Flag-Cry2 and Myc-His-Cry2 were kindly provided from Dr. YingHui Fu (University of California, San Francisco, USA). Myc-HDAC6 was provided from Dr. Jiadong Wang (Peking University Health Science Center, Beijing). HA-p300/PCAF/GCN5/TIP60, HA-Ub, NF-κB-luc, EGFP-p65, Flag-p50, and sh-p65 used in our previous studies [24 (link), 39 (link)]. Anti-Cry2 Ab (Proteintech, 13997-1-AP, Chicago, USA; Abcam, ab 155255, Cambridge, UK),anti-p300 Ab (Beyotime,AF6795, Shanghai,China), anti-HDAC6 Ab (Beyotime, AF7071, Shanghai,China), anti-HA Ab (GeneTex, GTX115044, San Antonio, TX, USA), anti-Flag Ab (Sigma-Aldrich, F7425, Saint Louis, Mo, USA), anti-Myc Ab (Origene, TA150121, Rockville, MD, USA), anti-acetylated-lysine Ab (Cell Signaling Technology, #9441, Boston, MA, USA), anti-β-actin Ab (Santa Cruz Biotechnology, sc-9104, CA, USA), anti-GAPDH Ab (GeneTex, GTX627408, CA, USA).
Anti gapdh ab
Manufactured by GeneTex
Sourced in United States
Anti-GAPDH Ab is a laboratory reagent used to detect and quantify the GAPDH protein in biological samples. GAPDH is a commonly used reference protein for Western blot and other protein analysis techniques.
Automatically generated - may contain errors
Lab products found in correlation
Anti flag ab, by Merck Group (1 mentions)
Anti β actin ab, by Santa Cruz Biotechnology (1 mentions)
Folic acid, by Fujifilm (1 mentions)
Methotrexate, by Fujifilm (1 mentions)
Rho kinase inhibitor y 27632, by Merck Group (1 mentions)
Pkh26, by Merck Group (1 mentions)
To pro 3, by Thermo Fisher Scientific (1 mentions)
Infliximab, by Mitsubishi (1 mentions)
Protease and phosphatase inhibitor, by Solarbio (1 mentions)
Chemidoc mp, by Bio-Rad (1 mentions)
Anti akt ab, by Santa Cruz Biotechnology (1 mentions)
Anti erk ab, by Cell Signaling Technology (1 mentions)
Anti p38 ab, by Cell Signaling Technology (1 mentions)
Biospectrum 800 imaging system, by Analytik Jena (1 mentions)
Anti lc3a b, by Abcam (1 mentions)
Anti jnk ab, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
4 protocols using anti gapdh ab
1
Cry2 and Histone Modification Interactions
2
Evaluating Biologics and Small Molecules
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Infliximab (IFX), Etanercept (ETN), Certolizumab Pegol (CZP) were provided by Mitsubishi Tanabe Pharma Corporation (Osaka, Japan), Takeda Pharmaceutical Company Limited (Osaka, Japan) and UCB Japan (Tokyo, Japan), respectively. Methotrexate and folic acid were obtained from Wako Chemicals (Osaka, Japan). Rho kinase inhibitor, Y27632, and PKH-26 were supplied from Sigma (St. Louis, MO, United States). TO-PRO-3 was from Thermo Fisher Scientific (Waltham, MA, United States). Rabbit anti-phospho JNK monoclonal antibody (mAb) and anti-JNK polyclonal antibody (Ab) were purchased from cell signaling technology (Danvers, MA, United States). Anti-GAPDH Ab was from Gene Tex (Irvine, CA, United States).
3
Quantifying Nrf-2 and HO-1 Expressions
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Total protein was extracted from lung tissues using cold RIPA buffer containing protease and phosphatase inhibitors (Solarbio, Beijing, China). The protein expressions of nuclear factor erythroid 2-related factor 2 (Nrf-2) and heme oxygenase-1 (HO-1) in lung tissues were measured using Western blot. Anti-Nrf-2 Ab (1 : 1000, LiankeBio, Hangzhou, China), anti-HO-1 Ab (1 : 500, GeneTex, USA), and anti-GAPDH Ab (1 : 5000, GeneTex, USA) were used. ChemiDoc™ MP (Bio-Rad, Hercules, CA, USA) imaging system was used to record signals. ImageJ was used to quantify the intensity of the protein band, which was normalized to GAPDH in the analysis.
4
Aloperine Modulation of Autophagy Signaling
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Cells were cultured in 10-cm cell culture dishes and treated with aloperine. DMSO was used as a negative control. The whole cellular extract was subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and the separated proteins were electrically transferred to a PVDF membrane (Millipore Corporation, USA). The membrane was blocked with primary antibodies (anti-LC3 Ab; Abcam, USA; anti-GAPDH Ab; GeneTex, USA; anti-AMPK α-1 Ab; Cell signaling, USA; anti-phosphorylated-AMPK α-1 (Thr 172) Ab; Cell Signaling, USA; anti-Akt Ab; Santa Cruz, USA; anti-phosphorylated-Akt (Ser 473) Ab; Santa Cruz, USA; anti-mTOR Ab; Cell Signaling, USA; anti-phosphorylated-mTOR (Ser 2448) Ab; Cell Signaling, USA; anti-p70S6K Ab; Cell Signaling, USA; anti-phosphorylated-p70S6K (Thr 389) Ab; Cell Signaling; anti-p62/SQSTM1 Ab; Abgent, USA; anti-Erk Ab; Cell Signaling, USA; anti-phosphorylated-Erk (Thr 202/204) Ab; Cell Signaling, USA; anti-JNK Ab; Cell Signaling, USA; anti-phosphorylated-JNK (Thr 183/Tyr 185) Ab; Cell Signaling, USA; anti-p38 Ab; Cell Signaling, USA; and anti-phosphorylated (Thr 180/Tyr 182) Ab; Cell Signaling Ab, USA) and was analyzed using the BioSpectrum 800 Imaging System (UVP, CA, USA).
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