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7 protocols using tetrachloroauric acid haucl4 3h2o

1

Synthesis of Gold Nanoparticles

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Cetyltrimethyl ammonium bromide (CTAB), sodium borohydride (NaBH4), tetraethyl orthosilicate (TEOS), ascorbic acid, PEGDE (molecular weight ~ 526), GAL, dimethyl sulfoxide (DMSO), ethanol, methanol, and tetrachloroauric acid (HAuCl4·3H2O) were purchased from Sigma-Aldrich (St. Louis, MO, USA). ECH (purity: ≥ 99.85%) was purchased from MedChemExpress (Monmouth Junction, NJ, USA; HY-N0020).
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2

Synthesis of Colloidal Nanocrystals

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Cesium
carbonate (Cs2CO3), lead bromide (PbBr2), 1-octadecene(ODE), oleic acid
(OA), and oleylamine (OLAM) were purchased from Sigma-Aldrich and
used without any further purification. Tetrachloroauric acid (HAuCl4·3H2O) was purchased from Sigma-Aldrich. Sodium
borohydride (NaBH4), sodium hydroxide (NaOH), tetraoctyl
ammonium bromide (TOABr), and 2-phenylethanethiol (PhC2H4SH, 99%) were purchased from Merck India Ltd. Methyl
acetate (MeOAC, Merck) was used during the purification of the nanocrystals.
Solvents like toluene, ethanol, methanol, acetonitrile, and dichloromethane
were obtained from Spectrochem. toluene (Spectrochem) was used for
all spectroscopic experiments, including the characterization of the
nanocrystals.
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3

Antimicrobial Potential of Gold Nanoparticles

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D. radiodurans R1 (ATCC13939) was grown aerobically at 32°C in tryptone glucose yeast (TGY) broth (0.5% tryptone, 0.1% glucose, 0.3% yeast extract) with agitation at 220 rpm. E. coli and S. aureus were grown at 37°C in Luria–Bertani (LB) medium (1% NaCl, 0.5% yeast extract and 1% tryp-tone). Bacterial growth was assessed by measuring optical density (OD) at 600 nm for D. radiodurans and E. coli and OD at 590 nm for S. aureus. Tetrachloroauric acid (HAuCl4·3H2O) was purchased from Sigma Aldrich Co. (St Louis, MO, USA). All reagents used were of analytical grade. A gold(III) stock solution was obtained by dissolving HAuCl4 in ultrapure water. The gold(III) solutions used in this study were prepared by dilution of a 10 mM HAuCl4 stock solution. The pH of each working solution was adjusted by hydrochloric acid or sodium hydroxide. Chloramphenicol was used as a control in the antibacterial assays.
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4

Synthesis of Gold Nanoparticles via Citrate Reduction

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Synthesis of AuNPs was carried out according to the procedures described in the citrate reduction method [17 (link),18 (link)]. Briefly, 0.0394 g of tetrachloroauric acid (HAuCl4·3H2O) (Sigma Aldrich Chemical Company, Atlanta, GA, USA) was dissolved in 100 mL of nanopure water (18 MΩ of resistance) in a three-neck round flask (1 mM HAuCl4) connected to reflux condenser. The resulting solution was isovolumetrically heated to boiling point under stirring and refluxed. Then, 10 mL of a 38.8 mM trisodium citrate solution was preheated to 60 °C and quickly added to the boiling solution of HAuCl4 under vigorous stirring. After the solution turned from pale yellow to black and to deep red, it was refluxed for additional 30 min and subsequently cooled to room temperature without stirring for at least 2 h. The formed nanoparticle suspension was filtered through Millipore Nylon filter (0.45 µm) and preserved in the dark at 4 °C for subsequent pH determination and characterization of the AuNPs.
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5

Synthesis of Gold Nanoparticles with PEG Functionalization

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Dacarbazine (C6H10N6O) ≥ 99%, molar weight: 182.18 g/mol; β-cyclodextrin (C42H70O35) ≥ 97%, molar weight: 1134.98 g/mol; tetrachloroauric acid (HAuCl4*3H2O) ≥ 99.9%, molar weight: 393.83 g/mol; sodium citrate (Na3C6H5O7) ≥ 99%, molar weight: 294.10 g/mol; and nitric acid (HNO3) 70%, molar weight: 63.01 g/mol were provided by Sigma Aldrich (Saint Louis, MO, USA). Hydrochloric acid (HCl) 37%, molar weight: 36.46 g/mol; ethanol (C2H5OH) ≥ 99.9%, molar weight: 46.07 g/mol; hexane (C6H14) ≥ 99.7%, molar weight: 86.18 g/mol; and water (nanopure) were provided by Merck (Darmstadt, Germany). Methoxy PEG Thiol (CH3O-PEG5000-SH) ≥ 95%, molar weight: 5 kDa was provided by JenKem Technology (Plano, TX, USA).
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6

Functionalized gold nanoparticles for bioassays

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Sodium borohydride (NaBH4), 1-ethyl-3-(3-dimethly-aminopropyl) carbodiimide (EDC), tetraethyl orthosilicate (TEOS), ascorbic acid, sodium hydroxide, concentrated sulfuric acid, hydrochloric acid (12 mol/L), ethanol, (3-aminopropyl) triethoxysilane (APTES), cetyltrimethyl ammonium bromide (CTAB), N-hydroxysuccinimide (NHS), and tetrachloroauric acid (HAuCl4·3H2O) were bought from Sigma-Aldrich (St. Louis, MO, USA). We purchased HM from ChemeGen (Los Angeles, CA, USA); Asp6 was purchased from Shanghai Botai Biotechnology Co., Ltd. (Shanghai, China).
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7

Preparation of Aqueous Tetrachloroauric Acid

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Tetrachloroauric acid (HAuCl4·3H2O) was obtained from Sigma-Aldrich (Saint Louis, MO, USA). An aqueous solution of HAuCl4 (1 mM) freshly prepared in double-distilled (DD) water was used throughout the experimental work reported here. All glassware used in this work was thoroughly rinsed with pure water before starting.
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