Cfx connect 180 real time cycler

Manufactured by Bio-Rad

The CFX Connect™ 180 Real‐Time cycler is a compact, flexible, and high-performance real-time PCR system designed for a wide range of applications. It features a thermal cycler with 180 sample capacity, four-color LED-based excitation and detection, and advanced optical and thermal control technologies to ensure accurate and reproducible results.

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Lab products found in correlation

Maxima first strand cdna synthesis kit, by Thermo Fisher Scientific (4 mentions) Sensifast sybr hi rox mix, by Meridian Bioscience (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions)

2 protocols using cfx connect 180 real time cycler

RNA Extraction and qPCR Analysis

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RNA was extracted by standard procedure with TRIzol reagent (Invitrogen). Equal amounts from each sample (500–800 ng) were used for cDNA syntheses applying the Maxima first strand cDNA synthesis kit (Thermo Fisher Scientific). Real‐time qPCR was performed on a CFX Connect^™ 180 Real‐Time cycler (Biorad) (denaturation step: 95°C for 25 sec, annealing and elongation step: 60°C for 30 sec) using SensiFast SYBR^™ Hi‐ROX mix (Bioline) with the following primers:

Zielonka M., Kölker S., Gleich F., Stützenberger N., Nagamani S.C., Gropman A.L., Hoffmann G.F., Garbade S.F, & Posset R. (2019). Early prediction of phenotypic severity in Citrullinemia Type 1. Annals of Clinical and Translational Neurology, 6(9), 1858-1871.

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RNA Extraction and qPCR Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was extracted with TRIzol reagent (Invitrogen) by standard procedure as previously described (Zielonka, Kolker, et al., 2019 (link)). Briefly, equal amounts from each sample (500 to 800 ng) were used for cDNA syntheses using the Maxima first strand cDNA synthesis kit (Thermo Fisher Scientific). Real-time qPCR was performed on a CFX Connect™ 180 Real-Time cycler (Biorad) (denaturation step: 95°C for 25s, annealing and elongation step: 60°C for 30s) using SensiFast SYBR™ Hi-ROX mix (Bioline) with the following primers:

Zielonka M., Garbade S.F., Gleich F., Okun J.G., Nagamani S.C., Gropman A.L., Hoffmann G.F., Kölker S, & Posset R. (2020). From genotype to phenotype: Early prediction of disease severity in argininosuccinic aciduria. Human mutation, 41(5), 946-960.

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