1281 adiabatic bomb calorimeter

Samples of dietary ingredients and the two diets were analysed for CP by Kjeldahl-N x 6.25 [16 ], fat using ASE 350 Accelerated Solvent Extractor, dry matter (DM) by drying to constant weight at 104°C [16 ], ash by incineration at 550°C [16 ], acid detergent fiber (ADF) and NDF using a fiber analyzer system (Ankom200, ANKOM Technologies, Fairport, NY, USA) with filter bags (Ankom F58, ANKOM Technologies). Gross energy (GE) content was determined by a Parr 1281 Adiabatic Bomb Calorimeter (Parr Instruments, Moline, IL, USA) according to [17 ]. Phosphorus and calcium content of the diets were analysed by atomic absorption spectrophotometry according to methods described by [18 ]. Analysis of amino acids in the diets was carried out according to [16 ] using Biochrom 30 Amino Acid Analyzer. Tryptophan in the diets was determined according to [16 ] using high-performance liquid chromatography system (Dionex UltiMate 3000, Dionex Softron GmbH, Germering, Germany) equipped with fluorescent detector (Shimadzu RF- 535, Shimadzu Corp, Kyoto, Japan). Glucosinolate level in the experimental RSM-diet was determined by Eurofins Food & Feed Testing Norway AS (Kambo, Moss, Norway) using liquid chromatography coupled with diode array detector- method (LC-DAD).

Samples of the diets were analysed for crude protein (CP) by Kjeldahl-N × 6.25 (EC No 152/2009), crude fat using ASE® 350 Accelerated Solvent Extractor, dry matter (DM) by drying to constant weight at 104 °C (EC No 152/2009), ash by incineration at 550 °C (EC No 152/2009), acid detergent fiber (ADF) and neutral detergent fibre (NDF) using a fibre analyser system (Ankom200; ANKOM Technologies, Fairport, NY, USA) with filter bags (Ankom F58; ANKOM Technologies). Gross energy (GE) content was determined by a Parr 1281 Adiabatic Bomb Calorimeter (Parr Instruments, Moline, IL, USA) according to ISO (1998). Analysis of amino acids in the diets were carried out according to EC (2009) using Biochrom 30 Amino Acid Analyzer. Tryptophan in the diets was determined according to EC (2009) using high-performance liquid chromatography system (Dionex UltiMate 3000, Dionex Softron GmbH, Germering, Germany) and the fluorescent detector (Shimadzu RF-535; Shimadzu Corp., Kyoto, Japan).

The feed was dried and ground prior to analysis, and analyses were performed in duplicate for dry matter by drying to a constant weight at 104 °C, for ash by combustion at 550 °C, for crude protein by Kjeldahl nitrogen × 6.25 according to Commission Regulation (EC) No 152/2009, and for starch as described in McCleary et al. [25 (link)]. Lipid was determined after extraction with petroleum ether and acetone (70/30) on an accelerated solvent extractor (ASE 200) (Dionex Corp, Sunnyvale, CA, USA), while gross energy was established with a PARR 1281 Adiabatic bomb calorimeter (Parr Instruments, Moline, IL, USA) according to ISO 9831. Amino acids were analysed according to Commission Regulation (EC) No 152/2009, for all amino acids except tryptophan, on a Biochrom 30 amino acid analyser (Biochrom Ltd,. Cambridge, UK). Tryptophan was analysed according to Commission Regulation (EC) No 152/2009 with a Dionex Ultimate 3000 HPLC system (Dionex Softron GmbH, Germering, Germany) and a Shimadzu RF-535 fluorescence detector (Shimadzu Corporation, Kyoto, Japan).