Anti dig hrp igg fraction monoclonal

Antisense single-stranded DNA probe (Supplementary Table S4) was synthesized and end-labeled with digoxigenin (DIG) (Roche). ISH or FISH was performed in formalin-fixed paraffin-embedded HCC sections or slides cultured with cultured HCC cells. The pre-hybridization, hybridization, anti-DIG-HRP IgG fraction monoclonal (Jackson, 200-032-156) incubation (1:200) and stained with DAB (Servicebio, G1211) was performed as described in previous studies [7 (link), 26 (link), 49 (link)]. Stained ISH or FISH sections were imaged with a ZEISS Axio Vert.A1 microscope and at least 10 representative images were collected for statistical analysis. The ISH or FISH staining was performed “blind” with respect to the different treatments.

Antisense single-stranded DNA probe (Supplementary Table S3) was synthesized and end-labeled with digoxigenin (DIG) (Roche). ISH or FISH was performed in formalin-fixed paraffin-embedded melanoma sections or slides covered with cultured melanoma cells. The pre-hybridization, hybridization, anti-DIG-HRP IgG fraction monoclonal (Jackson, 200-032-156) incubation (1:200), and stained with DAB (Servicebio, G1211) was performed as described in previous studies [38 (link), 39 (link)]. Stained ISH or FISH sections were imaged with an Olympus FV1000 Confocal Laser Scanning Microscope or a ZEISS Axio Vert.A1 microscope and at least 10 representative images were collected for statistical analysis. The ISH or FISH staining was performed “blind” with respect to the different treatments.

Antisense single-stranded DNA probe (Additional file 6: Table S5) was synthesized and end-labelled with digoxigenin (DIG) (Roche). ISH or FISH was performed in formalin-fixed paraffin-embedded melanoma sections or slides covered with cultured melanoma cells. The pre-hybridization, hybridization, anti-DIG-HRP IgG fraction monoclonal (Jackson, 200-032-156) incubation (1:200) and stained with DAB (Servicebio, G1211) was performed as described in previous studies. Stained ISH or FISH sections were imaged with a ZEISS Axio Vert.A1 microscope and at least 10 representative images were collected for statistical analysis. The ISH or FISH staining was performed “blind” with respect to the different treatments [44 (link)]. Co-localization of LINP1 with eIF2α in cSCC cells was detected using FISH for LINP1 and immunofluorescence staining for eIF2α and observed by confocal microscope.