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Cleaved caspase 1 antibody

Manufactured by Cell Signaling Technology
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The Cleaved caspase-1 antibody is a laboratory reagent used to detect the active form of the caspase-1 enzyme. Caspase-1 is a key mediator of inflammatory responses and plays a role in the activation of cytokines. This antibody specifically recognizes the cleaved, active form of caspase-1, which is useful for studying apoptosis and inflammation processes.

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Lab products found in correlation

Nlrc4 antibody, by Merck Group (2 mentions) Pro caspase1 antibody, by Cell Signaling Technology (2 mentions) Hspa8 antibody, by Cell Signaling Technology (2 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Cell Signaling Technology (2 mentions) Ab245190, by Abcam (1 mentions) Ab91526, by Abcam (1 mentions) Nlrp3 antibody, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Cleaved caspase-1 Caspase-1 antibody Caspase-1 Caspases

3 protocols using cleaved caspase 1 antibody

1

NLRC4 and Caspase-1 Activation in THP1 Cells

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5×105 transduced THP1 cells were subjected to SDS-PAGE, transferred to nitrocellulose membranes and probed with appropriate antibodies. Antibodies used for protein blots were NLRC4 antibody (1:500 dilution; Millipore, 06-1125), pro-caspase1 antibody (1:1000 dilution; Cell Signaling Technology, 2225), cleaved caspase-1 antibody (1:500 dilution; Cell Signaling Technologies, 4199), HSPA8 antibody (1:2000 dilution; Cell Signaling Technologies, 8444). Horseradish peroxidase (HRP)-conjugated secondary antibodies (Cell Signaling Technologies, 7074) and SuperSignal West Pico chemiluminescent substrate (Pierce) were used to detect primary antibodies. Equal protein loading was assessed by immunoblotting for HSPA8.
Canna S.W., de Jesus A.A., Gouni S., Brooks S.R., Marrero B., Liu Y., DiMattia M.A., Zaal K.J., Montealegre Sanchez G.A., Kim H., Chapelle D., Plass N., Huang Y., Villarino A.V., Biancotto A., Fleisher T.A., Duncan J.A., O’Shea J.J., Benseler S., Grom A., Deng Z., Laxer R.M, & Goldbach-Mansky R. (2014). An activating NLRC4 inflammasome mutation causes autoinflammation with recurrent macrophage activation syndrome. Nature genetics, 46(10), 1140-1146.
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2

NLRC4 and Caspase-1 Activation in THP1 Cells

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5×105 transduced THP1 cells were subjected to SDS-PAGE, transferred to nitrocellulose membranes and probed with appropriate antibodies. Antibodies used for protein blots were NLRC4 antibody (1:500 dilution; Millipore, 06-1125), pro-caspase1 antibody (1:1000 dilution; Cell Signaling Technology, 2225), cleaved caspase-1 antibody (1:500 dilution; Cell Signaling Technologies, 4199), HSPA8 antibody (1:2000 dilution; Cell Signaling Technologies, 8444). Horseradish peroxidase (HRP)-conjugated secondary antibodies (Cell Signaling Technologies, 7074) and SuperSignal West Pico chemiluminescent substrate (Pierce) were used to detect primary antibodies. Equal protein loading was assessed by immunoblotting for HSPA8.
Canna S.W., de Jesus A.A., Gouni S., Brooks S.R., Marrero B., Liu Y., DiMattia M.A., Zaal K.J., Montealegre Sanchez G.A., Kim H., Chapelle D., Plass N., Huang Y., Villarino A.V., Biancotto A., Fleisher T.A., Duncan J.A., O’Shea J.J., Benseler S., Grom A., Deng Z., Laxer R.M, & Goldbach-Mansky R. (2014). An activating NLRC4 inflammasome mutation causes autoinflammation with recurrent macrophage activation syndrome. Nature genetics, 46(10), 1140-1146.
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3

Western Blot Analysis of Apoptosis Markers

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PVDF membranes were transferred with 12% SDS‐PAGE and blocked with 5% defatted milk. Primary antibodies were incubated with the protein samples at 4°C overnight followed by incubation of secondary antibody goat anti‐rabbit IgG H&L preadsorbed (Abcam, ab96899, 1/1000) at 37°C for 45 min. MST1 antibody (Abcam, ab245190, 1/1000), cleaved caspase‐1 antibody (Cell Signaling Technology, 89332, 1/1000), NLRP3 antibody (Cell Signaling Technology, 15101, 1/1000), beclin1 (Abclonal, A7353, 1:2000), LC3 (Cell Signaling Technology, 4108, 1:2000), p62 (Abcam, ab91526, 1:1000), and MST1 antibody (Abcam, ab245190, 1/1000) were the primary antibodies used. β‐Actin was used as the control. Chemiluminescence reagents were used to visualize protein bands and ImagePro plus software 6.0 was used to quantify them.
Liu Q., Lai G., Hu Y., Yang F., Zhang C., Le D., Deng F., Xing X., Tang B., Jie H., Liang Y, & Lei E. (2024). CircRbms1 fosters MST1 mRNA and protein levels to motivate myocardial ischaemia–reperfusion injury via autophagic status. ESC Heart Failure, 11(2), 1205-1217.
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