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Human cystatin c duoset catalog dy1196

Manufactured by R&D Systems
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The Human Cystatin C Duoset Catalog# DY1196 is a laboratory reagent kit designed for the quantitative measurement of human cystatin C levels in cell culture supernatants, serum, plasma, urine, and other biological fluids. The kit contains the necessary components for the development of a sandwich enzyme-linked immunosorbent assay (ELISA) to detect and quantify human cystatin C.

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Lab products found in correlation

Fetal bovine serum (fbs), by Merck Group (1 mentions) F6178, by Merck Group (1 mentions)

2 protocols using human cystatin c duoset catalog dy1196

1

Serum Cystatin C Quantification by ELISA

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Cystatin C was measured by ELISA according to the manufacturer's protocol (R&D Systems Human Cystatin C Duoset Catalog# DY1196). We used a 6 point standard curve with a high standard of 3000 pg/ml. Samples were diluted 1∶2000 in PBS with 10% fetal bovine serum (Sigma Aldrich F6178). Recovery of the IFCC certified reference material for serum cystatin C (ERM-DA 471/IFCC) ranged from 104–114%. The intra- and inter- assay CVs were 3% and 10%, respectively. We calculated estimated glomerular filtration rate (eGFR) using the 2012 CKD-EPI Cystatin C equation [54] .
Peters B.A., Hall M.N., Liu X., Neugut Y.D., Pilsner J.R., Levy D., Ilievski V., Slavkovich V., Islam T., Factor-Litvak P., Graziano J.H, & Gamble M.V. (2014). Creatinine, Arsenic Metabolism, and Renal Function in an Arsenic-Exposed Population in Bangladesh. PLoS ONE, 9(12), e113760.
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2

Estimating GFR from Cystatin C

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GFR is typically estimated from plasma creatinine and/or cystatin C using population-based equations; we calculated eGFR from plasma cystatin C using the 2012 CKD-EPI Cystatin C equation (Inker et al., 2012 (link)). Plasma cystatin C was measured by ELISA according to the manufacturer’s protocol (R&D Systems Human Cystatin C Duoset Catalog# DY1196). We used a 6 point standard curve with a high standard of 3000 pg/ml. Samples were diluted 1:2000 in PBS with 10% fetal bovine serum (Sigma Aldrich F6178). Recovery of the IFCC certified reference material for serum cystatin C (ERM-DA 471/IFCC) was 104%. The intra- and inter-assay CVs were 7% and 10%, respectively.
Peters B.A., Hall M.N., Liu X., Slavkovich V., Ilievski V., Alam S., Siddique A.B., Islam T., Graziano J.H, & Gamble M.V. (2015). Renal function is associated with indicators of arsenic methylation capacity in Bangladeshi adults. Environmental research, 143(0 0), 123-130.
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