To access the changes in mitochondrial membrane potential, tetramethylrhodamine ethyl ester (TMRE) Mitochondrial Membrane Potential Assay Kit (Abcam, ab113852) was utilized as per manufacture’s protocol. Briefly, Cells were plated in CELLview 4‐compartment glass‐bottom tissue culture dishes (Greiner Bio‐One, 627,870), and treated as per experimental condition. Cells were then incubated for 30 min at room temperature with 50 nM TMRE and and NucBlue™ Live ReadyProbes™ Reagent (Thermo Fisher Scientific, R3760) and proceed for live‐cell imaging with the alpha Plan‐Apochromat 100×/1.46 Oil DIC M27 objective on the Zeiss LSM 880 with Airyscan. Prior to image analysis, raw .czi files were automatically processed into deconvoluted Airyscan images using the Zen software. Leica SP8 LSM, fitted with STED module, was used to perform live‐cell super‐resolution imaging.
Cellview 4 compartment glass bottom tissue culture dishes
Manufactured by Greiner
CELLview 4‐compartment glass‐bottom tissue culture dishes are a laboratory equipment product designed for cell culture applications. The dishes provide a transparent glass bottom for improved microscopy and imaging. They feature four separate compartments to accommodate multiple sample types or experimental conditions simultaneously.
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Lab products found in correlation
2 protocols using cellview 4 compartment glass bottom tissue culture dishes
1
Mitochondrial Membrane Potential Imaging
2
Live-cell Mitochondrial Imaging
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Cells were plated in CELLview 4‐compartment glass‐bottom tissue culture dishes (Greiner Bio‐One, 627870), PS, 35/10 mm. Dyes (100 nM 10‐N‐nonyl acridine orange, 15 nM TMRE, 5 μM Rho123, and/or 200 nM MitoTracker Green; Invitrogen) were added to cell culture media and incubated 1–3 h prior to live‐cell imaging with the alpha Plan‐Apochromat 100×/1.46 Oil DIC M27 objective on the Zeiss LSM 880 with Airyscan. Prior to image analysis, raw .czi files were automatically processed into deconvoluted Airyscan images using the Zen software. HeLa cells imaged with STED were stained with 50 nM TMRM (Invitrogen) for 30 min before imaging. Leica SP8 LSM, fitted with STED module, was used to perform live‐cell super‐resolution imaging.
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