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Qubit dsdna assay

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The Qubit dsDNA assay is a fluorescence-based method for quantifying double-stranded DNA (dsDNA) in solution. It provides a sensitive and accurate measurement of dsDNA concentration in a sample.

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Lab products found in correlation

Qiaamp dna ffpe tissue kit, by Qiagen (18 mentions) Qiaamp circulating nucleic acid kit, by Qiagen (5 mentions) Nanodrop, by Thermo Fisher Scientific (4 mentions) Qubit 2.0 fluorometer, by Thermo Fisher Scientific (3 mentions) Nextseq 500 sequencer, by Illumina (2 mentions) Ampure bead, by Beckman Coulter (2 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions) Ercc rna spike in control mix, by Thermo Fisher Scientific (2 mentions) Agilent 5200 fragment analyzer system, by Agilent Technologies (2 mentions) Zymo ribofree total rna library kit, by Zymo Research (2 mentions) High sensitivity dna kit, by Agilent Technologies (2 mentions) Agilent bioanalyzer 2100 system, by Agilent Technologies (2 mentions) Tri reagent, by Zymo Research (2 mentions) Direct zol rna miniprep, by Zymo Research (2 mentions) Hiseq 4000 platform, by Illumina (2 mentions) Agilent 2100, by Agilent Technologies (2 mentions) Quant it picogreen dsdna assay, by Thermo Fisher Scientific (2 mentions) Kapa2g robust hotstart readymix, by Roche (2 mentions) Ampure xp bead, by Beckman Coulter (2 mentions) Agencourt ampure xp kit, by Beckman Coulter (2 mentions)

43 protocols using qubit dsdna assay

1

Genomic DNA and cfDNA Extraction from FFPE and Liquid Samples

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Genomic DNA was purified from FFPE tumor and MPE cell pellet samples using a QIAamp DNA FFPE tissue kit (Qiagen, Hilden, Germany). Cell‐free DNA (cfDNA) was isolated from MPE supernatant and plasma samples using a QIAamp Circulating Nucleic Acid kit, according to the manufacturer's standard protocol (Qiagen).23 Quantification of DNA obtained from tissue, plasma, MPE supernatant, and cell pellet samples was performed using the Qubit dsDNA assay (Life Technologies, Carlsbad, CA, USA).
Guo Z., Xie Z., Shi H., Du W., Peng L., Han W., Duan F., Zhang X., Chen M., Duan J., Lin J., Chen X., Lizaso A.A., Han‐Zhang H., He J, & Yin W. (2019). Malignant pleural effusion supernatant is an alternative liquid biopsy specimen for comprehensive mutational profiling. Thoracic Cancer, 10(4), 823-831.
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2

Extracting High-Quality Genomic DNA

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Genomic DNA was extracted from patient-derived Lymphoblastoid cell lines at Genetic Repositories Australia (GRA, Sydney, Australia), or extracted from blood or saliva samples at the Australian Genomics Research Facility or in the research laboratories of the Neurogenetics Research Group at the University of Adelaide. Commercially available kits were used for DNA extraction in all cases. DNA integrity and quantity were verified by gel electrophoresis and Qubit dsDNA assay (Life Technologies).
van Eyk C.L., Webber D.L., Minoche A.E., Pérez-Jurado L.A., Corbett M.A., Gardner A.E., Berry J.G., Harper K., MacLennan A.H, & Gecz J. (2021). Yield of clinically reportable genetic variants in unselected cerebral palsy by whole genome sequencing. NPJ Genomic Medicine, 6, 74.
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3

FFPE DNA Extraction and Quantification

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Genomic DNA was extracted from FFPE samples using the QIAamp DNA FFPE tissue kit (Qiagen, Carlsbad, CA, USA) according to the manufacturer’s instructions. DNA concentrations were measured using the Qubit dsDNA assay (Life Technologies, Carlsbad, CA, USA).
Xu T., Zhang Y., Zhang J., Qi C., Liu D., Wang Z., Li Y., Ji C., Li J., Lin X., Hou T., Liu H., Zhang L., Han-Zhang H., Shen L, & Wang X. (2020). Germline Profiling and Molecular Characterization of Early Onset Metastatic Colorectal Cancer. Frontiers in Oncology, 10, 568911.
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4

Genomic DNA Extraction from Multiple Sources

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Genomic DNA was either extracted from patient-derived lymphoblastoid cell lines (LCLs) at Genetic Repositories Australia (GRA, Sydney, Australia), extracted from blood samples at the Australian Genomics Research Facility (AGRF), or extracted from buccal samples as previously described.51 (link) DNA integrity and quantity were verified by gel electrophoresis and Qubit dsDNA assay (Life Technologies).
van Eyk C.L., Corbett M.A., Frank M.S., Webber D.L., Newman M., Berry J.G., Harper K., Haines B.P., McMichael G., Woenig J.A., MacLennan A.H, & Gecz J. (2019). Targeted resequencing identifies genes with recurrent variation in cerebral palsy. NPJ Genomic Medicine, 4, 27.
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5

Extracting DNA from FFPE Breast Tissue

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DNA from paraffin-embedded breast cancer tissues was extracted using QIAamp DNA FFPE tissue kit (Qiagen, California, US) according to the manufacturer’s instructions. DNA concentration was measured by Qubit dsDNA assay (Life Technologies, California, US).
Jia M., Liao N., Chen B., Zhang G., Wang Y., Li X., Cao L., Mok H., Ren C., Li K., Li C., Wen L., Lin J., Wei G, & Balch C.M. (2021). PIK3CA somatic alterations in invasive breast cancers: different spectrum from Caucasians to Chinese detected by next generation sequencing. Breast Cancer (Tokyo, Japan), 28(3), 644-652.
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6

Circulating Nucleic Acid Extraction

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We used a QIAamp Circulating Nucleic Acid kit and QIAamp DNA FFPE tissue kit to extract cfDNA and genomic DNA from plasma/BALF and tissue samples, respectively, according to the manufacturer’s standard protocol (Qiagen, Hilden, Germany). DNA was quantified using the Qubit dsDNA assay (Life Technologies, Carlsbad, CA, USA).
Zeng D., Wang C., Mu C., Su M., Mao J., Huang J., Xu J., Shao L., Li B., Li H., Li B., Zhao J, & Jiang J. (2021). Cell-free DNA from bronchoalveolar lavage fluid (BALF): a new liquid biopsy medium for identifying lung cancer. Annals of Translational Medicine, 9(13), 1080.
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7

Genomic DNA Extraction from FFPE Samples

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Genomic DNA were extracted tissue samples using a QIAamp DNA FFPE tissue kit, according to the manufacturer’s standard protocol (Qiagen, Hilden, Germany). DNA was quantified using the Qubit dsDNA assay (Life Technologies, Carlsbad, CA, USA).
Wang Q., Wu L., Yu J., Li G., Zhang P., Wang H., Shao L., Liu J, & Shen W. (2022). Comparison of tumor and two types of paratumoral tissues highlighted epigenetic regulation of transcription during field cancerization in non-small cell lung cancer. BMC Medical Genomics, 15, 66.
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8

Extracting DNA from Tumor Tissue

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Tumor tissue samples obtained by either diagnostic or surgical procedures were used for ROS1 fusion detection. DNA was extracted using QIAamp DNA FFPE tissue kit (Qiagen, Manchester, UK) according to the manufacturer’s instructions. DNA concentration was measured using Qubit dsDNA assay (Life Technologies, Carlsbad, CA, USA).
He Y., Sheng W., Hu W., Lin J., Liu J., Yu B., Mao X., Zhang L., Huang J, & Wang G. (2019). Different Types of ROS1 Fusion Partners Yield Comparable Efficacy to Crizotinib. Oncology Research, 27(8), 901-910.
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9

Isolation and Quantification of cfDNA

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Cell-free DNA (cfDNA) and genomic DNA were isolated from plasma and tissue samples using a QIAamp Circulating Nucleic Acid kit or QIAamp DNA FFPE tissue kit, respectively, according to the manufacturer’s standard protocol (Qiagen, Hilden, Germany). Quantification of DNA obtained from plasma and tissue samples was performed using the Qubit dsDNA assay (Life Technologies, Carlsbad, CA, USA).
Chen B., Zhang G., Li X., Ren C., Wang Y., Li K., Mok H., Cao L., Wen L., Jia M., Li C., Guo L., Wei G., Lin J., Li Y., Zhang Y., Han-Zhang H., Liu J., Lizaso A, & Liao N. (2020). Comparison of BRCA versus non-BRCA germline mutations and associated somatic mutation profiles in patients with unselected breast cancer. Aging (Albany NY), 12(4), 3140-3155.
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10

Metastatic Tissue DNA Extraction

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DNA of metastatic tumor tissue was extracted using a QIAamp DNA FFPE tissue kit (Qiagen, California, USA) according to the manufacturer’s instructions. DNA concentration was measured with a Qubit dsDNA assay (Life Technologies, California, USA).
He Y., Ma X., Chen K., Liu F., Cai S., Han-Zhang H., Hou T., Xiang J, & Peng J. (2020). Perioperative Circulating Tumor DNA in Colorectal Liver Metastases: Concordance with Metastatic Tissue and Predictive Value for Tumor Burden and Prognosis. Cancer Management and Research, 12, 1621-1630.
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