2 7 dichlorofluorescin diacetate dcfda cellular ros detection assay kit

Manufactured by Abcam

Sourced in United Kingdom, Australia

2',7'-dichlorofluorescin diacetate (DCFDA) Cellular ROS Detection Assay Kit is a fluorogenic dye that measures hydroxyl, peroxyl, and other reactive oxygen species (ROS) activity within a cell. The non-fluorescent DCFDA is converted to a fluorescent compound upon oxidation.

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Lab products found in correlation

Trypan blue dye, by Merck Group (1 mentions) Pcb 153, by Merck Group (1 mentions) Trypsin edta solution, by Thermo Fisher Scientific (1 mentions) Muse annexin 5 dead cell kit, by Merck Group (1 mentions) Cho k1, by American Type Culture Collection (1 mentions) N acetyl l cysteine nac, by Merck Group (1 mentions) Apc conjugated annexin 5, by BioLegend (1 mentions) M csf, by Cell Signaling Technology (1 mentions) Rpmi 1640, by Corning (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Comet assay kit, by Bio-Techne (1 mentions)

Spelling variants of this product

DCFDA Cellular ROS Detection Assay Kit (178 citations) Cellular ROS Assay Kit (92 citations) Cellular ROS Detection Assay Kit (50 citations) DCFDA Cellular ROS Assay Kit (27 citations) DCFDA cellular ROS detection assay (14 citations) DCFDA Cellular ROS Detection Kit (12 citations) 2′,7’-dichlorofluorescin diacetate (DCFDA) (9 citations) Cellular ROS detection assay (4 citations) Cellular ROS Detection Kit (4 citations) 2′,7′‐dichlorofluorescin diacetate (2 citations)

4 protocols using 2 7 dichlorofluorescin diacetate dcfda cellular ros detection assay kit

Cytotoxicity and Cellular Oxidative Stress in CHO-K1 Cells Exposed to PCB 153

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The Chinese hamster ovary cell line (CHO-K1) was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Dulbecco’s MEM F-12 (DMEM F-12), heat inactivated foetal bovine serum (FBS), and trypsin/EDTA solution (0.25 % trypsin with EDTA 4Na) were supplied by GIBCO (Paisley, UK). PCB 153 (2,2’,4,4’,5,5’-hexachlorobiphenyl, CAS 35065-27-1), Trypan Blue dye (CAS 72-57-1), MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 h-tetrazolium bromide, CAS 298-93-1), and propidium iodide (PI, CAS 25535-16-4) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Coomassie brilliant blue R-250 (CAS 6104-59-2) was purchased from LKB (Bromma, Sweden). Neutral Red dye (CAS 553-24-2) and Muse™ Annexin V & Dead Cell Kit (cat #MCH100105) were supplied by Merck (Billerica, MA, USA). The 2’,7’-dichlorofluorescin diacetate (DCFDA) Cellular ROS Detection Assay Kit (cat #ab113851) was obtained from Abcam (Cambridge, UK). DMSO (CAS 67-68-5) and ethanol (CAS 64-17-5) were purchased from Kemika (Zagreb, Croatia).

Miletić M., Murati T., Šimić B., Bilandžić N., Brozović A, & Kmetič I. (2021). Ortho-substituted PCB 153: effects in CHO-K1 cells. Archives of Industrial Hygiene and Toxicology, 72(4), 326-332.

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Oxidative Stress Detection Assay

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All chemicals and reagents used in the study were obtained from Sigma-Aldrich (Sydney, Australia), unless otherwise specified. The 2′,7′-dichlorofluorescin diacetate (DCFDA) cellular ROS detection assay kit was purchased from Abcam (Melbourne, Australia).

Figueroa Gonzalez D, & Young F. (2020). Gamma Tocopherol Reduced Chemotherapeutic-Induced ROS in an Ovarian Granulosa Cell Line, But Not in Breast Cancer Cell Lines In Vitro. Antioxidants, 9(1), 51.

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Fatty Acid Modulation of Macrophage ROS

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M-CSF was purchased from Cell Signaling Technology (Danvers, MA). PA (S-1109, 5 mM), OA (S-1120, 5 mM), LA (S-1127, 5 mM), EPA (S-1144, 5 mM), and DPA (DPA n-3, S-1145, 5 mM) were purchased from Nu-Chek Prep and prepared with 2 mM of endotoxin-free BSA (Akron) in PBS, sonicated until dissolved, and filtered through 0.22 μm sterile filter as we previously described (19 (link)-22 ). For the cell culture, RPMI 1640 (Cellgro, 15–040-cv) was from Corning and Fetal Bovine Serum (FBS, S11550) from Atlanta Biologicals. ROS generation was detected using the DCFDA (2’,7’-dichlorofluorescin diacetate) cellular ROS detection assay kit (ab113851) from Abcam. The ROS inhibitor N-acetyl-L-cysteine (NAC, A7250) was purchased from Sigma-Aldrich. APC conjugated Annexin-V (640920) was purchased from Biolegend. Antibodies used for flow cytometry were obtained from Biolegend and BD Bioscience.

Liu L., Jin R., Hao J., Zeng J., Yin D., Yi Y., Zhu M., Mandal A., Hua Y., Ng C.K., Egilmez N.K., Sauter E.R, & Li B. (2020). Consumption of the fish oil high-fat diet uncouples obesity and mammary tumor growth through induction of reactive oxygen species in pro-tumor macrophages. Cancer research, 80(12), 2564-2574.

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Cellular ROS Detection and DNA Damage

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DMEM culture media were used with 10% FBS (fetal bovine serum) and 1% antibiotics. The DCFDA (2′,7′-dichlorofluorescin diacetate) cellular ROS detection assay kit for a microplate assay was purchased from Abcam. The comet assay kit was purchased from Trevigen. 2,9-Diphenyl-1,10-phenanthroline was synthesized according to a published procedure. 35 The remaining ligands, salts, and solvents (HPLC grade) were purchased from Aldrich and used without further purification.

Al Hageh C., Al Assaad M., El Masri Z., Samaan N., El-Sibai M., Khalil C, & Khnayzer R.S. (2018). A long-lived cuprous bis-phenanthroline complex for the photodynamic therapy of cancer. Dalton transactions (Cambridge, England : 2003), 47(14).

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