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70s6 kinase

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70S6 kinase is an enzyme that phosphorylates the 40S ribosomal protein S6, which is involved in the regulation of protein synthesis. It plays a role in cell growth and proliferation.

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Lab products found in correlation

Phospho ulk1 ser555, by Cell Signaling Technology (1 mentions) Rose bengal, by Merck Group (1 mentions) Phospho ampk thr172, by Cell Signaling Technology (1 mentions) Phospho mtor ser2448, by Cell Signaling Technology (1 mentions) Phospho 4e bp1 thr37 46, by Cell Signaling Technology (1 mentions) Phospho jnk thr183 tyr185, by Cell Signaling Technology (1 mentions) Phospho erk thr202 tyr204, by Cell Signaling Technology (1 mentions) Phospho ulk1, by Cell Signaling Technology (1 mentions) Mouse monoclonal antibody to ha 901503, by BioLegend (1 mentions) Mouse anti flag f3165, by Merck Group (1 mentions) Phospho p70 s6 kinase thr389 9234, by Cell Signaling Technology (1 mentions) Acp 196, by Selleck Chemicals (1 mentions) Phospho p70 s6 kinase, by Cell Signaling Technology (1 mentions) Poly adp ribose polymerase, by Cell Signaling Technology (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) β actin, by Abcam (1 mentions) Mk 2206, by Selleck Chemicals (1 mentions) Gapdh, by Proteintech (1 mentions) Annexin 5 fitc apoptosis kit, by BestBio (1 mentions)

3 protocols using 70s6 kinase

1

Regulation of MAPK and Autophagy Signaling

Cited 1 time
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Experiments were performed as previously described [22 (link)]. Antibodies specific for ERK (#4695), JNK (#9252), p38 (#9212), phospho-ERK (Thr202/Tyr204) (#4370), phospho-JNK (Thr183/Tyr185) (#4668), phospho-p38 (Thr180/Tyr182) (#9211), LC3B (#2775S), mTOR (#2983), phospho-mTOR (Ser2448) (#2971), ULK1 (#8054), phospho-ULK1 (Ser555) (#5869), phospho-ULK1 (Ser757) (#14202), phospho-70S6 kinase (Thr389) (#9234), 70S6 kinase(#2708), phospho-4E-BP1 (Thr37/46) (#2855), 4E-BP1 (#9452), AMPK (#2603) and phospho-AMPK (Thr172) (#2531) were purchased from Cell Signaling Technology (Danvers, MA). Antibodies against DUSP5 (#ab200708) were from Abcam (Cambridge, MA) and antibodies against p62 (#PM045) were from MBL Life science. Specific inhibitors U0126 (#HY-12031A), SB203580 (#HY-10256), SP600125 (#HY-12041) and bafilomycin A1 (#HY-100558) were obtained from MCE. Rapamycin (#V900930-1MG) and rose bengal (#330000) were from Sigma. U0216 (10 μM, 24 h), SB203580 (20 μM, 24 h), SP600125 (15 μM, 24 h), bafilomycin A1 (25 nM, 6 h) and rapamycin (100 nM, 12 h) were added to the cell cultures as described in different experiments. The primers for DUSP5 were TGTCGTCCTCACCTCGCTA and GGGCTCTCTCACTCTCAATCTTC.
Hua C., Zheng Q., Zhu J., Chen S., Song Y., van der Veen S, & Cheng H. (2022). Human Papillomavirus Type 16 Early Protein E7 Activates Autophagy through Inhibition of Dual-Specificity Phosphatase 5. Oxidative Medicine and Cellular Longevity, 2022, 1863098.
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2

Western Blotting Antibody Dilutions

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All antibodies were used at a dilution of 1:1,000–3,000 in TBST buffer with 5% non-fat milk for Western blotting. Antibodies to mTOR (2932), GβL (3274), Sin1 (12860), phospho-AKT Ser473 (4060), phospho-AKT Thr308 (13038), AKT (pan; 4691), Rictor (2114), phospho-p70 S6 kinase Thr389 (9234), p70 S6 kinase (9202), PDK1 (13037), histone H3 (4499), phospho-PKC (pan; 9371), PKCζ (9368), cleaved PARP (5625), DNA-PKcs (4602), phospho-ATM/ATR substrate (2851), phospho-p53 Ser15 (9286), GST (2622), rabbit antibody to HA (3724) and Myc-tag (2278), and mouse antibody to Myc-tag (2276) were purchased from Cell Signaling Technology. Mouse monoclonal antibody to HA (901503) was purchased from BioLegend. Antibodies to mouse IgG (A4416), rabbit IgG (A4914), rabbit-anti-Flag (F7425) and mouse-anti-Flag (F3165) were purchased from Sigma Aldrich. Antibodies to α-tubulin (66031–1-Ig) and PARP1 (13371–1-AP) were purchased from Proteintech. Antibodies to ECT2 (sc-1005, discontinued) and LIG4 (sc-28232, discontinued) were purchased from Santa Cruz Biotechnology. Antibody to BRCA1 (MABC 199) was purchased from EMD Millipore. Antibody to 53BP1 (612523) was purchased from BD Biosciences.
Liu L., Dai X., Yin S., Liu P., Hill E.G., Wei W, & Gan W. (2022). DNA-PK promotes activation of the survival kinase AKT in response to DNA damage through an mTORC2–ECT2 pathway. Science signaling, 15(715), eabh2290.
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3

Apoptosis and PI3K/AKT/mTOR Pathway Modulation

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5-FU, ACP-196 and MK-2206 were purchased from Selleck. HZ-A-018 was provided by Hangzhou Hezheng Pharmaceutical Co. Ltd. RPMI-1640 medium and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, United States). The Annexin V-FITC Apoptosis Kit was purchased from BestBio (Shanghai, China). The primary antibodies against poly (ADP-ribose) polymerase (PARP), cleaved PARP, procaspase-3, cleaved caspase-3, PI3 Kinase p110α, phospho-mTOR (Ser2448), mTOR, phospho-AKT (Ser 473), AKT, Phospho-p70 S6 Kinase (Thr421/Ser424), p70 S6 Kinase, phospho-S6 Ribosomal Protein (Ser240/244), phospho-S6 Ribosomal Protein (Ser235/236), S6 Ribosomal Protein, RRM2, E2F1 were purchased from Cell Signaling Technology (Beverly, MA, United States). The primary antibodies against BTK (phospho Y223), Mcl-1, Bim and β-actin were purchased from Abcam Inc. (Cambridge, MA, United States). The primary antibodies against GAPDH were purchased from Proteintech (Chicago, United States).
Liu D., Xu W., Lin B., Ji C., Shen M., Shen S., Ma J., Zhou X., Yan Y., Zhang B, & Lin N. (2023). HZ-A-018, a novel inhibitor of Bruton tyrosine kinase, exerts anti-cancer activity and sensitizes 5-FU in gastric cancer cells. Frontiers in Pharmacology, 14, 1142127.
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