Tecnai g2 spirit biotwin tem

Manufactured by Olympus

The Tecnai G2 Spirit BioTWIN TEM is a transmission electron microscope (TEM) designed for biological sample imaging and analysis. It provides high-resolution imaging capabilities for studying cellular structures, macromolecular complexes, and other nanoscale biological features.

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Lab products found in correlation

Sis veleta ccd camera, by Olympus (2 mentions) Tia software, by Thermo Fisher Scientific (1 mentions) Veleta ccd camera, by Olympus (1 mentions) Diamond knife, by Electron Microscopy Sciences (1 mentions) Uc7 ultramicrotome, by Leica (1 mentions)

Close spelling variants of this product

Tecnai G2 Spirit (6 citations) Tecnai G2 Spirit BioTWIN (5 citations) Tecnai Biotwin TEM (3 citations) Tecnai G2 TEM (3 citations) Spirit G2 (2 citations)

3 protocols using tecnai g2 spirit biotwin tem

Afferent Fiber Stimulation and Presynaptic Recordings

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Detailed methods are in Supplemental Experimental Procedures.
Afferent fiber stimulation and presynaptic recordings were performed as previously described on P16-21 mice (Chen et al., 2013 ; Chen et al., 2015 (link)). Confocal images were acquired with a Zeiss LSM 780 and analysed using Amira 5.6 and Fiji imaging analysis software. For EM experiments, Tecnai G2 Spirit BioTwin TEM was used and images were taken with a Veleta CCD camera (Olympus) operated by TIA software (FEI) and analyzed using Fiji. All procedures were performed in accordance with the animal welfare guidelines of MPFI Institutional Animal Care and Use Committee.

Montesinos M.S., Dong W., Goff K., Das B., Guerrero-Given D., Schmalzigaug R., Premont R.T., Satterfield R., Kamasawa N, & Young SM J.r. (2015). Presynaptic deletion of GIT proteins results in increased synaptic strength at a mammalian central synapse. Neuron, 88(5), 918-925.

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Electron Microscopy of Extracellular Vesicles

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Purified EVs were absorbed onto formvar/carbon-coated glow discharged copper EM grids (5 μL on each grid) for 20 min and fixed with 2% formaldehyde (Hatfield, MA, USA) for 20 min. After washing in 10 drops of distilled water, the grids were stained with 2% Uranyl acetate (Hatfield, MA, USA) for 5 min. Transmission electron microscopy was performed using a FEI Tecnai G2 Spirit BioTWIN TEM operating at an accelerating voltage of 120 keV. Images were acquired using an Olympus-SIS Veleta CCD Camera.

Carvalho A.S., Baeta H., Henriques A.F., Ejtehadifar M., Tranfield E.M., Sousa A.L., Farinho A., Silva B.C., Cabeçadas J., Gameiro P., da Silva M.G., Beck H.C, & Matthiesen R. (2021). Proteomic Landscape of Extracellular Vesicles for Diffuse Large B-Cell Lymphoma Subtyping. International Journal of Molecular Sciences, 22(20), 11004.

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Ultrastructural Analysis of Skin Cells

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All reagents and materials were purchased from Electron Microscopy Sciences unless otherwise stated. Specimens were fixed in 2% paraformaldehyde, 2% glutaraldehyde in 0.1 M phosphate buffer (PB) at pH 7.4 overnight at 4°C. After washing with PB, specimens were post‐fixed for 1 h on ice using 1% osmium tetroxide and 1.5% potassium ferrocyanide in distilled water. Specimens were subsequently dehydrated with a series of increasing ethanol concentrations (35%, 45%, 50%, 70%, 90%, 2× 100%) before infiltrating and embedding in Epon resin. After polymerising at 70°C overnight, resin blocks were sectioned at 90–100 nm using a UC7 ultramicrotome (Leica) and a diamond knife (Diatome), and sections collected on formvar/carbon‐coated copper mesh grids. Sections on grids were post‐stained with lead citrate and imaged using a Hitachi H‐7650 TEM equipped with an AMT XR41 M digital camera, or an FEI Tecnai G2 Spirit BioTWIN TEM equipped with an Olympus‐SIS Veleta CCD camera.
One specimen of RHPS and three specimens of RHPE were processed and observed by TEM. Quantitative TEM analysis was performed on a single specimen of RHPE, which was representative of the three biological replicates. Melanocytes and keratinocytes were identified and distinguished using criteria listed in Table 1.

Hall M.J., Lopes‐Ventura S., Neto M.V., Charneca J., Zoio P., Seabra M.C., Oliva A, & Barral D.C. (2022). Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer. Pigment Cell & Melanoma Research, 35(4), 425-435.

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