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Alpha melanocyte stimulating hormone α msh

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Alpha-melanocyte stimulating hormone (α-MSH) is a peptide hormone produced by the pituitary gland. It is involved in the regulation of pigmentation in the skin.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (3 mentions) Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Potassium hydrogen phosphate, by Merck Group (2 mentions) L 3 4 dihydroxyphenylalanine l dopa, by Merck Group (2 mentions) Potassium dihydrogen phosphate, by Merck Group (2 mentions) Phosphate buffered saline (pbs), by Merck Group (2 mentions) Anti phospho pi3k, by Cell Signaling Technology (1 mentions) Anti phospho mtor, by Cell Signaling Technology (1 mentions) Temozolomide, by Merck Group (1 mentions) Anti mmp2, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti mtor, by Cell Signaling Technology (1 mentions) Anti phospho akt, by Cell Signaling Technology (1 mentions) Anti mitf, by Cell Signaling Technology (1 mentions) Anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Anti pi3k, by Cell Signaling Technology (1 mentions) Anti erk1 2, by Cell Signaling Technology (1 mentions) Anti mmp9, by Cell Signaling Technology (1 mentions)

8 protocols using alpha melanocyte stimulating hormone α msh

1

Signaling Pathways in Melanoma and Cell Death

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Melittin, temozolomide, MG132 and alpha-melanocyte stimulating hormone (α-MSH) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Bee venom, Matrigel and Transwell chamber system were obtained from Chung Jin Biotech (Ansan, Korea), BD Biosciences (San Jose, CA, USA) and Corning Costar (Acton, MA, USA), respectively. Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Invitrogen (Grand Island, NY, USA). Anti-phospho-PI3K, anti-PI3K, anti-phospho-AKT, anti-AKT, anti-phospho-mTOR, anti-mTOR, anti-phospho-ERK1/2, anti-ERK1/2, anti-phospho-p38, anti-p38, anti-cleaved caspase-3, anti-cleaved capase-9, anti-MITF, anti-MMP-2, anti-MMP-9 and anti-β-actin antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).
Lim H.N., Baek S.B, & Jung H.J. (2019). Bee Venom and Its Peptide Component Melittin Suppress Growth and Migration of Melanoma Cells via Inhibition of PI3K/AKT/mTOR and MAPK Pathways. Molecules, 24(5), 929.
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2

Melanogenesis Inhibition Assay

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The chemicals 4-(1,1,3,3-tetramethylbutyl)phenylpolyethylene glycol (TritonTM X-100), l-4-hydroxyphenylalanine (l-tyrosine), dimethyl sulfoxide (DMSO), kojic acid, 3-isobutyl-1-methylxanthine (IBMX), potassium hydrogen phosphate, mushroom tyrosinase, potassium dihydrogen phosphate, alpha-melanocyte-stimulating hormone (α-MSH), l-3,4-dihydroxyphenylalanine (l-dopa), and phenylmethylsulfonyl fluoride (PMSF) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Yoon D., Kang M.K., Jung H.J., Ullah S., Lee J., Jeong Y., Noh S.G., Kang D., Park Y., Chun P., Chung H.Y, & Moon H.R. (2023). Design, Synthesis, In Vitro, and In Silico Insights of 5-(Substituted benzylidene)-2-phenylthiazol-4(5H)-one Derivatives: A Novel Class of Anti-Melanogenic Compounds. Molecules, 28(8), 3293.
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3

Melanogenesis Regulation by Natural Compounds

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Sephadex LH-20 column of 25–10 μm was purchased from Pharmacia (Stockholm, Sweden), MCI-gel CHP 20P column of 75–150 μm was purchased from Mitsubishi (Tokyo, Japan), and YMC-gel ODS-A column of 230–70 and 500–400 mesh were purchased from YMC Co (PA, USA). Dimethylsulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), L-DOPA, alpha-melanocyte stimulating hormone (α-MSH), and phosphate-buffered saline (PBS) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were obtained from Invitrogen–Gibco (Grand Island, NY, USA). Antibodies against MITF, tyrosinase, TRP-1, TRP-2, phospho-p38 MAPK, p38 MAPK, phospho-ERK, ERK, phospho-JNK, JNK, phospho-CREB, and CREB purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-mouse and anti-goat IgG antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). The cAMP assay kit was purchased from Cayman Chemical (Ann Arbor, MI). All other reagents were purchased from Sigma–Aldrich Chemical Co. (St. Louis, MO, USA).
Jung H.G., Kim H.H., Paul S., Jang J.Y., Cho Y.H., Kim H.J., Yu J.M., Lee E.S., An B.J., Kang S.C, & Bang B.H. (2015). Quercetin-3-O-β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranoside suppresses melanin synthesis by augmenting p38 MAPK and CREB signaling pathways and subsequent cAMP down-regulation in murine melanoma cells. Saudi Journal of Biological Sciences, 22(6), 706-713.
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4

Melanogenic Inhibitor Evaluation Protocol

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BPA1–BPA13 were synthesized in our laboratory. Mushroom tyrosinase, 3-(4-hydroxyphenyl)-L-alanine (L-tyrosine), L-DOPA, 5-hydroxy-2-hydroxymethyl-4H-4-pyranone (kojic acid), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), alpha-melanocyte-stimulating hormone (α-MSH), phenylmethylsulfonyl fluoride, 4-(1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol (Triton™ X-100), dimethyl sulfoxide (DMSO), ethyl alcohol, potassium dihydrogen phosphate, potassium hydrogen phosphate, benzoylacetonitrile, piperidine, sodium acetate, and several benzaldehydes were purchased from Sigma-Aldrich (St Louis, MO, USA). Dulbecco’s Modified Eagle’s Medium, fetal bovine serum, trypsin, phosphate-buffered saline, penicillin, and streptomycin were obtained from Gibco Life Technologies (Carlsbad, CA, USA). All chemicals and solvents were of analytical grade. The B16F10 mouse melanoma cell line was kindly provided by Professor Hae Young Chung (College of Pharmacy, Pusan National University, Republic of Korea).
Yun H.Y., Kim D.H., Son S., Ullah S., Kim S.J., Kim Y.J., Yoo J.W., Jung Y., Chun P, & Moon H.R. (2015). Design, synthesis, and anti-melanogenic effects of (E)-2-benzoyl-3-(substituted phenyl)acrylonitriles. Drug Design, Development and Therapy, 9, 4259-4268.
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5

Melanogenesis Regulation via Signaling Pathways

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Dimethylsulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), L-DOPA, alpha-melanocyte stimulating hormone (α-MSH) and phosphate buffered saline (PBS) were purchased from Sigma–Aldrich Chemical Co. (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were obtained from Invitrogen–Gibco (Grand Island, NY, USA). The extracellular signal-regulated kinase (ERK1/2), phosphorylated ERK1/2 (p-ERK1/2), c-Jun N-terminal kinase (JNK), phosphorylated JNK (p-JNK), p38, phosphorylated p38 (p-p38), cAMP response element-binding protein (CREB), phosphorylated CREB (p-CREB), microphthalamia-associated transcription factor (MITF), tyrosinase-related protein-1 (Trp-2), tyrosinase-related protein-1 (Trp-1), tyrosinase (TYR), anti-mouse and anti-rabbit IgG antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). All other reagents, including α-MSH were purchased from Sigma–Aldrich Chemical Co.
Li X., Yang H.W., Jiang Y., Oh J.Y., Jeon Y.J, & Ryu B. (2020). Ishophloroglucin A Isolated from Ishige okamurae Suppresses Melanogenesis Induced by α-MSH: In Vitro and In Vivo. Marine Drugs, 18(9), 470.
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6

Cytokine and Hormone Modulation of Melanoma

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Mouse recombinant interferon-gamma (IFNG) was purchased from Cell Signaling Technology (Cat. No. 5222) or BioLegend (Cat. No. 575304). Human recombinant IFNG was purchased from Cell Signaling Technology (Cat. No. 8901) or BioLegend (Cat. No. 575304). Mouse interferon-alpha 2 (IFNA2) recombinant protein was purchased from Affymetrix eBioscience (Cat. No. 14–8312). The typical cytokine specific activity was 1×107 IU/mg. All cytokine treatments were performed at a media concentration of 10 ng/ml (equivalent to 100 IU/ml), as used by others previously (Natarajan et al., 2014 (link); Son et al., 2014 (link)). Alpha melanocyte-stimulating hormone (αMSH) was purchased from Sigma-Aldrich (Cat. No. 581–05-5). The concentration used for mouse and human melanoma/melanocyte cells were 10 nM and 100 nM, respectively. The culture media and cytokines/hormone were refreshed every other day.
Mo X., Raza Kazmi H., Preston-Alp S., Zhou B, & Raza Zaidi M. (2022). Interferon-gamma Induces Melanogenesis Via Post-Translational Regulation of Tyrosinase. Pigment cell & melanoma research, 35(3), 342-355.
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7

Morifolium Extraction and Melanogenesis

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The SP was obtained from Fuji Oil (Osaka, Japan). The CP was obtained from Haishi (Zhoushan, China). The air-dried and powdered aerial parts of C. morifolium were extracted with water. Nuclear fast red, tyrosinase, l-tyrosine, alpha-melanocyte stimulating hormone (α-MSH), and all other chemicals were obtained from the Sigma-Aldrich company (Shanghai, China) and were of analytical grade.
Gui M., Du J., Guo J., Xiao B., Yang W, & Li M. (2014). Aqueous Extract of Chrysanthemum morifolium (菊花 Jú Huā) Enhances the Antimelanogenic and Antioxidative Activities of the Mixture of Soy Peptide and Collagen Peptide. Journal of Traditional and Complementary Medicine, 4(3), 171-176.
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8

Effect of α-MSH and L-DOPA on Melanogenesis

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The alpha-melanocyte-stimulating hormone (α-MSH) and L-3,4-dihydroxyphenylalanine (L-DOPA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies for tyrosinase, TRP-1, and TRP-2 and secondary antibodies were obtained from Santa Cruz biotechnology (Santa Cruz, CA, USA), and antibodies for phospho-p38, phospho-JNK, phospho-ERK, MITF, and actin were obtained from Cell Signaling Technology (Beverly, MA, USA). In addition, C. ovata was obtained from Hanbang Herbal Drug Co. (Jecheon, Korea). All other reagents were of the highest commercially available grade.
Kim Y.S., Lee E.B., Yu Y.J., Kim G.W., Kim W.J, & Choi D.K. (2023). Ethyl Acetate Fraction from a Catalpa ovata G. Don Extract Inhibits ɑ-MSH-Induced Melanogenesis through the cAMP/CREB Pathway. International Journal of Molecular Sciences, 25(1), 151.
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