Male Swiss CD-1 mice (Janvier, Le Genest, France) weighing 30–35 g (6–8 weeks old) were randomly assigned to an experimental group. The animals were housed (seven per cage) in polycarbonate cages with wood-derived bedding, in temperature-controlled conditions (22 ± 1 °C) under a 12 h light/dark cycle and had ad libitum access to food (standard laboratory diet, Panlab SL, Barcelona, Spain) and drinking water. All animal care and experimental protocols in this study were approved by the Animal Ethics Committee of the University of Barcelona under the supervision of the Autonomous Government of Catalonia and are in accordance with the guidelines of the European Community Council (2010/63/EU), as amended by Regulation (EU) 2019/1010. Efforts were made to minimize animal use and suffering. All studies involving animals comply with the ARRIVE guidelines [59 (link)]. The animals were supervised before and throughout the study, immediately after injection, and during the behavioral experiment as well as 24 h after injection, and different parameters were visually evaluated for humane endpoints, such as abnormal posture, greater loss than 20%, self-mutilations, and strange vocalizations.
Male swiss cd 1 mice
Manufactured by Janvier Labs
Sourced in France
Male Swiss CD-1 mice are a commonly used laboratory mouse strain. They are outbred, meaning they have a genetically diverse population. These mice are often used in a variety of research applications.
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Lab products found in correlation
2 protocols using male swiss cd 1 mice
1
Swiss CD-1 Mice Welfare Protocol
2
Behavioral Experiments with Swiss CD-1 Mice
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Male Swiss CD-1 mice (Janvier, Le Genest, France) weighing 30-35 g (6-8 weeks old) were used for the behavioral experiments. The animals were housed in polycarbonate cages with wood-derived bedding and temperature-controlled conditions (22 ± 1 °C) under a 12 h light/dark cycle and had free access to food and drinking water. All the studies were carried out following the ARRIVE guidelines [45] . Experimental groups were randomized (block randomization) and researchers were blinded to the group allocation, the outcome assessment and the data analysis. Animal care and experimental protocols were approved by the Animal Ethics Committee of the University of Barcelona under the supervision of the Autonomous Government of Catalonia, in accordance with the guidelines of the European Community Council (2010/63/EU). The sample size was determined using GPower software. The minimal significance was set at 0.05 and statistical power at 0.8.
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