using jsm 6330f fe sem
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Diacetylenic Lipid Characterization
2
Multimodal Imaging of Leaf Stomata
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Examination of the optical and fluorescence images of the leaves was conducted using an Olympus (BX51 W/DP70) microscope with a U-MWG2 filter set (510–550 nm excitation, exposure time of 142 ms). UV-visible absorption spectra were recorded using a single beam Agilent 8453 UV–visible Spectrometer (Agilent Technologies). The Raman spectra of the PDA film at locations on the leaf for active and inactive stomata respectively were collected with a Raman microscope (Horiba Scientific, LabRAM HR Evolution) using a 785 nm excitation laser. The leaf sample was immobilized onto a glass slide using double sided tape. For scanning electron microscopy (SEM), the leaf samples were immobilized onto an SEM plate using carbon tape as well as silver paste, and the leaves were sputter coated with platinum prior to SEM. SEM of the stomata was conducted with a JEOL(JSM-6330F) FE-SEM.
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