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2 protocols using camphene

1

Antifungal Compounds Screening Protocol

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Miconazole, diflucan (fluconazole), and 5-fluorocytosine were purchased from Combi-Blocks Inc. (San Diego, CA, USA). Itraconazole and voriconazole were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). 2-(4-Thiazolyl) benzimidazole (thiabendazole, TBZ) was obtained from San-ai Oil Co., Ltd. (Tokyo, Japan). Amphotericin B and terpinen-4-ol were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), and α-pinene, camphene, 3-carene, limonene, γ-terpinene, 1,8-cineole, and p-cymene were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).
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2

Monoterpene Induction in Cell Cultures

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Cells were induced with the following chemicals: Camphor (Tokyo Chemical Industry, CAT#: C0011); borneol (Tokyo Chemical Industry, CAT#: B0525); fenchone (Tokyo Chemical Industry, CAT#: F0164); fenchol (Alfa Aesar, CAT#: L03211); eucalyptol (Acros Organics, CAT#: 110340050); verbenol (Sigma Aldrich, CAT#: 247065-5G); camphene (Tokyo Chemical Industry, CAT#: C0009); carvone (Acros Organics, CAT#: 154590050); alpha-pinene (Acros Organics, CAT#: 131270050); limonene (Tokyo Chemical Industry, CAT#: L0132); linalool (Combi-Blocks, CAT#: QH-3254). All monoterpenes were flushed with argon and stored at -20°C after opening. It should be noted that the commercially available camphene used in this study is >78% pure and contains as much as 20% tricyclene.
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