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Human non targeting sirna

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Human non-targeting siRNA is a synthetic, double-stranded RNA molecule that is designed to not target any known human gene sequences. It can be used as a control in RNA interference (RNAi) experiments to monitor non-specific effects.

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Lab products found in correlation

Interleukin 4 (il 4), by Thermo Fisher Scientific (3 mentions) Neon transfection system 100 ml kit, by Thermo Fisher Scientific (2 mentions) Alox15 trilencer 27 human sirna, by OriGene (2 mentions) Resuspension buffer t, by Thermo Fisher Scientific (2 mentions) Neon transfection system 100 l kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Non-targeting siRNA SiRNA targeting SiRNAs

3 protocols using human non targeting sirna

1

siRNA Transfection of Macrophages

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Cells (1.5 3 106) were subjected to electroporation using Neonâ Transfection System 100 mL Kit (Thermo Fisher Scientific). To maximize the efficiency, the Neon 24 optimization protocol was applied according to the manufacturer’s instruction which varied in pulse voltage, pulse width and the number of pulses. Macrophages, differentiated with M-CSF for 6 days, were harvested using PBS plus 1 mM EDTA and the cell pellet was resuspended in 1 mL of PBS. 20 mM of human non-targeting siRNA (Thermo Fisher Scientific; D-001810-01-05) and 20 mM of ALOX15 Trilencer-27 Human siRNA (OriGene, SR300171) was resuspended in the Resuspension Buffer T (Thermo Fisher Scientific) and added to the cells before electroporation. The electroporated cells were transferred immediately to a 12-well containing 1 mL of the corresponding growth medium plus 20 ng/ml IL-4 (Peprotech) and then incubated for another 48 h in a 5% CO2 incubator.
Jordan P.M., Gerstmeier J., Pace S., Bilancia R., Rao Z., Börner F., Miek L., Gutiérrez-Gutiérrez Ó., Arakandy V., Rossi A., Ialenti A., González-Estévez C., Löffler B., Tuchscherr L., Serhan C.N, & Werz O. (2020). Staphylococcus aureus-Derived α-Hemolysin Evokes Generation of Specialized Pro-resolving Mediators Promoting Inflammation Resolution. Cell reports, 33(2), 108247.
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2

Monocyte-Derived Macrophage Silencing

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Freshly isolated monocytes were differentiated with M‐CSF (20 ng mL−1) to MDMM‐CSF and then subjected to electroporation using Neon Transfection System 100 µL Kit (Thermo Fisher Scientific) as described by Jordan et al.[15] In brief, MDMM‐CSF (1.5 × 106) were resuspended in 1 mL of PBS and 20 µm of human non‐targeting siRNA (Thermo Fisher Scientific; D‐001810‐01‐05) or 20 µm of ALOX15A Trilencer‐27 Human siRNA (OriGene, SR300171) were added to the cells prior to electroporation. The electroporated cells were resuspended in 1 mL of the corresponding growth medium in the presence of 20 ng mL−1 IL‐4 (Peprotech) and incubated for 48 h at 37 °C and 5% CO2.
Börner F., Pace S., Jordan P.M., Gerstmeier J., Gomez M., Rossi A., Gilbert N.C., Newcomer M.E, & Werz O. (2022). Allosteric Activation of 15‐Lipoxygenase‐1 by Boswellic Acid Induces the Lipid Mediator Class Switch to Promote Resolution of Inflammation. Advanced Science, 10(6), 2205604.
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3

siRNA Transfection of Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells (1.5 3 106) were subjected to electroporation using Neonâ Transfection System 100 mL Kit (Thermo Fisher Scientific). To maximize the efficiency, the Neon 24 optimization protocol was applied according to the manufacturer’s instruction which varied in pulse voltage, pulse width and the number of pulses. Macrophages, differentiated with M-CSF for 6 days, were harvested using PBS plus 1 mM EDTA and the cell pellet was resuspended in 1 mL of PBS. 20 mM of human non-targeting siRNA (Thermo Fisher Scientific; D-001810-01-05) and 20 mM of ALOX15 Trilencer-27 Human siRNA (OriGene, SR300171) was resuspended in the Resuspension Buffer T (Thermo Fisher Scientific) and added to the cells before electroporation. The electroporated cells were transferred immediately to a 12-well containing 1 mL of the corresponding growth medium plus 20 ng/ml IL-4 (Peprotech) and then incubated for another 48 h in a 5% CO2 incubator.
Jordan P.M., Gerstmeier J., Pace S., Bilancia R., Rao Z., Börner F., Miek L., Gutiérrez-Gutiérrez Ó., Arakandy V., Rossi A., Ialenti A., González-Estévez C., Löffler B., Tuchscherr L., Serhan C.N, & Werz O. (2020). Staphylococcus aureus-Derived α-Hemolysin Evokes Generation of Specialized Pro-resolving Mediators Promoting Inflammation Resolution. Cell reports, 33(2), 108247.
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+ Expand

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