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Af5888

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AF5888 is a laboratory equipment product offered by R&D Systems. It is designed for general laboratory use, but a detailed description of its core function is not available at this time.

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Lab products found in correlation

P gsk3beta ser9, by Cell Signaling Technology (2 mentions) Murine anti β actin monoclonal antibody, by Merck Group (2 mentions) V5 and pan 14 3 3 antibody, by Abcam (2 mentions) P pi3k p110δ, by Cell Signaling Technology (2 mentions) P fak tyr861, by Abcam (2 mentions) P stat1, by Cell Signaling Technology (1 mentions) C parp, by Cell Signaling Technology (1 mentions) P jak1, by Cell Signaling Technology (1 mentions) Anti p jak2, by Cell Signaling Technology (1 mentions) Stat1, by Cell Signaling Technology (1 mentions) Pd l1, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Cleaved caspase 9, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions)

4 protocols using af5888

1

Immunoblotting Antibodies for Cell Signaling

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The following antibodies were used in this study: anti-human LNK antibody [AF5888, R&D system Inc, a sheep IgG against E.coli derived recombinant human LNK protein (amino acids 427–575)]; murine anti-β-actin monoclonal antibody (Sigma); V5 and pan 14-3-3 antibody (Abcam); antibodies against p-JAK2 (Tyr1007/1008), JAK2, p-p38 (Thr180/Tyr182), p-ERK1/2 (Thr202/Tyr204), p-JNK1/2 (Tyr183), p-PDK1 (Ser243), p-P70S6 (Thr421/Ser424), p-GSK3beta (Ser9), p-AKT (Ser473) and AKT, p-PI3K p110δ (Tyr485) (from either Cell Signaling Technology or Santa Cruz) and p-FAK Tyr861 (Epitomics).
Ding L.W., Sun Q.Y., Lin D.C., Chien W., Hattori N., Dong X.M., Gery S., Garg M., Doan N.B., Said J.W., Xiao J.F., Yang H., Liu L.Z., Meng X., Huang R.Y., Tang K, & Koeffler H.P. (2014). LNK (SH2B3): paradoxical effects in ovarian cancer. Oncogene, 34(11), 1463-1474.
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2

Melanoma Tissue Array Immunohistochemistry

Cited 1 time
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Melanoma tissue array (ME242a, obtained from US Biomax Inc.) was stained with LNK antibody (R&D, AF5888, the specificity and titration of the antibody for IHC experiment was tested with positive and negative controls56 (link)). Staining of tissue arrays of 163 melanoma patient samples was performed at Melanoma Institute Australia, Sydney, Australia.
Ding L.W., Sun Q.Y., Edwards J.J., Fernández L.T., Ran X.B., Zhou S.Q., Scolyer R.A., Wilmott J.S., Thompson J.F., Doan N., Said J.W., Venkatachalam N., Xiao J.F., Loh X.Y., Pein M., Xu L., Mullins D.W., Yang H., Lin D.C, & Koeffler H.P. (2019). LNK suppresses interferon signaling in melanoma. Nature Communications, 10, 2230.
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3

Protein Extraction and Western Blotting Protocols

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Total proteins were extracted using M-PER mammalian Protein Extraction Reagent (Thermo Scientifics). The following antibodies were used in this study: anti-human LNK antibody (AF5888, R&D system Inc, 1:2000); anti-mouse/human Lnk/LNK ((A-12) sc-393709, Santa Cruz Biotechnology Inc, 1:500); anti-β-actin (a1978, Sigma-Aldrich, 1:6000); anti-p-JAK2 (Tyr1007/1008, 3776S, Cell Signaling Technology Inc, 1:2500), JAK2 (3230S, Cell Signaling Technology Inc, 1:2500), p-JAK1 (Tyr1022/1023, 3331, Cell Signaling Technology Inc, 1:2500), JAK1 (3344, Cell Signaling Technology Inc, 1:2500), p-STAT1 (Tyr701, 7649, Cell Signaling Technology Inc, 1:2500), STAT1 (9172, Cell Signaling Technology Inc, 1:2500), IRF1 (8478, Cell Signaling Technology Inc, 1:2500), Cleaved Caspase-3 (9661, Cell Signaling Technology Inc, 1:2000), Cleaved Caspase-9 (9501, Cell Signaling Technology Inc, 1:2000), BCL2 (4223, Cell Signaling Technology Inc, 1:2000), BIM (2933, Cell Signaling Technology Inc, 1:2000), c-PARP (Asp214, 5625, Cell Signaling Technology Inc, 1:2500), PD-L1 (13684, Cell Signaling Technology Inc, 1:2000) and GAPDH (2118, Cell Signaling Technology Inc, 1:6000). Detail of all the antibodies are listed in Supplementary Table 2, the source data of western blots are provided in Supplementary Fig. 12.
Ding L.W., Sun Q.Y., Edwards J.J., Fernández L.T., Ran X.B., Zhou S.Q., Scolyer R.A., Wilmott J.S., Thompson J.F., Doan N., Said J.W., Venkatachalam N., Xiao J.F., Loh X.Y., Pein M., Xu L., Mullins D.W., Yang H., Lin D.C, & Koeffler H.P. (2019). LNK suppresses interferon signaling in melanoma. Nature Communications, 10, 2230.
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4

Immunoblotting Antibodies for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used in this study: anti-human LNK antibody [AF5888, R&D system Inc, a sheep IgG against E.coli derived recombinant human LNK protein (amino acids 427–575)]; murine anti-β-actin monoclonal antibody (Sigma); V5 and pan 14-3-3 antibody (Abcam); antibodies against p-JAK2 (Tyr1007/1008), JAK2, p-p38 (Thr180/Tyr182), p-ERK1/2 (Thr202/Tyr204), p-JNK1/2 (Tyr183), p-PDK1 (Ser243), p-P70S6 (Thr421/Ser424), p-GSK3beta (Ser9), p-AKT (Ser473) and AKT, p-PI3K p110δ (Tyr485) (from either Cell Signaling Technology or Santa Cruz) and p-FAK Tyr861 (Epitomics).
Ding L.W., Sun Q.Y., Lin D.C., Chien W., Hattori N., Dong X.M., Gery S., Garg M., Doan N.B., Said J.W., Xiao J.F., Yang H., Liu L.Z., Meng X., Huang R.Y., Tang K, & Koeffler H.P. (2014). LNK (SH2B3): paradoxical effects in ovarian cancer. Oncogene, 34(11), 1463-1474.
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