CMD sodium salt (Mw = 10,000–20,000 Da), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide·hydrochloride (EDC·HCl), N-hydroxysulfosuccinimide (NHS), dopamine·HCl, and anatase TiO2 NPs (25 nm in diameter) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The NIR dye, FCR-675 amine, was purchased from BioActs (Incheon, Korea). Cy5.5 was purchased from Amersham Biosciences (NJ, USA). Singlet oxygen sensor green reagent was purchased from Life Technologies Korea LLC (Seoul, Korea). The ELISA complete kit for mouse IL-6, IL-1β, and TNF-α was purchased from Komabiotech (Seoul, Korea). All other reagents were analytical grade. SCC7 and NIH3T3 cell lines were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). For cell culture, RPMI-1640, DMEM, trypsin-EDTA, and fetal bovine serum (FBS) were purchased from Welgene Inc. (Daegu, Korea). All experimental involving live animals were carried out in accordance with the relevant laws and institutional guidelines of Sungkyunkwan University. The Sungkyunkwan University institutional committees have approved all the experimental protocols.
Cmd sodium salt
Manufactured by Merck Group
Sourced in United States
CMD sodium salt is a laboratory reagent commonly used as a dispersing agent and stabilizer in various scientific applications. It is a white, crystalline powder that is soluble in water. CMD sodium salt is frequently utilized in the preparation of buffer solutions and as a component in analytical methods, though its specific intended uses should be determined by the end-user.
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Lab products found in correlation
Trypsin edta, by Welgene (2 mentions)
Anatase tio2 nps, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Rpmi 1640, by Welgene (1 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)
Sodium cyanoborohydride, by Tokyo Chemical Industry (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc hcl, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Dulbecco s pbs dpbs, by Welgene (1 mentions)
Methoxy polyethylene glycol amine mpeg nh2, by Laysan Bio (1 mentions)
Antibiotic antimycotic solution, by Welgene (1 mentions)
Rpmi 1640 media, by Welgene (1 mentions)
Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Sodium pyrophosphate, by Merck Group (1 mentions)
Sodium phosphate, by Merck Group (1 mentions)
Coomassie brilliant blue, by Merck Group (1 mentions)
Iron 2 chloride tetrahydrate, by Merck Group (1 mentions)
β nicotinamide adenine dinucleotide β nad, by Merck Group (1 mentions)
Adh from s cerevisiae, by Merck Group (1 mentions)
5 protocols using cmd sodium salt
1
Synthesis and Characterization of NIR-activatable Theranostic Nanoparticles
2
Synthesis and Characterization of mPEG-Ce6 Conjugates
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Methoxy polyethylene glycol amine [mPEG-NH2, molecular weight (Mw) = 5 kDa] was purchased from Laysan Bio Inc. (Alabama, USA). TCPO and sodium cyanoborohydride were purchased from Tokyo Chemical Industry (Tokyo, Japan). CMD sodium salt (Mw = 10 to 20 kDa), N-(3-dimethylaminopropyl)-N′-ethyl carbodiimide hydrochloride (EDC•HCl), 4-(dimethyl amino)pyridine (DMAP), dimethyl sulfoxide (DMSO), dimethyl formamide (DMF), 2′,7′-dichlorofluorescin diacetate (DCF-DA), TMP, DMPO, and [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The Ce6 was obtained from Frontier Scientific Inc. (Logan, Utah, USA). Human colorectal adenocarcinoma cells (HT29) were purchased from the American Type Culture Collection (Rockville, MD, USA). For cell culture, RPMI 1640 media, antibiotic-antimycotic solution, trypsin-EDTA, and Dulbecco’s PBS (DPBS) were purchased from Welgene Inc. (Daegu, Korea). All experiments involving live animals were carried out in accordance with the relevant laws and institutional guidelines of Sungkyunkwan University.
3
Conjugation of CMD Sodium Salt with OVA
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CMD sodium salt (Mn = 10,000–20,000 Da), OVA, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide·hydrochloride (EDC·HCl), N-hydroxysuccinimide (NHS), sodium cyanoborohydride (NaBH3CN), and fluorescein isothiocyanate (FITC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The near-infrared fluorescent (NIRF) probe, cyanine 5.5 (Cy5.5), was purchased from Amersham Bioscience (Piscataway, NJ, USA). The water used in this study was prepared by an AquaMax-Ultra Water Purification System (Anyang, Republic of Korea). All other chemicals were prepared by commercial sources, and they were used as received without purification.
4
Spectrophotometric Assay of Alcohol Dehydrogenase
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Iron (III) chloride hexahydrate (FeCl3∙6H2O), iron (II) chloride tetrahydrate (FeCl2∙4H2O) and Coomassie brilliant blue were obtained from Merck. Ammonium hydroxide was purchased from Chem-Lab, Belgium. CMD sodium salt, EClH (1-Chloro-2,3-epoxypropane), sodium pyrophosphate, sodium phosphate, ethanol, β-nicotinamide adenine dinucleotide (β-NAD) and ADH from S. cerevisiae were purchased from Sigma-Aldrich. All reagents in this work were of analytical purity and used without further purification. In all experiments, deionized water was used.
5
Chitosan-Carboxymethyl Dextran Hydrogel
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Cs medium-molecular-weight Chi with a degree of deacetylation of about 89% was purchased from Primex (Karmoy, Norway). TPH was supplied by Yangzhou Huaxing Chemical Company, China. CMD sodium salt (10–20 KD, 1.1–1.5 mmol carboxyl/g), dialysis tubing (molecular weight cut-off 2 and 12 kDa), N-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), Ellman's reagent, RPMI-1640 tissue culture medium, and fetal bovine serum (FBS) were purchased from Sigma-Aldrich Company (Missouri, USA). N-methyl-2-pyrrolidone (NMP) and hydrochloric acid (HCl), sodium chloride, and sodium hydroxide (NaOH) were purchased from Merck Company (Darmstadt, Germany). The human Rb cell line (Y79) was obtained from Pasteur Institute (Tehran, Iran) and cell-counting solution (Orangu TM) was prepared by Cambridge Bioscience. All chemicals were of analytical grade.
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