Polyclonal PIPKIγ anti-serum was generated by immunizing rabbits using purified His-tagged mouse PIPKIγ and purified using a human PIPKIγ-conjugated affinity column to generate the anti-pan-PIPKIγ antibody as described 9 (link). To generate the phosphorylated-PIPKIγ antibody, we immunized rabbits with a PIPKIγ phosphopeptide (CDIpYFPTDERSWVYSPLHYSA). The anti-sera were collected, pre-cleaned by a non-phosphopeptide (CDIYFPTDERSWVYSPLHYSA) affinity column, then purified using an affinity column conjugated with the phosphopeptide. Antibodies: anti-HA and MMP9 antibodies (Millipore, Billerica, MA); pERK1/2 and ERK1/2 antibodies (Cell Signaling, Danvers, MA); β-actin antibody and monoclonal anti-α smooth muscle actin antibody (Sigma, St. Louis, MO); Rabbit anti-Cytokeratin 8 antibody, Rat monoclonal anti-CD34 antibody and monoclonal anti-CD68 antibody (Abcam, Cambridge, MA); Alexa Fluor 488 goat anti-mouse antibody, Alexa Fluor 555 goat anti-rabbit antibody and Alexa Fluor 555 goat anti-rat antibody (Molecular probes).
β actin antibody and monoclonal anti α smooth muscle actin antibody
Manufactured by Merck Group
The β-actin antibody is a primary antibody used for the detection and quantification of β-actin, a ubiquitously expressed cytoskeletal protein. The monoclonal anti-α smooth muscle actin antibody is a primary antibody used for the detection and localization of α-smooth muscle actin, a marker for smooth muscle cells.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 goat anti mouse antibody, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 555 goat anti rabbit antibody, by Thermo Fisher Scientific (2 mentions)
Perk1 2 and erk1 2 antibodies, by Cell Signaling Technology (2 mentions)
Anti ha and mmp9 antibodies, by Merck Group (2 mentions)
Rabbit anti cytokeratin 8 antibody, by Abcam (2 mentions)
Rat monoclonal anti cd34 antibody and monoclonal anti cd68 antibody, by Abcam (2 mentions)
Alexa fluor 555 goat anti rat antibody, by Thermo Fisher Scientific (2 mentions)
2 protocols using β actin antibody and monoclonal anti α smooth muscle actin antibody
1
Generation of Anti-PIPKIγ Antibodies
2
Generation of Anti-PIPKIγ Antibodies
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