A CO2 Rayjet laser engraver was used to fabricate the structures. A customized setup was used for reflection efficiency measurements, comprising a hot plate, a bifurcated optical fiber purchased from Thorlabs, USB2000+ miniature spectrometer for VIS-NIR measurements and a tungsten halogen light source, both purchased from Ocean Optics, an infrared thermometer and a computer with a dedicated software for spectral acquisition. Imaging was performed using a Zeiss optical microscope. The optical fiber was kept firm by a probe holder, to provide irradiation and detection at normal incidence (90°). One probe was connected to a tungsten halogen light source to irradiate the lens, whilst the reflectance probe was connected to a miniaturized optical detector. The detector was connected to a computer with a dedicated software for spectral acquisition.
Usb2000 miniature spectrometer
Manufactured by OceanOptics
The USB2000+ miniature spectrometer is a compact, high-performance optical instrument designed for a wide range of spectroscopic applications. It features a high-resolution CCD detector, a robust optical bench, and a USB interface for easy connectivity to a computer. The device is capable of measuring the intensity of light over a specific wavelength range, providing users with detailed spectral data.
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Lab products found in correlation
3 protocols using usb2000 miniature spectrometer
1
Laser Fabricated Optical Structures
2
Optical Characterization of Functional Materials
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Absorption measurements were performed using a Hewlett-Packard 8452A Diode Array spectrophotometer, using a cuvette filled with pure solvent as the blank. For PL spectroscopy, we used the 405-nm ion line of a diode laser operated at an excitation power density of ~1250Wm-2 as the excitation source. The PL was collected by an optical fiber (numerical aperture ~0.22) and directed to an Ocean Optics USB 2000+ miniature spectrometer. The efficiency of the system was corrected with an Ocean Optics calibrated light source. Photographs of the PE layers were taken with a Canon Rebel T3i camera.
3
Biofluorescence Emission Spectroscopy
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Emission spectra were collected using an Ocean Optics USB2000+ miniature spectrometer (Dunedin, FL) equipped with a hand-held fiber optic probe (Ocean Optics ZFQ-12135). Excitation spectra were achieved during illumination with a band-pass filter (450–500 nm, Omega Optical, Inc., Brattleboro, VT, or Semrock, Inc., Rochester, NY). Emission spectra were recorded by applying the fiber optic probe to specific anatomical parts of the individual fish specimen exhibiting biofluorescence. This was repeated several times for each specimen to ensure the accuracy of measurements.
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