Sciclone and zephyr liquid handling robotics

Manufactured by PerkinElmer

The Sciclone and Zephyr liquid handling robotics are automated liquid handling systems designed for various laboratory applications. The Sciclone offers precise and accurate liquid handling capabilities, while the Zephyr provides efficient sample preparation and assay automation. Both systems are intended to streamline and automate liquid handling tasks, improving workflow efficiency and reproducibility in research and diagnostic laboratories.

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Lab products found in correlation

Experion automated electrophoresis system, by Bio-Rad (3 mentions) Truseq stranded mrna lt sample preparation kit, by Illumina (3 mentions) Qubit 2.0 fluorometric quantitation system, by Thermo Fisher Scientific (3 mentions) Hiseq 3000 4000 platform, by Illumina (2 mentions) Rneasy mini kit, by Qiagen (2 mentions) Real time analysis software, by Illumina (1 mentions) Hiseq 3000 4000, by Illumina (1 mentions) Rneasy purification kit, by Qiagen (1 mentions) Truseq stranded mrna sample preparation kit, by Illumina (1 mentions) Hiseq 3000, by Illumina (1 mentions)

4 protocols using sciclone and zephyr liquid handling robotics

RNA Sequencing Library Preparation

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RNA was isolated using the RNeasy Mini kit (Qiagen, 74106). The amount of total RNA was quantified using Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the RNA integrity number (RIN) was determined using Experion Automated Electrophoresis System (Bio-Rad). RNA-seq libraries were prepared with TruSeq Stranded mRNA LT sample preparation kit (Illumina) using Sciclone and Zephyr liquid handling robotics (PerkinElmer). Library amount was quantified using Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using Experion Automated Electrophoresis System (Bio-Rad). Libraries were pooled, diluted and sequenced using the Illumina HiSeq3000/4000 platform with the 50 bp single-read configuration.

Schick S., Rendeiro A.F., Runggatscher K., Ringler A., Boidol B., Hinkel M., Májek P., Vulliard L., Penz T., Parapatics K., Schmidl C., Menche J., Boehmelt G., Petronczki M., Mueller A.C., Bock C, & Kubicek S. (2019). Systematic characterization of BAF mutations provides insights into intra-complex synthetic lethalities in human cancers. Nature genetics, 51(9), 1399-1410.

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RNA Sequencing Library Preparation

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RNA-seq library preparation and sequencing

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The amount of total RNA was quantified using Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the RNA integrity number (RIN) was determined using Experion Automated Electrophoresis System (Bio-Rad). RNA-seq libraries were prepared with TruSeq Stranded mRNA LT sample preparation kit (Illumina) using Sciclone and Zephyr liquid handling robotics (PerkinElmer). Library amount was quantified using Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using Experion Automated Electrophoresis System (Bio-Rad). For sequencing 6 libraries were pooled, diluted and sequenced on Illumina HiSeq 3000/4000 using 75 bp paired-end chemistry. Base calls provided by the Illumina Realtime Analysis software were converted into BAM format using Illumina2bam and demultiplexed using BamIndexDecoder (https://github.com/wtsi-npg/illumina2bam).

Kosack L., Wingelhofer B., Popa A., Orlova A., Agerer B., Vilagos B., Majek P., Parapatics K., Lercher A., Ringler A., Klughammer J., Smyth M., Khamina K., Baazim H., de Araujo E.D., Rosa D.A., Park J., Tin G., Ahmar S., Gunning P.T., Bock C., Siddle H.V., Woods G.M., Kubicek S., Murchison E.P., Bennett K.L., Moriggl R, & Bergthaler A. (2019). The ERBB-STAT3 Axis Drives Tasmanian Devil Facial Tumor Disease. Cancer Cell, 35(1), 125-139.e9.

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RNA Sequencing Using RNeasy and TruSeq

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RNA was isolated using the RNeasy purification kit (Quiagen). RNA-seq libraries were prepared with TruSeq stranded mRNA sample preparation kit (Illumina) using Sciclone and Zephyr liquid handling robotics (Perki-nElmer). The libraries were sequenced on HiSeq 3000 (Illumina) platform using 50 bp single-read chemistry.

Brandstetter B., Dalwigk K., Platzer A., Niederreiter B., Kartnig F., Fischer A., Vladimer G.I., Byrne R.A., Sevelda F., Holinka J., Pap T., Steiner G., Superti-Furga G., Smolen J.S., Kiener H.P, & Karonitsch T. (2019). FOXO3 is involved in the tumor necrosis factor-driven inflammatory response in fibroblast-like synoviocytes. Laboratory investigation; a journal of technical methods and pathology, 99(5).

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