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Pgl3 basic firefly luciferase vector

Manufactured by Thermo Fisher Scientific
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The PGL3 basic firefly luciferase vector is a plasmid used for the expression of firefly luciferase in cells. It contains the firefly luciferase gene under the control of a basic promoter.

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Lab products found in correlation

Veritas, by Promega (2 mentions) Reporter lysis buffer, by Promega (2 mentions)

Spelling variants of this product

PGL3-basic vector (24 citations) PGL3-Basic (17 citations) PGL3 vector (14 citations) PGL3-basic luciferase vector (6 citations) PGL3 luciferase vector (6 citations) Firefly luciferase (6 citations)

2 protocols using pgl3 basic firefly luciferase vector

1

Human Cyclin A Promoter Luciferase Assay

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The human cyclin A promoter (containing 1 GC box and 1 TATAAT box) was cloned into the pGL3 basic firefly luciferase vector (Thermo Scientific) and then transfected into PFDN1-overexpressed cells. The specific primers for promoter sequences are listed in supplementary Table S5. Cell lysates were prepared 24 h after transfection in reporter lysis buffer (Promega, Madison, WI, USA) and luciferase substrate was added. The luciferase activity was recorded on a luminometer (Veritas, Promega).
Wang D., Shi W., Tang Y., Liu Y., He K., Hu Y., Li J., Yang Y, & Song J. (2016). Prefoldin 1 promotes EMT and lung cancer progression by suppressing cyclin A expression. Oncogene, 36(7), 885-898.
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2

Homo Cdh1 Promoter Luciferase Assay

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The Homo Cdh1 wt, mut1, mut2, and mut1-2 promoter sequences were cloned into the pGL3 basic firefly luciferase vector (Thermo Scientific) and subsequently transfected into 293T cells. Following a 24-h incubation period, cell lysates were prepared in reporter lysis buffer (Promega, Madison, WI, USA) and luciferase substrate was introduced. The resulting luciferase activity was measured using a luminometer (Veritas, Promega).
Wang D., Yang Y., Cao Y., Meng M., Wang X., Zhang Z., Fu W., Duan S, & Tang L. (2023). Histone deacetylase inhibitors inhibit lung adenocarcinoma metastasis via HDAC2/YY1 mediated downregulation of Cdh1. Scientific Reports, 13, 12069.
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