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Cy3 ttaggg 3

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Cy3-(TTAGGG)3 is a synthetic oligonucleotide consisting of three repeats of the DNA sequence TTAGGG labeled with the Cy3 fluorescent dye. It is used as a molecular probe for research purposes.

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Lab products found in correlation

Anti 53bp1, by Novus Biologicals (1 mentions) Anti pml, by Santa Cruz Biotechnology (1 mentions) Anti pcna, by GeneTex (1 mentions) Anti smarcal1, by Santa Cruz Biotechnology (1 mentions) Anti slx4, by Novus Biologicals (1 mentions) Anti rad51, by Santa Cruz Biotechnology (1 mentions)

2 protocols using cy3 ttaggg 3

1

Multiparametric Imaging of DNA Damage

Cited 1 time
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IF-FISH was performed as previously described [50 (link), 58 (link)].
Fluorescent probe is Cy3-(TTAGGG)3 (Panagene). Primary antibodies include anti-53BP1 (Novus Biologicals), anti-RPA2 (EMD Millipore), anti-PCNA (Genetex), anti-PML (Santa Cruz), anti-SMARCAL1 (Santa Cruz), anti-SLX4 (Novus Biologicals), anti-pDNA-PKcs (S2056) (Abcam) and anti-Rad51 (Santa Cruz). Secondary antibodies include DyLight488 conjugated anti-rabbit (Multisciences), DyLight488 conjugated anti-mouse (Multisciences).
Zhang T., Zhang Z., Shengzhao G., Li X., Liu H, & Zhao Y. (2019). Strand break-induced replication fork collapse leads to C-circles, C-overhangs and telomeric recombination. PLoS Genetics, 15(2), e1007925.
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2

Telomeric Sister Chromatid Exchange Analysis

Cited 1 time
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The telomeric sister chromatid exchange (T-SCE) was determined by CO-FISH, which is performed as described previously [58 (link), 76 (link)]. Fluorescent probes are Cy3-(TTAGGG)3 (Panagene) and FITC-(CCTAAA)3 (Panagene).
Zhang T., Zhang Z., Shengzhao G., Li X., Liu H, & Zhao Y. (2019). Strand break-induced replication fork collapse leads to C-circles, C-overhangs and telomeric recombination. PLoS Genetics, 15(2), e1007925.
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