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E coli ec100dpir

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The E. coli EC100Dpir+ is a competent bacterial strain used for the transformation and amplification of DNA plasmids. It is a commonly used host cell line in molecular biology and genetic engineering applications.

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Lab products found in correlation

Gel extraction kit, by Thermo Fisher Scientific (2 mentions) Phusion high fidelity dna polymerase, by New England Biolabs (2 mentions) Genejet pcr purification, by Thermo Fisher Scientific (2 mentions) Pjet1, by Thermo Fisher Scientific (2 mentions) Restriction enzyme, by New England Biolabs (2 mentions)

2 protocols using e coli ec100dpir

1

Molecular Cloning Techniques for DNA Manipulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Conventional plasmid isolation and DNA manipulation techniques were used as described in Sambrook and Russell (44 ). Sanger sequencing was sourced to Elim Biopharm (California) and reaction composition were as per their instruction. Restriction enzymes and high-fidelity Phusion DNA polymerase were obtained from New England Biolabs (Ipswich, MA). Primers used in this study are listed in Supplemental Table 10. For subcloning of PCR products pJET1.2 was used as an intermediate PCR cloning vector (ThermoScientific, Waltham, MA). GeneJET PCR purification, plasmid miniprep, and gel extraction kits (ThermoScientific, Waltham, MA) were routinely used. Ligation products were transformed by electroporation into E. coli EC100Dpir+ (Epicentre, Madison, WI).
Loftie-Eaton W., Bashford K., Quinn H., Dong K., Millstein J., Hunter S., Thomason M.K., Merrikh H., Ponciano J.M, & Top E.M. (2017). Compensatory mutations improve general permissiveness to antibiotic resistance plasmids. Nature ecology & evolution, 1(9), 1354-1363.
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2

Molecular Cloning Techniques for DNA Manipulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Conventional plasmid isolation and DNA manipulation techniques were used as described in Sambrook and Russell (44 ). Sanger sequencing was sourced to Elim Biopharm (California) and reaction composition were as per their instruction. Restriction enzymes and high-fidelity Phusion DNA polymerase were obtained from New England Biolabs (Ipswich, MA). Primers used in this study are listed in Supplemental Table 10. For subcloning of PCR products pJET1.2 was used as an intermediate PCR cloning vector (ThermoScientific, Waltham, MA). GeneJET PCR purification, plasmid miniprep, and gel extraction kits (ThermoScientific, Waltham, MA) were routinely used. Ligation products were transformed by electroporation into E. coli EC100Dpir+ (Epicentre, Madison, WI).
Loftie-Eaton W., Bashford K., Quinn H., Dong K., Millstein J., Hunter S., Thomason M.K., Merrikh H., Ponciano J.M, & Top E.M. (2017). Compensatory mutations improve general permissiveness to antibiotic resistance plasmids. Nature ecology & evolution, 1(9), 1354-1363.
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+ Expand

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