TiO2-coated glass slides were created by atomic layer deposition of titanium(IV) isopropoxide on glass; the full procedure has been reported elsewhere [20 (link)]. Verapamil, buspirone, and titanium(IV) oxide nanopowder P25 were obtained from Sigma-Aldrich (Zwijndrecht, The Netherlands). Testosterone, ostarine, and andarine were kindly donated by RIKILT (Wageningen, The Netherlands). Ultrapure water (H2O)—18.2 MΩ × cm−1 at 25 °C—was freshly produced daily with a Millipore (Molsheim, France) Integral 3 system. Acetonitrile (ACN) LC-MS grade was bought from VWR (Leuven, Belgium). Methanol (MeOH) UPLC-MS grade was purchased from Biosolve (Valkenswaard, The Netherlands). Formic acid (FA) LC-MS grade was bought from Fisher Scientific (Geel, Belgium) and leucine-enkephalin (leu-enk) for lock mass-corrected mass calibration was purchased from Waters (Manchester, UK).
Integral 3 system
Manufactured by Merck Group
Sourced in France
The Integral 3 system is a laboratory equipment produced by the Merck Group. It is designed to perform a core function within a laboratory setting. The Integral 3 system provides essential capabilities to support laboratory operations, though a detailed description of its intended use is not available.
Automatically generated - may contain errors
Lab products found in correlation
Lc ms grade formic acid, by Thermo Fisher Scientific (1 mentions)
Verapamil, by Merck Group (1 mentions)
Buspirone, by Merck Group (1 mentions)
Leucine enkephalin leu enk, by Waters Corporation (1 mentions)
Melamine, by Merck Group (1 mentions)
L arginine, by Merck Group (1 mentions)
96 well plate, by Merck Group (1 mentions)
Reserpine, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
2 propanol, by Merck Group (1 mentions)
Bicinchoninic acid protein assay kit, by Merck Group (1 mentions)
Paracetamol, by Merck Group (1 mentions)
Procaine, by Merck Group (1 mentions)
4 protocols using integral 3 system
1
ALD-Coated Glass Slide Preparation
2
NMR Analysis of Organic Compounds
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Ultrapure water (H2O) 18.2 MΩ × cm–1 at 25
°C was freshly produced with a Millipore (Molsheim, France) Integral 3 system.
Deuterium oxide (D2O) 99.9% atom D, methanol-d4(MeOD) 99.8%+ atom D, melamine, andl -arginine were purchased from Sigma-Aldrich
(Zwijndrecht, The Netherlands). A new ampule of D2O was used for each analysis.
An orange was obtained from a local supermarket. An orchid (Phalaenopsis)
was purchased from a local plant store.
°C was freshly produced with a Millipore (Molsheim, France) Integral 3 system.
Deuterium oxide (D2O) 99.9% atom D, methanol-d4(MeOD) 99.8%+ atom D, melamine, and
(Zwijndrecht, The Netherlands). A new ampule of D2O was used for each analysis.
An orange was obtained from a local supermarket. An orchid (Phalaenopsis)
was purchased from a local plant store.
3
Bioorthogonal Reagents for Biomolecule Labeling
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Ultrapure water (H2O), 18.2 MΩ cm–1 at 25 °C, was
freshly produced daily with a Millipore (Molsheim, France) Integral
3 system. Methanol (MeOH), LC–MS grade, was purchased from
VWR (Leuven, Belgium). N-[(1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyloxycarbonyl]-1,8-diamino-3,6-dioxaoctane
(BCN-amine,1 ), trans-4-cycloocten-1-yl(3-aminopropyl)
carbamate (TCO-amine,4 ), 3,6-di-2-pyridyl-1,2,4,5-tetrazine
(dipyridyl-tetrazine,2a ), 3,6-di-2-chlorophenyl-1,2,4,5-tetrazine
(clofentezine,2b ), 3,6-diphenyl-1,2,4,5-tetrazine (diphenyl-tetrazine, 2c ), reserpine, and 96-well plates (untreated) were obtained
from Sigma-Aldrich (Zwijndrecht, The Netherlands). Chemical structures
of reagents are given inFigure 1 . All chemicals were used without further purification.
freshly produced daily with a Millipore (Molsheim, France) Integral
3 system. Methanol (MeOH), LC–MS grade, was purchased from
VWR (Leuven, Belgium). N-[(1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyloxycarbonyl]-1,8-diamino-3,6-dioxaoctane
(BCN-amine,
carbamate (TCO-amine,
(dipyridyl-tetrazine,
(clofentezine,
from Sigma-Aldrich (Zwijndrecht, The Netherlands). Chemical structures
of reagents are given in
4
Standardized Analytical Reagents for Metabolomics
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Methanol (MeOH), chloroform (CHCl3), acetonitrile (ACN), 2-Propanol were of LC-MS grade and has been purchased from Sigma-Aldrich (Darmstadt, Germany), same as Methyl tertiary-butyl ether (MTBE, LC grade).
Ammonium acetate (5 M), ammonium formate (10 M), Bicinchoninic Acid Protein assay Kit were from Sigma-Aldrich (Steinheim, Germany), Ethylsulfate (1 mg/mL), Paracetamol (pharmaceutical primary standard) and Procaine (98%) from Sigma-Aldrich (Taufkirchen, Germany). Acetic acid and formic acid (LC-MS grade) were purchased from Fluka® Analytical (Munich, Germany). Water has been purified using a Merck Millipore Integral 3 system (18.2 MΩ; <3 ppm TOC, Millipore, Germany). Chemical reference standards (Mass Spectrometry Metabolite Library of Standards MSMLS; Bile Acid/Carnitine/Sterol Metabolite Library of Standards BACMLS) have been purchased from Sigma-Aldrich (Taufkirchen, Germany) and have been used according to their respective guidelines.
Ammonium acetate (5 M), ammonium formate (10 M), Bicinchoninic Acid Protein assay Kit were from Sigma-Aldrich (Steinheim, Germany), Ethylsulfate (1 mg/mL), Paracetamol (pharmaceutical primary standard) and Procaine (98%) from Sigma-Aldrich (Taufkirchen, Germany). Acetic acid and formic acid (LC-MS grade) were purchased from Fluka® Analytical (Munich, Germany). Water has been purified using a Merck Millipore Integral 3 system (18.2 MΩ; <3 ppm TOC, Millipore, Germany). Chemical reference standards (Mass Spectrometry Metabolite Library of Standards MSMLS; Bile Acid/Carnitine/Sterol Metabolite Library of Standards BACMLS) have been purchased from Sigma-Aldrich (Taufkirchen, Germany) and have been used according to their respective guidelines.
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