Gapdh antibody g 9

Western blotting was performed as we described previously [26 (link)–29 (link)]. GAPDH was used to standardize cytosolic protein loading on the sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Mcl-1 antibody (S-19) (sc-819), c-Myc antibody (9E10) (sc-40), cyclin D1 antibody (H-295) (sc-753), VEGF antibody (147) (sc-507), LRP5 antibody (B-9) (sc-390267), frizzled-4 antibody (H-120) (sc-135108), p-GSK-3α/β antibody (6D3) (sc-81496), Wnt-5α/β antibody ( sc-376249), Bcl-x_L antibody (H-5) ( sc_-8392), TSPAN12 (sc-133810), β-catenin antibody (H-102) (sc-7199), Bax antibody (N-20): sc-493, p-Akt1/2/3 antibody (Ser 473)-R (sc-7985-R), GAPDH antibody (G-9) (sc-365062), and caspase-3 p11 antibody (C-6) (sc-271759) were purchased from Santa Cruz Biotech (Santa Cruz, Calif). The secondary antibodies were horseradish peroxidase-conjugated goat antimouse immunoglobulin (Ig) G (ICN Biomedicals, Aurora, Ohio) and horseradish peroxidase-conjugated goat antirabbit IgG (ICN Biomedicals).

Custom rat antibody was made commercially (Biosynthesis, Inc., Lewisville, TX) against the synthetic peptide KRNQEINQLISPRPSTSLNS of PIV3 nonstructural protein C as described previously (Rabbani et al., 2016 (link)). Commercial primary antibodies used in immunoblotting included PIV3 HN antibody (Abcam; cat# ab49756), IDO antibody (Santa Cruz; cat# sc-25809), mouse glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (G-9; Santa Cruz; cat# sc-365062). The corresponding horseradish peroxidase-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology: goat anti-mouse IgG (cat# sc-2031), goat anti-rabbit IgG (cat# sc-2030), and goat anti-rat IgG (cat# sc-2032). Lysis of cells, SDS-PAGE and immunoblotting were also performed as described (Rabbani et al., 2016 (link)).