M csf

Manufactured by Genentech

M-CSF is a purified recombinant human Macrophage Colony-Stimulating Factor (M-CSF) protein. M-CSF is a hematopoietic growth factor that stimulates the survival, proliferation, and differentiation of monocytes and macrophages.

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Lab products found in correlation

Tumor necrosis factor (tnf), by Genentech (1 mentions) Lipopolysaccharides (lps), by InvivoGen (1 mentions) 5z 7 oxozeanol, by Bio-Techne (1 mentions) Rifn α4, by PBL Assay Science (1 mentions) Macs lineage depletion kit, by Miltenyi Biotec (1 mentions) Lipofectamine ltx with plus reagent, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Recombinant murine M-CSF

2 protocols using m csf

Rat Bone Marrow-Derived Macrophage Culture

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Primary BMDMs were obtained from rats’ rear leg’s bone marrow and differentiated to macrophages for 6 days in DMEM medium supplemented with 10% heat-inactivated FBS, 1% non-essential amino acids, penicillin, streptomycin, 2 mM glutamine, and 25 ng/ml M-CSF at 37 °C with 5% CO₂.
Human recombinant TNF, Nec-1, zVAD, and BV6 were all synthesized at Genentech. LPS was purchased from InvivoGen, San Diego, CA, USA and TAK1 inhibitor 5z-7-oxozeanol from Bio-Techne Corporation, Minneapolis, MN, USA.

Stark K., Goncharov T., Varfolomeev E., Xie L., Ngu H., Peng I., Anderson K.R., Verschueren E., Choi M., Kirkpatrick D.S., Easton A., Webster J.D., McKenzie B.S., Vucic D, & Bingol B. (2021). Genetic inactivation of RIP1 kinase activity in rats protects against ischemic brain injury. Cell Death & Disease, 12(4), 379.

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Generating Myeloid Progenitor Cells from Bone Marrow

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BM-derived myeloid progenitor cells were generated from hematopoietic progenitor cells isolated from BM using a Miltenyi MACS lineage depletion kit. Cells were stimulated for 3 d in IL-3 (25 ng/ml), IL-6 (25 ng/ml), and SCF (50 ng/ml), washed, and stimulated for indicated times with rIFN-α4 (2,000 U/ml, PBL Assay Science). BMDMs were differentiated by incubating total BM cells in 15-cm tissue culture plates with recombinant M-CSF (100 ng/ml; Genentech) in DMEM for 7-10 d. M-CSF (100 ng/ml) was replenished every 3 d during culturing. BMDMs were plated overnight in M-CSF, rested for 4 h and stimulated for the indicated times with rIFN-α4 (2,000 U/ml; PBL Assay Science). For cell transfection experiments, 3T3 cells were transfected using Lipofectamine LTX with plus reagent (Life Technologies) according to manufacturer’s instruction.

Holmes D.A., Suto E., Lee W.P., Ou Q., Gong Q., Smith H.R., Caplazi P, & Chan A.C. (2015). Autoimmunity-associated protein tyrosine phosphatase PEP negatively regulates IFN-α receptor signaling. The Journal of Experimental Medicine, 212(7), 1081-1093.

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