Rabbit anti tace adam17

The following antibodies were used: Goat anti-mouse amphiregulin (R&D Systems, catalogue number AF989; 1:1000), Mouse anti-nonphospho-Tyr1173-EGFR (Millipore, catalogue number 05-484; 1:1000), mouse anti-beta-actin (Santa Cruz, catalogue number sc-47778; 1:2000), rabbit anti-TACE/ADAM17 (Abcam, catalogue number ab39161; 1:2000), rabbit anti-HA (Santa-Cruz, catalogue number sc-805; 1:2000) and mouse anti-transferrin receptor (Invitrogen, catalogue number 13-6800; 1:1000). Corresponding species-specific HRP-coupled secondary antibodies were used from Santa Cruz and Cell Signaling.

The following antibodies were used: mouse anti-beta-actin (Santa Cruz, catalogue number sc-47778; WB 1:2000), rabbit anti-TACE/ADAM17 (Abcam, catalogue number ab39161; WB 1:2000), rabbit anti-HA (Santa-Cruz, catalogue number sc-805; IF 1:1000), mouse anti-HA (ENZO, catalogue number ENZ-ABS120-0200; PM IP 1:1000), mouse anti-transferrin receptor (Invitrogen, catalogue number 13–6800; 1:1000), rabbit anti-phosphoserine (Invitrogen, catalogue number 618100; WB 1:1000), rat HA-HRP, clone 3F10 (Roche, catalogue number 12013819001; WB 1:2000), mouse anti-p97 (Pierce/Thermo, catalogue number MA1-21412; WB 1:1000), rabbit anti-pan14-3-3 (Cell Signalling, catalogue number 8312; WB 1:1000), mouse anti-GM130 (BD Transduction labs, catalogue number 610823; IF 1:1000), rabbit anti-pan-cadherin (Cell Signalling, catalogue number 4068S; WB 1:1000), rabbit anti-ADAM10 (Abcam, catalogue number ab1997; WB 1:1000), rabbit anti-ERK1/2 (Cell Signalling, catalogue number 9102, WB 1:1000), rabbit anti-pERK1/2 (Cell Signalling, catalogue number 4377, WB 1:1000). Corresponding species-specific HRP or fluorescently coupled secondary antibodies were used from Santa Cruz and Cell Signaling (WB) or Invitrogen (IF).