Tm3000 tabletop microscope

Three weeks of embryogenic and non-embryogenic calli were selected and were soaked in FAA (Formalin acetic acid) solution for 24 h. The fixed calli were then dehydrated using a graded ethanol series (70%, 80%, 90% and 100% per hour) and then dried using critical point drying (CPD: Leica). The calli were coated with gold and observed using the scanning electron microscope (Hitachi Tabletop MicroscopeTM3000) at 15 kv. Histology analysis was conducted as described by Vega et al. [17 (link)]. The calli were fixed in FAA solution for 24 h and followed by dehydration through a series of ethanol (70%, 80%, 90%, and 100%). The calli were soaked in paraffin wax and then embedded using Tissue Embedding System 2900 (TEC HistoLine Laboratories) in the horizontal orientation using paraffin as embedding medium. The specimen was sectioned at 5 µm and stained using hematoxylin and eosin solution (H&E staining).

Twenty flowers at anthesis stage were sampled from 25 plants of each BC₃ TOMJPE8986 and WT line. All organs but the anthers were carefully removed. Anther cones were then gently opened before observation under a Hitachi Tabletop Microscope TM3000 (Hitachi, Tokyo, Japan). Each sample was captured in three different magnification (40, 80, and 120×). Anther dehiscence was evaluated based on pollen sac opening size and pollen grain dispersion in each sample observed at 80× magnification.

The morphology of Fe-covered copper foams was investigated using a scanning electron microscope (SEM, Hitachi’s Tabletop Microscope TM-3000, Tokyo, Japan), equipped with an EDX module.

Lyophilized sample was first deposited on aluminum stubs and then coated with gold using an ion sputter and then visualized by scanning electron microscope (SEM) from Hitachi Tabletop Microscope (TM3000, Tokyo, Japan).