Peptide synthesis reagents

The Fmoc/tBu protected amino acid building blocks, preloaded 2 chlorotrityl resin, and peptide synthesis reagents were purchased from Chem-impex International Inc, (Wood Dale, IL, USA). Solvents and other reagents were purchased from Millipore Sigma (Milwaukee, WI, USA). All the cell culture supplies were purchased from Corning (Christiansburg, VA, USA) and Fisher Scientific (Waltham, MA, USA). The human triple-negative breast cancer cell line (MDA-MB-231, ATCC No. HTB-26), and normal kidney cell line (HEK-293, ATCC No. CRL-1573) were purchased from ATCC (Manassas, VA, USA). CellTiter 96^® AQueous One Solution cell proliferation assay (MTS) was purchased from Promega (Madison, WI, USA). Cell culture was carried out at 37 °C with 5% CO₂ in a Forma incubator using a T-75 flask.

All N-Fmoc- and orthogonally sidechain-protected amino acids, including Fmoc-S-acetamidomethyl-L-homocysteine, and peptide synthesis reagents were acquired from Chem-Impex Inc. (Wood Dale, IL, USA) and ChemPep. Fisher Scientific (Wellington, FL, USA) provided all of the solvents used, and Sigma-Aldrich (St. Louis, MO, USA) provided the riisopropylsilane (TIPS). Alfa Aesar (Ward Hill, MA, USA) provided the diisopropylethylamine (DIEA) along with the metal salts used in the experiment described. Trifluoroacetic acid (TFA) was purchased from Acros Organics (Fair Lawn, NJ, USA). The Rink Amide resin was bought from Gyros Protein Technologies (Uppsala, Sweden). Acridine orange nonyl bromide was purchased from Thermo Fisher (Eugene, OR, USA). Dialysis cassettes (2000 Da MW cutoff) were purchased from Thermo Fisher.

All N-Fmoc- and orthogonally sidechain-protected amino acids, including Fmoc-S-acetamidomethyl-L-homocysteine, and peptide synthesis reagents were acquired from Chem-Impex Inc. (Wood Dale, IL, USA) and ChemPep. Fisher Scientific (Wellington, FL, USA) provided all of the solvents used, and Sigma-Aldrich (St. Louis, MO, USA) provided the riisopropylsilane (TIPS). Alfa Aesar (Ward Hill, MA, USA) provided the diisopropylethylamine (DIEA) along with the metal salts used in the experiment described. Trifluoroacetic acid (TFA) was purchased from Acros Organics (Fair Lawn, NJ, USA). The Rink Amide resin was bought from Gyros Protein Technologies (Uppsala, Sweden). Acridine orange nonyl bromide was purchased from Thermo Fisher (Eugene, OR, USA). Dialysis cassettes (2000 Da MW cutoff) were purchased from Thermo Fisher.