Different samples of highland barley flour (300 mg) were placed in 20 mL headspace bottles. Ten microliters of 2-octanol was added as an internal standard. The volatile flavor compounds were analyzed using a 7890B gas chromatograph, 5977B mass spectrometer, and DB-Wax chromatographic column (30 m × 250 μm × 0.25 μm; Agilent Technologies Inc., Santa Clara, CA, USA). The GC conditions were: extraction temperature, 60 °C; preheating time, 15 min; extraction time, 30 min; resolution time, 4 min; splitless mode; and carrier gas, helium. The column flow rate was 1 mL/min, the sample inlet temperature was 250 °C, and the quadrupole temperature was 150 °C. The temperature rise program of the column box was 40 °C for 4 min, followed by 5 °C/min at 245 °C for 5 min. The mass spectrometry conditions included: ionization voltage, −70 eV; ion source temperature, 230 °C; transmission line temperature, 250 °C; mass scan range, m/z 20–400; and solvent delay, 0 min.
Db wax chromatographic column
Manufactured by Agilent Technologies
Sourced in United States
The DB-Wax chromatographic column is a stationary phase used in gas chromatography. It is a high-polarity, polyethylene glycol-based column designed for the separation of polar compounds.
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2 protocols using db wax chromatographic column
1
Volatile Flavor Analysis of Highland Barley Flour
2
Quantification of EC by GC-MS
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EC (Dr. Ehrenstorfer, Germany; purity ≥99.4%) was diluted in musalais and its the concentration was determined by gas chromatography-mass spectrometry using a GCMS-QP2010 Plus apparatus (Shimadzu, Japan). Briefly, 1 μL of sample was injected into a DB-WAX chromatographic column (30 mm × 0.25 mm, 0.25 μm, Agilent, USA). The initial column temperature was 100 °C and increased to 120 °C at a rate of 50 °C/min, then held at 120 °C for 5 min and increased to 200 °C at a rate of 50 °C/min and maintained for 1.5 min. High-purity helium gas (>99.999%) with a flow rate of 1.0 mL/min was used as the carrier gas. Mass spectrometry was performed in selected-ion monitoring mode with a transfer line at 280 °C, electron voltage of 70 eV, and source temperature of 230 °C.
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