RNA was synthesized using the HiScribe T7 in vitro transcription kit (New England Biolabs). 1 μg of purified PCR product was used for each reaction. Transcription was carried out overnight at 42°C using either ATP or N6-meATP (Trilink). For experiments using varying amounts of m6A within an RNA, transcripts synthesized using all ATP or all N6-meATP were mixed in the indicated proportions.
N6 meatp
Manufactured by TriLink
N6-meATP is a nucleoside triphosphate analogue. It is a modified form of adenosine triphosphate (ATP) where the N6 position of the adenine ring is methylated. N6-meATP can be utilized in various biochemical applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using n6 meatp
1
In vitro RNA Synthesis with m6A
2
In vitro RNA Synthesis with m6A
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