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2 protocols using 20 s rg3

1

Ginsenoside Standards Evaluation in Cell Viability

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Dulbecco modified Eagle medium (DMEM) and fetal bovine serum (FBS) were purchased from Invitrogen Co. (Grand Island, NY, USA). EZ-Cytox enhanced cell viability assay kit was purchased from ITSBIO (Seoul, Korea). Ginsenoside standards Rb1, Rb2, Rc, Rd, Re, 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5 were purchased from Ambo Institute (Seoul, Korea). Monoclonal antibodies against cleaved caspase-8 and β-actin and polyclonal antibodies against cleaved caspase-3, cleaved caspase-9, Bcl-2, Bax, and poly (ADP-ribose) polymerase (PARP) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The water and acetonitrile used were of HPLC grade from Fisher Scientific (Pittsburgh, PA, USA) and glacial acetic acid was of analytical grade from Sigma-Aldrich (Saint Louis, MO, USA).
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2

Ginsenoside and Amino Acid Analysis

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Ginsenoside standards Rb1, Rb2, Rc, Rd, Re, 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5 were purchased from Ambo Institute (Seoul, Korea). L-Amino acid standards alanine, arginine, glutamine, glycine, leucine, lysine, serine, trans-4-hydroxy-L-proline, and valine were purchased from Sigma Aldrich (Saint Louis, MO, USA). Monoclonal antibodies against cleaved caspase-8 and β-actin and polyclonal antibodies against cleaved caspase-3, cleaved caspase-9, BH3-interacting domain death (BID) agonist, and poly (ADP-ribose) polymerase (PARP) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).
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