Cervical samples assumed as positive for HPV-DNA or presenting cytological abnormalities were evaluated using the PapilloCheck® Test Kit (Greiner Bio-One GmbH, Frickenhausen, Germany), according to the manufacturer’s instructions. This chip-based assay identifies 18 HPV genotypes classified as high-risk (HR) or probable HR-HPV (16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 70, 73, 82) and 6 low-risk (LR) HPV (HPV6, 11, 40, 42, 43, 44/55). The test cannot distinguish between HPV-55 and HPV-44 because of a cross-reaction, and the manufacturer instructions do not differentiate HR-HPV from probable HR-HPV. Slide scanning was performed using a Greiner Bio-One CheckScanner™. Data analysis was performed using CheckReport™ Software. Since PapilloCheck® requires highly pure and concentrated DNA (400–800 ng input), all samples that did not fulfill the criteria were excluded from the study.
Papillocheck test kit
Manufactured by Greiner
Sourced in Germany
The PapilloCheck® Test Kit is a laboratory equipment product designed for the detection and identification of human papillomavirus (HPV) DNA. It provides a standardized and reliable method for the analysis of HPV in clinical samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using papillocheck test kit
1
HPV Genotyping with PapilloCheck® Test
2
HPV DNA Extraction and Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On reception at the HPV National Reference Center, 16 mL of cytologic sample was transferred into a 50-mL Falcon tube and centrifuged at 4500 Â g for 10 minutes. The supernatant was removed, and the pellet was washed with 1 mL of phosphate-buffered saline. Sample was then centrifuged again at 5000 Â g for 10 minutes, and the supernatant was removed. The pellet was frozen at À80 C before DNA extraction. After DNA extraction (Macherey Nagel, Düren, Germany), HPV was detected using the PapilloCheck Test Kit (Greiner Bio-One GmbH, Frickenhausen, Germany), according to the manufacturer's instructions (Supplemental Table S2).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!