Ginkgolide C (GC, Purity ≥98%, PubChem CID: 161120) was purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). Zinc (II) Protoporphyrin IX (ZnPP), protease Inhibitor and ML385 were purchased from MCE (MedChemExpress, United States). Toluidine blue and 4% paraformaldehyde were purchased from Solarbio (Beijing, China). Type II Collagenase and 0.25% Trypsin-EDTA were purchased from Gibco (United States). Penicillin/streptomycin, CCK-8, DAPI, and fluorescent probe DCFH-DA were purchased from Beyotime (Shanghai, China). ProteinTech (Wuhan, China) provided the main antibodies for MMP3, IκBα, GAPDH, HO-1, and Nrf2. p65, Bcl-2, SOX9, and cleaved-caspase3 (C-caspase3) antibodies were bought from Cell Signaling Technology (Danvers, United States). Antibodies for Bax, iNOS, MMP13, Nrf2, p-IκBα, and Lamin B were bought from Affinity (Jiangsu, China). Antibodies for COX-2 and ADAMTS4 were acquired from ABclonal (Wuhan, China). OriGene (Wuxi, China) provided the β-actin antibody. Alexa 488 Goat Anti-Rabbit IgG (H + L) was purchased from APExBIO (Houston, United States).
Mmp13
Manufactured by Affinity Biosciences
Sourced in China, United States
MMP13 is a laboratory reagent used for the detection and quantification of Matrix Metalloproteinase 13 (MMP13), a member of the matrix metalloproteinase family of enzymes. MMP13 plays a role in the breakdown of extracellular matrix components and is involved in various physiological and pathological processes.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Beyotime (2 mentions)
Adcy7, by Thermo Fisher Scientific (2 mentions)
Il 1β, by Affinity Biosciences (2 mentions)
Collagenase type 2, by Thermo Fisher Scientific (1 mentions)
Cleaved caspase 3 c caspase 3, by Cell Signaling Technology (1 mentions)
Dcfh da fluorescent probe, by Beyotime (1 mentions)
P iκbα, by Affinity Biosciences (1 mentions)
Lamin b, by Affinity Biosciences (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Cck 8, by Beyotime (1 mentions)
Penicillin streptomycin, by Beyotime (1 mentions)
Gapdh, by Proteintech (1 mentions)
Toluidine blue, by Solarbio (1 mentions)
Paraformaldehyde (pfa), by Solarbio (1 mentions)
Polyvinylidene fluoride (pvdf), by Merck Group (1 mentions)
E cadherin, by Affinity Biosciences (1 mentions)
Vimentin, by Affinity Biosciences (1 mentions)
Sds page, by Biosharp (1 mentions)
Plin1, by Abcam (1 mentions)
5 protocols using mmp13
1
Ginkgolide C Modulates Oxidative Stress and Inflammation
2
Western Blot Analysis of Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total proteins obtained from cells and tissues were subjected to SDS–PAGE (Biosharp, China) and then transferred to PVDF (Millipore, USA) membranes and blocked in 5% skimmed milk for 1 h. The membranes were incubated overnight at 4 °C with primary antibodies against ADCY7 (Thermo Fisher, USA), IL-1β (Affinity, China), MMP-13 (Affinity, China), N-cadherin (Affinity, China), E-cadherin (Affinity, China), vimentin (Affinity, China), α-SMA (CST, USA), p-PKA substrate (CST, USA), p-PLIN1(Abcam, USA), PLIN1 (Abcam, USA), p-HSL (CST, USA) and GAPDH. The following day, the membranes were incubated with fluorophore-conjugated secondary antibody (LI-COR Corp., NE). Protein bands were imaged with an enhanced LI-COR Odyssey infrared imaging system (LI-COR Corp., NE). The protein levels were normalized to the GAPDH levels.
3
Immunofluorescence Analysis of Chondrocyte Spheres
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immunofluorescence, frozen sections of chondrocyte spheres were washed with PBS for three times, these cell slices were permeabilized with cold 0.2% Triton X-100 (Sigma, USA) in PBS for 5 min. A step of enzymatic antigen retrieval with 0.1% Trypsin in PBS was performed prior to block for 1 h. After blocking, antibodies against Aggrecan (1:300; Abcam, UK), COL II (1:300; Abcam), SOX9 (1:200; Affinity), MMP13 (1:200; Affinity), COL X (1:500; Abcam), NANOG (1:300; Abcam), OCT4 (1:300; Abcam), TRA-1-60(1:300; Abcam) and Ki67 (1:300; Abcam) were added overnight. The following day, cells were washed three times with PBS and then incubated at 37 °C for 1 h with Alexa 594-conjugated goat anti-rabbit secondary antibody (1:300; Abcam) or Alexa 488-conjugated goat anti-mouse secondary antibody (1:300; Abcam). The cell nucleus was counterstained with DAPI (Beyotime, People’s Republic of China).
4
Western Blot Protein Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The protocol and procedure for Western blot were performed as described in previous reports [40 (link)]. For each sample, the 30 μg extracted total protein was loaded onto a 10–15% SDS/PAGE gel. The gel-separated protein was then transferred to a PVDF membrane (Millipore) and incubated with the primary antibodies of anti-transforming growth factor-β (TGF-β, Abcam), anti-SMAD2/3 (Abcam), anti-collagen type II (COL II, Abcam), anti-SOX-9 (Abcam), anti-collagen type X (COL X, Abcam), anti-Indian hedgehog (IHH, Affinity, OH, USA), anti-matrix metalloproteinase 13 (MMP 13, Affinity), anti-stem cell-derived factor 1 (SDF-1, Affinity), anti-vascular endothelial growth factor (VEGF, Affinity), and anti-β-actin (Invitrogen) at 37 °C for 2 h, followed by incubation with horseradish peroxidase-conjugated secondary antibodies. Protein expression was visualized, and the values were normalized against β-actin.
5
Comprehensive Osteoarthritis Assessment Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Joint samples were collected and fixed in 4% paraformaldehyde (G-Clone, China) for 48 h, decalcified in 10% EDTA (G-Clone, China) for 4 weeks, embedded in paraffin and cut into sections (5 μm). Immunohistochemistry was performed using the DAB staining method. Briefly, the slides were deparaffinized and rehydrated, followed by antigen retrieval performed using sodium citrate at 95 °C. The slides were then blocked with 3% bovine serum albumin for 30min at room temperature and incubated with primary antibodies against ADCY7 (Thermo Fisher, USA), MMP13 (Affinity, China), IL-1β (Affinity, China), CD86 (a surface marker of M1 macrophages, Abcam, USA), CD55 (surface marker of FLS, Abcam, USA) overnight at 4 °C and a secondary antibody (Affinity, China), or a fluorescent secondary antibody (Affinity, China), for 30 min at 37 °C. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 10 min at room temperature before stained with 3, 30-diaminobenzidine tetra hydrochloride (DAB) and counterstained with hematoxylin. Slices of rat knee joints were also stained with safranin O/fast green (G-Clone, China) and toluidine blue (G-Clone, China). The Osteoarthritis Research Society International (OARSI) scoring system was used to evaluate the OA cartilage pathology [13 (link)]. A synovitis scoring system was used to evaluate the degree of synovitis [14 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!